Mitochondrial and nuclear phylogenetic analysis with Sanger and next-generation sequencing shows that, in Área de Conservación Guanacaste, northwestern Costa Rica, the skipper butterfly named Urbanus belli(family Hesperiidae) comprises three morphologically cryptic species

Table 7 Wolbachia BLAST results from the wsp and MLST database for the five markers: coxA, hpcA, gatB, fstZ , and fbpA

Marker	Allele	Percentage identity match in WSP & MLST databases
wsp	115	100%
gatB	71	98%
coxA	67	100%
ftsZ	65	99%
hcpA	74	99%
fbpA	6	98%

The table includes the allele type that was found in the BLAST search and the percentage identity match in the databases resulting in the Wolbachia strain 108 of Supergroup B.

ISSN: 2730-7182