- Research article
- Open Access
Explosive diversification following a benthic to pelagic shift in freshwater fishes
BMC Evolutionary Biology volume 13, Article number: 272 (2013)
Interspecific divergence along a benthic to pelagic habitat axis is ubiquitous in freshwater fishes inhabiting lentic environments. In this study, we examined the influence of this habitat axis on the macroevolution of a diverse, lotic radiation using mtDNA and nDNA phylogenies for eastern North America’s most species-rich freshwater fish clade, the open posterior myodome (OPM) cyprinids. We used ancestral state reconstruction to identify the earliest benthic to pelagic transition in this group and generated fossil-calibrated estimates of when this shift occurred. This transition could have represented evolution into a novel adaptive zone, and therefore, we tested for a period of accelerated lineage accumulation after this historical habitat shift.
Ancestral state reconstructions inferred a similar and concordant region of our mtDNA and nDNA based gene trees as representing the shift from benthic to pelagic habitats in the OPM clade. Two independent tests conducted on each gene tree suggested an increased diversification rate after this inferred habitat transition. Furthermore, lineage through time analyses indicated rapid early cladogenesis in the clade arising after the benthic to pelagic shift.
A burst of diversification followed the earliest benthic to pelagic transition during the radiation of OPM cyprinids in eastern North America. As such, the benthic/pelagic habitat axis has likely influenced the generation of biodiversity across disparate freshwater ecosystems.
Freshwater fish are frequently thought to diversify along a benthic (bottom) to pelagic (mid-water) habitat axis [1–3]. However, the generality of this pattern has largely been inferred from fishes that inhabit lentic, or lake-like, environments, such as sticklebacks, perch, arctic charr, and cichlids [1–7]. Furthermore, most of these studies have examined microevolutionary processes of interspecific divergence. It remains unclear if benthic/pelagic divergence has commonly influenced macroevolutionary patterns within large clades of fishes inhabiting ecologically complex lotic, or riverine, systems.
Cyprinid fishes have radiated extensively within flowing water environments across eastern North America to exploit both benthic and pelagic habitats. Therefore, this group should provide an ideal study system to test whether the benthic to pelagic habitat axis drives macroevolution in a species-rich group of fishes. Previous phylogenetic analyses have generated a general framework for understanding relationships among these fishes and have shown that most (>95%) of the cyprinid species inhabiting eastern North America form a strongly supported clade [8–12]. This clade is united by the osteological character of a small opening at the base of the skull known as the open posterior myodome (OPM). Previous studies have also generally agreed that a small clade of seven species, with two distributed in eastern North America (Clinostomus elongatus and C. funduloides) and five endemic to western North America (Iotichthys phlegethontis, Mylocheilus caurinus, Pogonichthys macrolepidotus, Richardsonius balteatus, and R. egregius) form the sister group to a much larger group of species that is primarily confined to eastern North America [9–11]. Within this eastern radiation a strongly supported clade of around 200 predominantly pelagic species that display terminal mouths and feed generally from the mid-water is consistently recovered as arising following the initial divergence of several depauperate and strictly benthic lineages that display inferior mouths and often posses maxillary barbels [9–11]. However, phylogenetic ambiguity remains in the branching order of these early benthic lineages, and the phylogenetic affinities of many species that are thought to lie within the predominately pelagic clade have not been resolved [8–12]. Therefore, a much more exhaustively sampled phylogeny combined with data on benthic/pelagic habitat use should facilitate a more robust phylogenetic examination of whether this ecological axis has influenced diversification within OPM cyprinids.
Habitat divergence clearly promotes coexistence in many lotic systems. For instance, OPM cyprinids often form complex communities consisting of up to 15 species that partition the water column into vertically stratified foraging zones [13–16]. Furthermore, small, insectivorous or omnivorous fishes from other groups are relatively rare in the pelagic zone of rivers and streams in eastern North America . Therefore, the first transition from a benthic to pelagic habitat in OPM cyprinids likely represented the invasion of a sparsely occupied adaptive zone that could have resulted in a period of accelerated diversification [17–20]. Given the apparent influence of the benthic/pelagic axis on community structure, mapping this habitat divergence onto the OPM phylogeny could highlight its role in generating species diversity.
