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Figure 4 | BMC Evolutionary Biology

Figure 4

From: SmTRC1, a novel Schistosoma mansoni DNA transposon, discloses new families of animal and fungi transposons belonging to the CACTA superfamily

Figure 4

Alternatively spliced forms of SmTRC1 transcripts. A: agarose gel electrophoresis of products from an RT-PCR reaction with S. mansoni mRNA using primers designed from the sequence of the extremities of previously deposited ESTs mapping to the full-length SmTRC1 sequence. The "no RT" lane indicates a control in which no reverse transcriptase was added to the reaction medium. B: full-length SmTRC1 sequence (top scheme) and relative mapping positions of five existing ESTs from GenBank (Accession numbers shown next to each) and of three newly sequenced transcripts obtained by cloning the major band derived from the RT-PCR shown in panel A (Clones B1, B2 and B4 as indicated). Black boxes in the top scheme indicate the Terminal Inverted Repeats (TIR); light gray boxes indicate a predicted SmTRC1-ORF and the dark gray box indicates a Transposase_21 domain within this ORF; the hatched box indicates a region with tandem repeats. Thin black bars below the top scheme indicate mapped exons derived from each transcript; a white box indicates a region of a particular transcript not mapped to this specific copy of the SmTRC1 genomic sequence. Thin continuous lines represent junctions between interconnected exons in the transcripts, defining an intron with the canonical GT-AG splicing sites. Dashed lines represent junctions between interconnected exons in the transcripts, defining an intron without the canonical GT-AG splicing sites. Two "A"s indicate the presence of a poly-A tail. C: schematic representation of 3 clones of SmTRC1 transcripts. The scale in this part of the figure is expanded in comparison to that used in part B above. Light gray boxes indicate predicted ORF. Names inside the boxes indicate different hypothetical protein products coded by those transcripts. The asterisk indicates a stop codon present in the transcript but not in the equivalent genomic sequence of the full-length SmTRC1 element.

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