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Table 6 DNA polymorphism statistics and neutrality tests

From: Amblyomma cajennense (Fabricius, 1787) (Acari: Ixodidae), the Cayenne tick: phylogeography and evidence for allopatric speciation

  Clade NW CO NE EA AR PE Total
12SrDNA Sample size/H 51/9 7/2 16/2 15/6 10/3 24/4 123/24
  Hd ± SD 0.58 ± 0.07 0.48 ± 0.17 0.23 ± 0.13 0.85 ± 0.06 0.64 ± 0.10 0.49 ± 0.11 0.83 ± 0.03
  π(%) ± SD 0.24 ± 0.04 0.14 ± 0.05 0.14 ± 0.07 1.2 ± 0.15 0.22 ± 0.05 0.16 ± 0.06 7.6 ± 0.3
DL Sample size/H 46/2 6/1 14/5 15/10 10/4 19/3 110/25
  Hd ± SD 0.08 ± 0.05 0 0.50 ± 0.16 0.91 ± 0.06 0.73 ± 0.10 0.20 ± 0.12 0.81 ± 0.03
  π(%) ± SD 0.02 ± 0.00 0 0.59 ± 0.17 0.95 ± 0.13 0.47 ± 0.46 0.05 ± 0.03 7.0.4 ± 0.33
COII Sample size/H 24//4 3/1 12/6 11/7 5/5 5/3 60/25
  Hd ± SD 0.57 ± 0.09 0 0.75 ± 0.12 0.91 ± 0.07 1 ± 0.13 0.70 ± 0.22 0.92 ± 0.02
  π(%) ± SD 0.39 ± 0.09 0 0.33 ± 0.10 0.60 ± 0.62 0.36 ± 0.07 1.44 ± 0.80 9.95 ± 0.63
ITS2 Sample size/H 20/5 7/1 15/1 18/5 5/3 9/4 74/19
  Hd ± SD 0.44 ± 0.13 0 0 0.77 ± 0.05 0.70 ± 0.22 0.75 ± 0.11 0.90 ± 0.02
  π(%) ± SD 0.09 ± 0.03 0 0 0.24 ± 0.05 0.10 ± 0.04 0.12 ± 0.03 3.60 ± 0.27
  Fu’s F -1.912 - - 0.39 -0.829 -1.039 14.341
  R2 0.1074 - - 0.1439 0.2449 0.1667 0.1461
mtDNA total Sample size/H 17/6 2/2 9/4 8/8 5/5 3/3 44/27***
  Hd ± SD 0.82 ± 0.06 1.00 ± 0.25 0.69 ± 0.15 1.00 ± 0.06 1.00 ± 0.13 1.00 ± 0.27 0.94 ± 0.02***
  π(%) ± SD 0.22 ± 0.05 0.08 ± 0.04 0.32 ± 0.13 0.84 ± 0.15 0.36 ± 0.09 0.21 ± 0.06 8.4 ± 0.61
  Fu’s F 0.347 - 2.112 -2.011 -1.481 - 10.292
  R2 0.1324 - 0.1797 0.1583 0.2111 - 0.1626
  1. Sample size and diversity measures for each gene and for each clade. Hd = haplotype diversity, Gd = gene diversity, H = number of haplotypes or genotypes, π = nucleotide diversity in %, SD = standard deviation. Results of Rozas-Onsins’s (R2) and Fu’s F neutrality tests were recorded for the combined mitochondrial and the nuclear datasets. NW = Ecuador, Costa Rica, Mexico, Texas; CO = Colombia; NE = French Guiana + Venzuela + Brazil (Rondonia); EA = Brazil (Atlantic Coast, Mato Grosso) + Argentina (Yungas); AR = Agentina + Paraguay (Chaco); PE = Perú. *** = p < 0.001. 12SrDNA = small mitochondrial ribosmal subunit, COII = cytochrome oxidase c subunit II, DL = control region or d-loop, ITS2 = intergenic spacer 2, mtDNA = concatenated mitochondrial genes.