Hypotheses addressing the ecological mechanisms that have influenced historical patterns of diversification can now be examined using robustly sampled molecular phylogenies and applying methods that examine phylogenetic tree shape . Acceleration in diversification rate is often thought to result from rapid divergence following invasion of open adaptive zones in groups ranging from vertebrates to prokaryotes [22–26]. Yet within freshwater fishes, several tests for ecologically associated bursts of diversification have failed to reject a constant rate of cladogenesis [27–29]. However, OPM cyprinids could have experienced an exceptional period of lineage diversification following their initial transition from benthic to pelagic habitats.
In this study, we generated the most thoroughly sampled, species-level phylogenetic hypotheses for OPM cyprinids using DNA sequence data from both a mitochondrial and nuclear marker. We then used ancestral state reconstruction and fossil-calibrated divergence time estimates to infer the history of benthic/pelagic habitat use across our phylogenetic reconstructions. Using several independent methods, we addressed the question: Was the first major evolutionary shift from benthic to pelagic habitats in eastern North America followed by a period of accelerated lineage diversification in OPM cyprinids?
Phylogenetic analysis of the cytochrome b (Cytb) and recombination activating gene 1 exon 3 (Rag1) loci provided substantial resolution of relationships among members of the OPM radiation (Additional file 1). Both maximum clade credibility (MCC) gene trees and the MCC concatenated analysis included moderately to well-supported clades (>90% posterior probability (pp)) containing the benthic genera Campostoma, Exoglossum, Nocomis, and Rhinichthys as the earliest diverging OPM lineages in eastern North America (Additional file 1 and Figures 1 and 2). Both gene trees recovered the remaining eastern benthic genera Dionda, Erimystax, Macrhybopsis, Phenacobius, and Platygobio as diverging before the diversification of the strongly supported (100% pp) focal clade (see Methods) that is dominated by pelagic species and accounts for approximately 80% of extant OPM diversity. This general topology was strongly supported in the concatenated analysis as well (Figures 1 and 2). Some phylogenetic relationships within the predominately pelagic focal clade were variable between our two gene trees and the concatenated analysis, with many clades receiving varying levels of posterior support. For instance, we consistently recovered poorly resolved nodes and short internode branch lengths at the base of the focal clade.
Ancestral state reconstruction and divergence time estimates
Ancestral state reconstruction points to a similar node in both of our gene trees as representing the initial shift from benthic to pelagic habitat utilization in the eastern OPM radiation (Figure 3 and Additional file 1). In the more thoroughly sampled Cytb gene tree, this shift is inferred to have occurred along a branch leading to the most recent common ancestor (MRCA) of a strongly supported focal clade. We considered the node representing this MRCA as the transition node and conducted our tests for variation in diversification rate and an excess of early lineages after the habitat shift with respect to this node. In the Rag1 gene tree, we recovered that the shift occurred at a slightly more ancestral node. This node was subtended by a clade containing the same set of genera as the Cytb focal clade plus its sister group Macrhybopsis spp. + Platygobio gracilis. However, this node received poor support (62% pp) in the Rag1 gene tree. Furthermore, the subsequent node in the Rag1 tree that was subtended by the same set of genera as the Cytb focal clade was strongly supported (100% pp) and had a higher likelihood of being pelagic. Preliminary analyses suggested that using this node as opposed to its poorly supported ancestral node had little impact on our calculation of diversification rate and tree shape statistics. Therefore, we considered this to be the transition node for the Rag1 topology as well. We estimated the age of the transition nodes to be 33 mya (95% highest posterior density: 16–47 mya) based on Cytb (Figure 4), 27 mya (95% highest posterior density: 16–61 mya) based on Rag1.
Following the initial benthic to pelagic shift early in the history of the OPM radiation, ancestral state reconstruction recovered several instances of the re-evolution of benthicity within the pelagic focal clade (Figure 3). Examples of lineages that have re-evolved benthic specialization within this pelagic clade include the barbeled genus Hybopsis as well as a sister species pair of barbeled Cyprinella, C. labrosa and C. zanema. Based on the more thoroughly sampled Cytb MCC topology, we recovered approximately six transitions back to a benthic habitat during the history of the clade. After these transitions back to benthicity, transitions back to pelagic habitats were inferred to be very rare (Figure 3).
