Endosymbiont diversity among sibling weevil species competing for the same resource

Table 2 Primers used for PCR diagnostic

Target Species	Target gene	Primer name	Primer sequence (5’-3’)	Product size (kb)	Temp.^*(°C)	Refs.^†
Host	CytB rRNA	CBJ-10933	TATGTACTACCATGAGGACAAATATC	0.5	45	[42]
(Curculio sp.)		CBN-11367	ATTACACCTCCTAATTTATTAGGAAT			[42]
Eubacterial Universal Primers	16S rRNA	008For	AGA GTT TGA TCA TGG CTC AG	1.5	53	[43]
		1487Rev	TAC CTT GTT ACG ACT TCA CC			(**)
Candidatus Curculioniphilus buchneri	16S rRNA	16S-F	AGAGATCTGGAGGAATATCA	0.4	52	(*)
		16S-R	CACTAAAGCATCTCTGCTAAAT			(*)
	GroEL	GroEL 2 F	ATG GGB GCT CAA ATG GTK AAA	0.9	55	[38]
		GroEL 2R	CTCTTTCATTTCAACTTCNGTBGCA			[38]
Wolbachia	16S rRNA	W-Spec F	CATACCTATTCGAAGGGATAG	0.4	60	[44]
		W-Spec R	AGCTTCGAGTGAAACCAATTC			[44]
Rickettsia	16S rRNA	RbF	GCTCAGAACGAACGCTATC	0.9	58	[12]
		RbR	GAAGGAAAGCATCTCTGC			[12]
Spiroplasma	ITS and 16S rRNA	Spixo-16S F	TTAGGGGCTCAACCCCTAACC	0.8	52	[45]
		Spixo-16S R	TCTGGCATTGCCAACTCTC			[45]
Sodalis	16S rRNA	Sodalis 370 F	CGRTRGCGTTAAYAGCGC	0.2	55	[38]
		16SSod590R	AACAGACCGCCTGCGTACG			[38]

*Annealing temperature. ^†References: (*) this study; (**) nucleotides numbering from Escherichia coli, GenBank accession number J01859.

ISSN: 2730-7182