Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae), the Cayenne tick: phylogeography and evidence for allopatric speciation

Table 6 DNA polymorphism statistics and neutrality tests

	Clade	NW	CO	NE	EA	AR	PE	Total
12SrDNA	Sample size/H	51/9	7/2	16/2	15/6	10/3	24/4	123/24
	Hd ± SD	0.58 ± 0.07	0.48 ± 0.17	0.23 ± 0.13	0.85 ± 0.06	0.64 ± 0.10	0.49 ± 0.11	0.83 ± 0.03
	π(%) ± SD	0.24 ± 0.04	0.14 ± 0.05	0.14 ± 0.07	1.2 ± 0.15	0.22 ± 0.05	0.16 ± 0.06	7.6 ± 0.3
DL	Sample size/H	46/2	6/1	14/5	15/10	10/4	19/3	110/25
	Hd ± SD	0.08 ± 0.05	0	0.50 ± 0.16	0.91 ± 0.06	0.73 ± 0.10	0.20 ± 0.12	0.81 ± 0.03
	π(%) ± SD	0.02 ± 0.00	0	0.59 ± 0.17	0.95 ± 0.13	0.47 ± 0.46	0.05 ± 0.03	7.0.4 ± 0.33
COII	Sample size/H	24//4	3/1	12/6	11/7	5/5	5/3	60/25
	Hd ± SD	0.57 ± 0.09	0	0.75 ± 0.12	0.91 ± 0.07	1 ± 0.13	0.70 ± 0.22	0.92 ± 0.02
	π(%) ± SD	0.39 ± 0.09	0	0.33 ± 0.10	0.60 ± 0.62	0.36 ± 0.07	1.44 ± 0.80	9.95 ± 0.63
ITS2	Sample size/H	20/5	7/1	15/1	18/5	5/3	9/4	74/19
	Hd ± SD	0.44 ± 0.13	0	0	0.77 ± 0.05	0.70 ± 0.22	0.75 ± 0.11	0.90 ± 0.02
	π(%) ± SD	0.09 ± 0.03	0	0	0.24 ± 0.05	0.10 ± 0.04	0.12 ± 0.03	3.60 ± 0.27
	Fu’s F	-1.912	-	-	0.39	-0.829	-1.039	14.341
	R2	0.1074	-	-	0.1439	0.2449	0.1667	0.1461
mtDNA total	Sample size/H	17/6	2/2	9/4	8/8	5/5	3/3	44/27***
	Hd ± SD	0.82 ± 0.06	1.00 ± 0.25	0.69 ± 0.15	1.00 ± 0.06	1.00 ± 0.13	1.00 ± 0.27	0.94 ± 0.02***
	π(%) ± SD	0.22 ± 0.05	0.08 ± 0.04	0.32 ± 0.13	0.84 ± 0.15	0.36 ± 0.09	0.21 ± 0.06	8.4 ± 0.61
	Fu’s F	0.347	-	2.112	-2.011	-1.481	-	10.292
	R2	0.1324	-	0.1797	0.1583	0.2111	-	0.1626

Sample size and diversity measures for each gene and for each clade. Hd = haplotype diversity, Gd = gene diversity, H = number of haplotypes or genotypes, π = nucleotide diversity in %, SD = standard deviation. Results of Rozas-Onsins’s (R2) and Fu’s F neutrality tests were recorded for the combined mitochondrial and the nuclear datasets. NW = Ecuador, Costa Rica, Mexico, Texas; CO = Colombia; NE = French Guiana + Venzuela + Brazil (Rondonia); EA = Brazil (Atlantic Coast, Mato Grosso) + Argentina (Yungas); AR = Agentina + Paraguay (Chaco); PE = Perú. *** = p < 0.001. 12SrDNA = small mitochondrial ribosmal subunit, COII = cytochrome oxidase c subunit II, DL = control region or d-loop, ITS2 = intergenic spacer 2, mtDNA = concatenated mitochondrial genes.

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