Diversification rate analyses
Our strategies used to examine topological inbalance and variation in diversification rate across the entire OPM phylogeny supported the hypothesis that there was accelerated diversification following the initial benthic to pelagic transition in the OPM radiation. The relative cladogenesis (RC) test identified 15 nodes associated with significantly diverse subclades in our Cytb MCC gene tree (Figure 3A). These nodes included two that are immediately ancestral to the transition node at the base of the focal clade, the transition node itself, and 12 consecutive descendent nodes. The parametric rates comparison (PRC) analysis marginally supported a model with a higher diversification rate in the predominately pelagic focal clade relative to the remainder of the Cytb gene tree (p = 0.06) (Figure 4A). We also found that three nodes immediately following the transition node represented clades that were significantly more likely to be modeled as having a greater diversification rate relative to the remainder of the tree at α = 0.1.
Likewise, our examinations of deviations from a constant rate of cladogenesis as compared to randomly generated pure-birth topologies also supported the hypothesis that there was a burst of diversification coincident with the initial shift to a pelagic habit. The γ statistic was significant for our focal clade in both the Cytb and Rag1 MCC gene trees (Table 1) indicating an excess of early lineages in this clade. The Cytb LTT plot for the focal clade lay largely outside the 95% confidence intervals for nearly the entire history of 10,000 simulated pure-birth trees (Figure 4B). The Monte Carlo constant rates (MCCR) analyses indicated a strong deviation from a pure-birth process based on Cytb and the MCCR analyses marginally supported this deviation in the Rag1 gene tree (Table 1). Tree deviation scores were also significant on our two gene trees, again indicating an excess of early lineages in our focal clade (Table 1). Finally, variable-rate models provided a significantly better fit than constant-rate models to the observed vectors of branching times within the predominately pelagic focal clade (Table 1). We obtained similar results when we applied these test to the 9005 post burn-in trees (Additional file 2).
The initial evolutionary transition from benthic to pelagic habitats by OPM cyprinid fishes likely had a significant impact on the diversification of this hyper-diverse clade of fishes. Our two phylogenetic hypotheses, coupled with ancestral state reconstructions and divergence time estimates, indicated that benthic forms dominated the early history of the eastern OPM radiation. This group then gave rise to a predominately pelagic clade that began diversifying around 30 mya and contains ~80% of extant OPM species. Our tests for increased diversification rate all highlighted the particular region in the phylogeny where the earliest benthic to pelagic habitat shift is inferred to have occurred. With our thoroughly sampled phylogenies, we were also able to reject a pattern of constant-rate cladogenesis in favor of models that are consistent with a period of accelerated diversification after this habitat shift. Importantly, we did not test whether pelagic clades are generally more diverse than benthic cyprinid clades, but we did find that the evolution of the potential for exploiting the pelagic habitat appears to have led to a shift in diversification rates. As such, the OPM cyprinids’ first benthic to pelagic transition likely represented evolution into an open adaptive zone that resulted in a period of rapid lineage accumulation [17–19].
In the area of the phylogeny immediately following this inferred benthic to pelagic transition, we were not able to confidently resolve relationships among lineages. This region has often been unresolved in other studies of OPM evolution [8–12]. The explosive diversification in this region of the tree likely has contributed to this phylogenetic ambiguity . Future phylogenetic studies based on large, multi-locus datasets that utilize a species tree framework could potentially help to resolve these problematic areas of the OPM phylogeny . However, determining the exact branching order of lineages whose divergence is coincident with major ecological shifts and periods of rapid diversification might be generally difficult.
The major shift from benthic to pelagic habitats in OPM cyprinids should not be considered in isolation from the other freshwater fish diversity in eastern North America. The diversification in the predominately pelagic focal clade that began around 30 mya coincides with the estimated age of the darter (Percidae: Etheostomatinae) radiation . Darters are another endemic North American freshwater group of around 250 benthic fishes that often co-occur with OPM species [13, 30]. Cyprinids and darters together dominate the abundance and species diversity in most eastern North America fish assemblages . With the exception of a few omnivorous species, OPM cyprinids and darters are both primarily insectivorous and compete for similarly sized prey . Therefore, darter diversification might have further reduced eco-evolutionary opportunities within benthic habitats and influenced the shift of the OPM lineage into the relatively competitor-free pelagic zone. A macroevolutionary interaction between these two lineages could have contributed to the observed pelagic burst of OPM diversity.
Interspecific competition, however, might not have been the only mechanism driving the rapid diversification of pelagic OPM cyprinids. For instance, there is also an increase in the presence of male nuptial coloration and sexual dichromatism in the more visually dependent pelagic OPM species relative to their benthic relatives that rely more extensively on chemical cues during foraging . Given this, an increase in visually mediated sexual selection could have also played a role in the diversification of the pelagic OPM cyprinids [32, 33]. Additionally, an interaction between ecological opportunity and sexual selection might have driven the increased rate of lineage accumulation that followed the first benthic to pelagic transition in this group .
