The species reference tree of the Bacillus species under study based on 34 concatenated essential genes. The Newick format of this tree is (((Bpu,(Bli,(Bam,(Bmo,Bsu)))),(Bwe,(Bce,Ban,Bth))),(Bha,Bcl)). The evolutionary history was inferred using the neighbor-joining method. The optimal tree with the sum of branch length = 1.58 is shown. The percentages of replicate trees in which the associated taxa clustered together in the bootstrap test (1000 replicates) are shown next to the branches. The tree is drawn to scale, with branch lengths in the same units as those of the evolutionary distances used to infer the phylogenetic tree. The evolutionary distances were computed using the JTT matrix-based method and are in the units of the number of amino acid substitutions per site. All positions containing gaps and missing data were eliminated from the dataset. Phylogenetic analyses were conducted in MEGA4.