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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Evolution of a horizontally acquired legume gene, albumin 1, in the parasitic plant Phelipanche aegyptiaca and related species

Figure 2

Maximum likelihood (ML) and Bayesian inference (BI) phylogeny of albumin 1 homologs in broomrape species and legumes. Horizontal acquisition of albumin 1 by an ancestral Phelipanche/Orobanche species was estimated to have occurred ca. 16 million years ago (Mya, with standard errors SE), with Orobanche-Phelipanche speciation ca. 11 Mya, and a gene duplication ca. 5 Mya in the Phelipanche lineage produced xenparalogous genes designated Albumin1-1 (12653) and Albumin1-2 (75797) (see Supplemental Methods). Papilionoid legumes in black, others as indicated. Age estimate of legume node marked by red circle (39 ± 2.4 Mya) taken from Lavin et al. [51]. Unrooted trees have been rooted with Glycine max, in agreement with a prior KNOTTIN phylogeny [30] and phylogenetic relationships of related legume sequences [50]. Tree shown is ML (BI method produced the same tree topology); bootstrap values (if >50%) and posterior probabilities (if >0.60) are shown on internal nodes. The legume clade containing albumin 1 genes is comprised of the Millettioids clade, which contains genera such as Glycine and Phaseolus, as the sister group to the large, temperate Hologalegina clade, which includes Medicago, Pisum, Astragalus and Onobrychis, as well as several other agriculturally important genera such as Cicer, Lens, Vicia, and Trifolium[50]. Legume KNOTTIN sequences were from the KNOTTIN database [31]. For each legume KNOTTIN, tripartite names are given as: species full name-ID from KNOTTIN database-sequence ID from UniProt database. Additional albumin 1 homologs from M. truncatula were retrieved from Medicago truncatula HapMap Project [52] with original sequence IDs. Branches are scaled by number of substitutions. The two albumin 1 genes in Phelipanche aegyptiaca have nt sequence identity 92%.

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