Evolution along the benthic/pelagic habitat axis appears to have played a critical role in generating the impressive species numbers of OPM cyprinids inhabiting the lotic systems of eastern North America. Future studies of other freshwater fish groups that combine ecological data with more thoroughly sampled phylogenies and examinations of temporal shifts in diversification could provide additional evidence that divergence along this axis has repeatedly influenced fish macroevolution. Our results indicate that the influence of this habitat axis is clearly not restricted to lentic environments [1–7]. Instead, the benthic/pelagic axis of diversification appears to be a ubiquitous generator of biodiversity across disparate freshwater ecosystems.
Phylogenetic and divergence time analyses
We used a combination of FishBase  and Page and Burr  to generate a list of the currently recognized species of OPM cyprinids. Using DNA sequences downloaded from GenBank combined with new sequence data, we constructed manually aligned matrices for the mitochondrial Cytb and nuclear Rag1 loci. To obtain new sequence data, we first used DNAeasy Tissue Extraction Kits (Qiagen, Valencia, CA) to extract genomic DNA from tissue samples. Cytb was amplified using primers from Schmidt and Gold . Rag1 was amplified using primers from Lopez et al. . DNA sequencing was performed at the University of Washington’s High Throughput Genomics Unit using the PCR primers and an internal primer to sequence Rag1, IF4: 5′-TGAGAAGGCAGTGAGGTTTT-3′. We created contiguous sequence files from directional sequence reads using Sequencher 4.8 (Gene Codes, Ann Arbor, MI, USA) and coded heterozygous sites in the Rag1 alignment as ambiguous. The Cytb alignment (1060–1140 bp) included data for 223 of the 238 (94%) extant OPM taxa. The Rag1 alignment (1440–1518 bp) included data for 187 of the 238 (79%) extant OPM taxa. Our sampling includes taxa from throughout the geographic range of the clade with no obvious sampling bias between benthic and pelagic taxa (Additional file 3). We deposited all new sequence data on GenBank [GenBank: KC763652-KC763776] (Additional file 3). This includes 47 novel Cytb sequences and 78 novel Rag1 sequences.
We estimated the phylogenies and divergence times for each of our two loci separately utilizing BEAST v1.7.1 . We first defined codon positions in our two alignments using MacClade v4.07  and then assigned the best model based on AIC scores calculated by jModelTest v0.1  to each gene’s codon sites in BEAUti v1.7.1 . Substitution rate, rate heterogeneity, and base frequency parameters were treated as unlinked across partitions. We used a birth-death speciation prior for our tree models. The OPM clade containing Mylocheilus caurinus, Pogonichthys macrolepidotus, Clinostomus funduloides, Clinostomus elongatus, Iotichthys phlegethontis, Richardsonius balteatus, and Richardsonius egregious was included as the outgroup to the eastern OPM radiation in our phylogenetic analyses [9–11]. We conducted a single heuristic likelihood tree search on each gene alignment using RAxML v7.0.4  to generate starting trees for our MCMC runs. We also concatenated the two alignments and ran a phylogenetic analysis on this combined matrix using the same models specified in the individual gene analyses.
To estimate divergence times within the OPM radiation, we used an uncorrelated lognormal molecular clock model to temporally calibrate our two gene trees and concatenated phylogeny. Based on previous results [9–11], we constrained the monophyly of Mylocheilus caurinus and Pogonichthys macrolepidotus and then defined a fossil-calibrated prior distribution on the age of their MRCA. The fossil species Mylocheilus whitei was used to infer a minimum age estimate for this split. This fossil is a pharyngeal arch with a short anterior limb and thick internal ridges displaying small canals and pores, as well as molariform dentition, which are characters that are diagnostic for Mylocheilus. The fossil was recovered from a geological layer representing the Clarendonian/Hemphillian boundary at approximately 9 million years ago (mya) . We therefore specified a lognormal prior for the MRCA of Mylocheilus caurinus and Pogonichthys macrolepidotus with a mean and standard deviation of 1 mya and offset by 9 mya. The root node ages of our trees were constrained using a uniform prior with an upper bound of 75 mya based on recent MRCA age estimates of Cyprinidae . Our MCMC chains were run for 2.0 × 107 generations, with trees and parameter estimates logged every 1.0 × 104 generations. We then ran each MCMC search five times using the CIPRES Science Gateway . The first 10% of each run was discarded as the burn-in. Subsequently, we examined ESS values in TRACER v1.5  over the remainder of the run to ensure convergence of parameter estimates. We combined log and tree files using LogCombiner v1.7.1 and Tree Annotator v1.7.1  to calculate the maximum clade credibility (MCC) tree for each locus and the concatenated analysis. All trees are deposited in TreeBASE (http://purl.org/phylo/treebase/phylows/study/TB2:S15034).
