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Table 1 Spectral absorbance characteristics for wild type C. nuchalis SWS1 pigments, site-directed mutants, and ancestral pigments

From: Functional characterization of spectral tuning mechanisms in the great bowerbird short-wavelength sensitive visual pigment (SWS1), and the origins of UV/violet vision in passerines and parrots

Pigment

λmaxa(nm)

Shift fromC. nuchaliswt pigmentb(nm)

GBS1 wt

402.97 ± 0.22

 

S90C

363.05 ± 0.05

-40

C86S

403.05 ± 0.12

0

C86Fc

365.72 ± 1.10

-37

C86S/S90C

362.95 ± 0.26

-40

Parrot/Passerine Ancestor

402.93 ± 0.28

0

Passerine Ancestor

404.28 ± 0.28

0

  1. a λmax values are given as mean ± standard deviation from at least three different measurements of dark absorbance spectra per expression. b λmax shifts from C. nuchalis wild type (wt) pigment are expressed as negative for blue shifts. c λmax of single mutant C86F calculated from fitting difference spectra of dark and acid denatured species.