Skip to main content
Figure 1 | BMC Evolutionary Biology

Figure 1

From: The invariant phenylalanine of precursor proteins discloses the importance of Omp85 for protein translocation into cyanelles

Figure 1

Binding of Cyanophora pFNR and mutants to immobilized lipids. (A) The wild type or mutant pFNR (B, C; bar colour indicated corresponds to label colour on the right site) were incubated with blots coated with 300 pmol of PC or 15 mol% PC and 85 mol% of the indicated lipid (see bottom of C). The binding was quantified, normalized to added protein and to the binding efficiency of wild type pFNR to 300 pmol PC. D) The same experiment as in A was performed using 100 mol% (left bar), 85 mol% (middle bar) or 15 mol% of PC (right bar) supplemented with the indicated lipid. In A-D) the average of at least three independent experiments and the standard deviation is shown. (DGDG, digalactosyldiacylglycerol; LPA, lipid A; MGDG, monogalactosyldiacylglycerol; SQDG, sulfoquinovosyldiacylglycerol; PC, phosphatidylcholine; PG, phosphatidylglycerol; PI, phosphatidylinositol).

Back to article page