Habitat designations and ancestral state reconstruction
To estimate the most likely ancestral node representing the first benthic to pelagic shift of OPM cyprinids in eastern North America, we first designated extant taxa as benthic (0) or pelagic (1) based on a combination of morphological and ecological characteristics (Additional file 3). Taxa coded as benthic display some combination of the following characteristics: 1) mouth is located ventrally 2) possess barbels 3) exhibit a spiraled gut 4) build benthic nests and 5) feed primarily on benthic food items. We coded taxa that do not display any of these traits as pelagic. We then used Pagel’s  single-rate Markov model of binary character evolution and assumed equal transition probabilities to reconstruct benthic/pelagic ancestral states using the package ape v3.0  in R . We considered the most ancestral node inferred to have a greater than 50% probability of being pelagic to represent the initial benthic to pelagic transition in our phylogenies. This method of habitat coding and ancestral state reconstruction provided a conservative approach for inferring the phylogenetic placement of the benthic/pelagic shift on our gene trees. All taxa that could possibly be benthic were coded as such. Furthermore, all taxa diverging before the ‘transition node’ are unambiguously benthic. Therefore, any possible alternative codings would include more pelagic taxa in the focal clade and would result in the same nodes in our gene trees being recovered as the ‘transition node’.
Diversification rate analyses
We employed two strategies to test for a period of accelerated diversification following the first benthic to pelagic transition in the OPM radiation of eastern North America. First, we used the entire Cytb gene tree and conducted the relative cladogenesis (RC) test  to identify significantly diverse subclades using the R package geiger v1.0 . Using a homogeneous model of cladogenesis, this test examines the number of lineages alive just before a node and the number of lineages descending from the node and calculates the probability that the node has as many descendants as it has empirically [49, 50]. We also used the parametric rates comparison (PRC) test of Shah et al.  implemented in the R package iteRates v3.0. This method iterates across all subtrees within the phylogeny that contain at least 6 edges, fits distributions to the vector of branch lengths within each subtree, and compares the likelihood that the vector of branch lengths from each subtree is best modeled as being drawn from the same, or different, distribution as the remainder of the tree. We fit an exponential distribution to our vector of branching times in the PRC analysis. Both of these methods, RC and PRC, assume complete taxon sampling [49, 51]. Therefore, we only conducted these tests on the more robustly sampled Cytb MCC gene tree. Both taxonomic inflation and using a single individual per species can bias these types of analyses because of obscured patterns of cladogenesis at the tips of the gene tree [23, 52]. Therefore, we truncated the most recent five million years from the Cytb tree before conducting these tests using the treeTrim function in iteRates v3.0.
We next focused on the predominately pelagic clade subtending the ‘transition node’ (Figure 3), which we refer to as the ‘focal clade’. We used several independent analyses to test for an early period of rapid cladogenesis in this clade using a combination of the R packages geiger v1.0  and laser v2.2 . We first used Pybus and Harvey’s  constant rates test on our two focal clade phylogenies. This test is frequently called the γ test based on its test statistic, γ, which is distributed as a standard normal variable under a pure-birth process . Values of γ < -1.645 are considered significant deviations from pure-birth with diversification events clustered towards the base of a tree.
