Table 5 Characteristics of the PCR primers used in this study
From: Experimental hybridization and backcrossing reveal forces of reproductive isolation in Microbotryum
N | Loci | PCR primers (forward/ reverse) | Tm, °C | Specificity |
|---|---|---|---|---|
1 | STE20-prA1 | GTTCGATTCGGCAGCAT | 58.5 | a1 in M-Sl |
CACGACAGTCCAAGATTCAA | ||||
2 | STE20-prA1 | CGCAGCTCTCACAAATGAGT | 61.1 | a1 in M-Sd |
ATCGTGGTAGCCCAACGATA | ||||
3 | 236 | GGAATCGACCATGCTAGTGG | 60.0 | a2 in M-Sl and M-Sd |
TAGTCGGAAGGTCGCTGAG |