Figure 6

Additional identified frameshift events in the APETALA3 lineage. A, Amino acid (left) and corresponding nucleotide (right) alignments of the C-terminal regions of select Asterid euAP3 cDNAs. Loci from Antirrhinum (DefA), Syringa (SvAP3) and Petunia (pMADS1) show the typical euAP3 motif but a single nucleotide insertion in the Solanaceous taxa Nicotiana tobaccum (NTDEF), Solanum lycopersicon (LeAP3) and Solanum tuberosum (StDef) has produced a one amino acid truncation. B, Amino acid (left) and corresponding nucleotide (right) alignments of the C-terminal regions of the Paeonia suffructosa TM6 lineage members PesTM6-1 and PesTM6-2. A seven nucleotide deletion in PesTM6-2 has given rise to a novel C-terminal motif that replaces the paleoAP3 motif (which is moderately conserved in PesTM6-1). There is an additional indel between the two loci in the region of the PesTM6-2 stop codon. C, Amino acid (left) and corresponding nucleotide (right) alignments of the C-terminal regions of the Drimys winterii paleoAP3 lineage members DrwAP3-1, -2, -3 and -4. An eight nucleotide deletion in DrwAP3-1 results in remodeling of the last four amino acids in the paleoAP3 motif. For A-C: Asterisks indicate translational stops. The stop codons used in the separate reading frames are boxed. Numbers at right indicate the position in the amino acid or nucleotide sequence of each locus. See also Additional Files 2 and 3.