However, our phylogenies only included 91% (Cytb) and 74% (Rag1) of the recognized species diversity in the focal clade and incomplete taxon sampling will bias our calculations of γ because incomplete lineage sampling prunes tips from the tree, thereby inflating the branch lengths in the recent past . To correct for this bias, we employed Pybus and Harvey’s  Monte Carlo constant rates test (MCCR test), where the critical value for rejecting a constant rate (at α = 0.05) is calculated by examining the distribution of γ for simulated trees that include incomplete taxon sampling. Our null distribution of γ was calculated from 1 million simulated pure-birth trees of 192 taxa, or the number of described species that belong to the genera comprising the focal clade. Our simulated trees were corrected for the number of taxa missing in our reconstructed Cytb and Rag1 phylogenies by randomly pruning 18 and 50 taxa from each simulated tree, respectively. All phylogenetic simulations used a modification of the birthdeath.tree function in geiger v1.0 to ensure that the trees had the desired statistical properties (see  for details). Additionally, we calculated the ‘tree deviation’ statistic , which can have greater power to detect accelerated diversification early in the history of a tree by examining if lineages have accumulated at a greater rate than predicted by a null distribution. The null distribution for the ‘tree deviation’ was calculated from 1 million simulated pure-birth trees with incomplete taxon sampling. We also generated a lineage through time plot (LTT) for our focal clade based on the more thoroughly sampled Cytb MCC gene tree to compare to a distribution of 10,000 simulated pure-birth LTT plots.
We then used a likelihood-based approach to test for a deviation from a constant-rate pattern of diversification in the focal clade. We fit two constant-rate models (pure-birth, birth-death) and three variable-rate models (density dependent logistic, density dependent exponential, and Yule 2-rate) to the vector of branching times from the two focal clade phylogenies. To determine the best-fit model for our data and to account for incomplete taxon sampling, we used the method proposed by Rabosky . This method compares the observed ∆AIC between the best-fit constant-rate model and the best-fit variable-rate model of a focal tree to the 0.95 quantile of a null distribution of ∆AIC values calculated from 1 million simulated pure-birth phylogenies with incomplete lineage sampling. The constant rates test , tree deviation , and model fitting approach of Rabosky  were applied to the MCC gene trees for Cytb and Rag1, and also across all post burn-in trees from the BEAST analysis.
Availability of supporting data
All trees are deposited in TreeBASE (http://purl.org/phylo/treebase/phylows/study/TB2:S15034). Newly generated sequence data is available on GenBank [KC763652-KC763776].
Robinson BW, Wilson DS: Character release and displacement in fishes: a neglected literature. Am Nat. 1994, 144: 596-627.
Willacker JJ, von Hippel FA, Wilton PR, Walton KM: Classification of threespine stickleback along the benthic-limnetic axis. Biol J Linn Soc. 2010, 101: 595-608.
Hulsey CD, Roberts RJ, Loh YHE, Rupp MF, Streelman JT: Lake Malawi cichlid evolution along a benthic/limnetic axis. Ecol Evol. 2013, 3: 2262-2272.
Skúlason S, Noakes DLG, Snorrason SS: Ontogeny of trophic morphology in four sympatric morphs of arctic charr Salvelinus alpinus in thingvallavatn, Iceland. Biol J Linn Soc. 1989, 38: 281-301.
Schluter D: Adaptive radiation in sticklebacks: size, shape, and habitat use efficiency. Ecology. 1993, 74: 699-709.
Svanbäck R, Eklöv P: Morphology dependent foraging efficiency in perch: a trade-off for ecological specialization?. Oikos. 2003, 102: 273-284.
Meyer A: Ecological and evolutionary consequences of the trophic polymorphism in Cichlasoma citrinellum (Pisces: Cichlidae). Biol J Linn Soc. 1990, 39: 279-299.
Mayden RL: Phylogenetic studies of north american minnows, with emphasis on the genus Cyprinella (teleostei: cypriniformes). Misc Pub Univ Kans Mus Nat Hist. 1989, 80: 1-189.
Simons AM, Berendzen PB, Mayden RL: Molecular systematics of North American phoxinin genera (Actinopterygii: cyprinidae) inferred from mitochondrial 12S and 16S ribosomal RNA sequences. Zool J Linn Soc. 2003, 139: 63-80.
Bufalino AP, Mayden RL: Phylogenetic evaluation of North American leuciscidae (Actinopterygii: cypriniformes: cyprinoidea) as inferred from analyses of mitochondrial and nuclear DNA sequences. Syst Biodivers. 2010, 8: 493-505.
Houston DD, Shiozawa DK, Riddle BR: Phylogenetic relationships of the western North American cyprinid genus Richardsonius, with an overview of phylogeographic structure. Mol Phylogenet Evol. 2010, 55: 259-273.
Hollingsworth PR, Hulsey CD: Reconciling gene trees of eastern North American minnows. Mol Phylogenet Evol. 2011, 61: 149-156.
