Skip to main content

Table 5 Primer sequences used to generate probes

From: Evolution and expansion of the Mycobacterium tuberculosis PE and PPE multigene families and their association with the duplication of the ESAT-6 (esx) gene cluster regions

Probe name

Primer name

Primer sequence (5' to 3')

Application

Rv1917c (and Rv1918c)

ppe-17

ttc aac tcc gtg acg tcg

Amplification of 471 bp 5' terminal region from Rv1917c and Rv1918c

 

ppe-18

cag cac acc ctt gga act g

 

Rv1753c

1753ISH_F

cgg tgg ctt tag tct acc tgc

Amplification of 279 bp 5' terminal region from Rv1753c

 

1753ISH_R

ccg gtc aat gtg tat ggg tg

 

mycP5 (Rv1796)

prot 5 f

gtg ctc gta atg tca tcg

Amplification of 658 bp of Rv1796

 

prot 5 r

cat atc ggc acc ata tcg

Â