Page LM, Burr BM: Peterson field guide to freshwater fishes. 2011, Boston: Houghton Mifflin, 2
Baker JA, Ross ST: Spatial and temporal resource utilization by southeastern cyprinids. Copeia. 1981, 1981: 178-189.
Gorman OT: The dynamics of habitat use in a guild of Ozark minnows. Ecol Monogr. 1988, 58: 1-18.
Gorman OT: An experimental study of habitat use in an assemblage of Ozark minnows. Ecology. 1988, 69: 1239-1250.
Simpson GG: The major features of evolution. 1953, New York: Columbia University Press
Schluter D: The ecology of adaptive radiations. 2000, Oxford: Oxford University Press
Losos JB: Adaptive radiation, ecological opportunity, and evolutionary determinism. Am Nat. 2010, 175: 623-639.
Hulsey CD, Hollingsworth PR: Do constructional constraints influence cyprinid (Cyprinidae: leuciscinae) craniofacial coevolution?. Biol J Linn Soc. 2011, 103: 136-146.
Glor RE: Phylogenetic insights on adaptive radiation. Annu Rev Ecol Evol Syst. 2010, 41: 251-270.
Harmon LJ, Melville J, Larson A, Losos JB: The role of geography and ecological opportunity in the diversification of day geckos (Phelsuma). Syst Biol. 2008, 57: 562-573.
Rabosky DL, Lovette IJ: Density-dependent diversification in North American wood warblers. Proc R Soc B. 2008, 275: 2363-2371.
Dumont ER, Dávalos LM, Goldberg A, Santana SE, Rex K, Voigt CC: Morphological innovation, diversification, and invasion of a new adaptive zone. Proc R Soc B. 2012, 279: 1797-1805.
Fordyce JA: Host shifts and evolutionary radiations of butterflies. Proc R Soc B. 2010, 277: 3735-3743.
Morlon H, Kemps BD, Plotkin JB, Brisson D: Explosive radiation of a bacterial species group. Evolution. 2012, 66: 2577-2586.
Day JJ, Cotton JA, Barraclough TG: Tempo and mode of diversification of lake Tanganyika cichlid fishes. PLoS One. 2008, 3: e1730-
Hulsey CD, Hollingsworth PR, Fordyce JA: Temporal diversification of Central American cichlids. BMC Evol Biol. 2010, 10: 279-
Day JJ, Peart CR, Brown KJ, Friel JP, Bills R, Moritz T: Continental diversification of an African catfish radiation (Mochokidae: Synodontis). Syst Biol. 2013, 62: 351-365.
Near TJ, Bossu CM, Bradburd GS, Carlson RL, Harrington RC, Hollingsworth PR, Keck BP, Etnier DA: Phylogeny and temporal diversification of darters (Percidae: etheostomatinae). Syst Biol. 2011, 60: 565-595.
Knight RR, Gregory MB, Wales AK: Relating streamflow characteristics to specialized insectivores in the Tennessee river valley: a regional approach. Ecohydrol. 2008, 1: 394-407.
Barraclough TG, Harvey PH, Nee S: Sexual selection and taxonomic diversity in passerine birds. Proc R Soc B. 1995, 259: 211-215.
Kazancıoğlu E, Near TJ, Hanel R, Wainwright PC: Influence of sexual selection and feeding functional morphology on diversification rate of parrotfishes (Scaridae). Proc R Soc B. 2009, 276: 3439-3446.
Wagner CE, Harmon LJ, Seehausen O: Ecological opportunity and sexual selection together predict adaptive radiation. Nature. 2012, 487: 366-369.
Froese R, Pauly D: FishBase 2000: concepts, design and data sources. 2000, Los Baños: ICLARM
Schmidt TR, Gold JR: Complete sequence of the mitochondrial cytochrome-b gene in the cherryfin shiner, Lythrurus roseipinnis (Teleostei, cyprinidae). Copeia. 1993, 1993: 880-883.
Lopez JA, Chen W-J, Orti G: Esociform phylogeny. Copeia. 2004, 2004: 449-464.
Drummond AJ, Suchard MA, Xie D, Rambaut A: Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol. 2012, 29: 1969-1973.
Maddison DR, Maddison WP: MacClade version 4: analyses of phylogeny and character evolution. 2000, Sunderland: Sinauer Associates
Posada D: jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008, 25: 1253-1256.
Stamatakis A, Ludwig T, Meier H: RAxML-III: a fast program for maximum likelihood-based inference of large phylogenetic trees. Bioinformatics. 2005, 21: 456-463.
Smith GR, Cossel J: Fishes from the late miocene poison creek and chalk hills formations, owyhee county, Idaho. And whereas…papers on the vertebrate paleontology of Idaho honoring John A. White, Volume 2. Edited by: Akersten WA, Thompson ME, Meldrum DJ, Raup RA, McDonald HG. 2002, Pocatello: Idaho Museum of Natural History, 23-35.
Near TJ, Eytan RI, Dornburg A, Kuhn KL, Moore JA, Davis MP, Wainwright PC, Friedman M, Smith WL: Resolution of ray-finned fish phylogeny and timing of diversification. Proc Natl Acad Sci U S A. 2012, 109: 13698-13703.
Miller MA, Pfeiffer W, Schwartz T: Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Proceedings of the Gateway Computing Environments Workshop. 2010, New Orleans, 1-8.
Rambaut A, Drummond AJ: Tracer v1.4. http://beast.bio.ed.ac.uk/Tracer,
Pagel M: Detecting correlated evolution on phylogenies: a general method for the comparative analysis of discrete characters. Proc R Soc B. 1994, 255: 37-45.
Paradis E, Claude J, Strimmer K: APE: analyses of phylogenetics and evolution in R language. Bioinformatics. 2004, 20: 289-290.
R Development Core Team: R: a language and environment for statistical computing. Vienna, Austria: R Foundation for Statistical Computing, http://www.r-project.org/,
Purvis A, Nee S, Harvey PH: Macroevolutionary influence from primate phylogeny. Proc R Soc B. 1995, 260: 329-333.
Harmon LJ, Weir JT, Brock CD, Glor RE, Challenger W: GEIGER: investigation evolutionary radiations. Bioinformatics. 2008, 24: 129-131.
Shah P, Fitzpatrick BM, Fordyce JA: A parametric method for assessing diversification -rate variation in phylogenetic trees. Evolution. 2013, 67: 368-377.
Isaac NJB, Mallet J, Mace GM: Taxonomic inflation: its influence on macroecology and conservation. Trends Ecol Evol. 2004, 19: 464-469.
Rabosky DL: LASER: a maximum likelihood toolkit for detecting temporal shifts in diversification rates from molecular phylogenies. Evol Bioinform Online. 2006, 2: 257-260.
Pybus OG, Harvey PH: Testing macro-evolutionary models using incomplete molecular phylogenies. Proc R Soc B. 2000, 267: 2267-2272.
Fordyce JA: Interpreting the gamma statistic in phylogenetic diversification rate studies: a rate decrease does not necessarily indicate an early burst. PLoS One. 2010, 5: e11781-
Rabosky DL: Likelihood methods for inferring temporal shifts in diversification rates. Evolution. 2006, 60: 1152-1164.
We thank Benjamin Keck, Morgan Douglas, and Maxwell Rupp for comments on an earlier version of this manuscript. We also thank Gabriela Hogue, Morgan Raley, and Wayne Starnes at the North Carolina Museum of Natural Science, as well as Tom Near and Greg Watkins-Colwell of the Yale Peabody Museum, for providing tissue samples of specimens used in this study. The Department of Ecology and Evolutionary Biology at the University of Tennessee, Knoxville (UTK), as well as the Cokkinias and Hollaender Graduate Fellowships provided funding for this study.
The authors declare that they have no competing interests.
PH conceived the study, performed the molecular lab work, conducted the statistical analyses, and drafted the manuscript. AS contributed sequence data. JF participated in the statistical analyses. CH helped to draft the manuscript. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: (A) Cyt b and (B) Rag1 MCC gene trees for the primarily eastern North American OPM cyprinid radiation. Numbers at nodes represent posterior probability values (pp). Asterisks denote 100% pp. The ‘transition nodes’ indicated by black arrows correspond to those in Figure 3. (PDF 5 MB)
Additional file 3: A list of currently recognized North American OPM cyprinid species, including GenBank accession numbers for previously published and newly sequenced data utilized in this study. Benthic/pelagic designations are based on a number of morphological and ecological characters, and references for these characters are given below the table. (PDF 136 KB)
About this article
Cite this article
Hollingsworth, P.R., Simons, A.M., Fordyce, J.A. et al. Explosive diversification following a benthic to pelagic shift in freshwater fishes. BMC Evol Biol 13, 272 (2013) doi:10.1186/1471-2148-13-272
- Divergence times
- Open adaptive zone