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Table 1 Heterandria formosa collection sites, sample sizes and genetic diversity measures

From: Paleoclimatic modeling and phylogeography of least killifish, Heterandria formosa: insights into Pleistocene expansion-contraction dynamics and evolutionary history of North American Coastal Plain freshwater biota

Population (specimen no. code†)

No.

Locality

Latitude (° N)

Longitude (° W)

N

Cytbhaps.

Study region

Bayou Nezpique (BNE x LA)

1

Bayou Nezpique, south of I-10, ~30 km east of Lake Charles, Cameron Par., LA

30.245

-92.627

2

22

WCP

Lake Pontchartrain (PON x LA)

2

Lake Pontchartrain, LA

30.189

-90.101

1

31

WCP

Bogue Chitto (BOG x LA)

3

Bogue Chitto R. overflow pool along LA Hwy 21 south of Sun, St. Tammany Par., LA

30.630

-89.897

2

30

WCP

Porter's River (POR x LA)

4

Porter's R. at Davis Landing Rd., St. Tammany Par., LA

30.318

-89.728

2

40, 41

WCP

Wolf Creek Swamp (WCMS x )

5

Wolf Cr. swamp at Belle Ferry Rd., Harrison Co., MS

30.387

-89.197

2

42

WCP

Alabama River trib. (ALA x AL)

6

Unnamed trib. to Alabama R. (Bailey Cr. origin), Wilcox Co., AL

31.981

-87.504

2

‒

‒

Five Points (FIV x FL)

7

Unnamed trib. at Five Points, Tate's Hell State Forest, Franklin Co., FL

29.893

-84.760

9

17, 29

FL

Crooked River (CRO x FL)

8

Crooked R. at Tate's Hell State Forest, Franklin Co., FL

29.893

-84.731

10

17

FL

Ochlockonee River (OCH x FL)

9

Ochlockonee R. at Blountstown Hwy, Leon Co., FL

30.388

-84.665

2

1, 37

FL

Womack Creek (WOM x FL)

10

Womack Cr. at Apalachicola National Forest, Franklin Co., FL

30.009

-84.608

9

1, 3, 11-14

FL

Moore Lake (MOO x FL)

11

Moore Lake at Apalachicola National Forest, Leon Co., FL

30.392

-84.407

10

24

FL

Hill Swale (HIL x FL)

12

Hill Swale, just north of Ochlockonee Bay, Wakulla Co., FL

29.100

-84.406

10

25, 26

FL

Trout Pond (TRO x FL)

13

Trout Pond at Apalachicola National Forest, Leon Co., FL

30.335

-84.385

10

3

FL

Cessna Pond (CES x FL)

14

Cessna Pond at Apalachicola National Forest, Leon Co., FL

30.374

-84.360

9

3, 28

FL

Little Lake (LIT x FL)

15

Little Lake Jackson, west of Hwy 27, northwest Tallahassee, Leon Co., FL

30.526

-84.359

6

2

FL

Wakulla Springs (WAK x FL)

16

Wakulla Springs at Edward Ball Wakulla Springs State Park, Wakulla Co., FL

30.235

-84.302

10

7, 9-10, 20

FL

Lake Iamonia (IAM x FL)

17

Lake Iamonia at CR 155, Leon Co., FL

30.627

-84.291

10

1

FL

Shepherd Spring (SHE x FL)

18

Shepherd Spring at St. Marks National Wildlife Refuge, Wakulla Co., FL

30.123

-84.281

10

9-10, 27

FL

McBride Slough (MCB x FL)

19

McBride Slough at Bloxham Cutoff Rd., Wakulla Co., FL

30.239

-84.270

12

9-10, 43-44

FL

Lake Overstreet (LOV x FL)

20

Lake Overstreet at Alfred B. Maclay Gardens, Leon Co., FL

30.528

-84.257

10

1, 23

FL

Newport Sulphur Spring (NEW x FL)

21

Newport Sulphur Spring, Wakulla Co., FL

30.207

-84.179

10

2, 9, 15, 18

FL

Natural Bridge (NAT x FL)

22

Natural Bridge at Natural Bridge Rd., Wakulla Co., FL

30.284

-84.152

9

2, 18-19

FL

Gambo Bayou (GAM x FL)

23

Gambo Bayou at Lighthouse Rd. west of Stony Bayou Pool, Wakulla Co., FL

30.122

-84.148

9

9, 15

FL

Tram Road (TRA x FL)

24

Tram Rd., 2 km east of Plum Orchard, Wakulla Co., FL

30.140

-84.121

9

9, 15-16

FL

Wacissa River (WAC x FL)

25

Wacissa R., just north of Aucilla Wildlife Management Area, Jefferson Co., FL

30.341

-83.992

10

5-8

FL

Buggs Creek borrow pit (BUG x FL)

26

Buggs Creek barrow pit, FL

30.477

-83.844

2

35-36

FL

Wolf Creek (WOL x FL)

27

Wolf Creek at US 90, Jefferson Co., FL

30.532

-83.812

1

39

FL

Mckey Park (MCK x GA)

28

Mckey Park, Loundes Co., GA

30.864

-83.290

2

45

FL

Bevil Creek (BEV x GA)

29

Bevil Creek at Browns pond Loch Laurel Rd., GA

30.720

-83.244

2

3, 38

FL

Robinson Creek (ROB x FL)

30

Robinson Cr. at CR 131, Columbia Co., FL

30.321

-82.662

3

22

FL

Ichetucknee blue hole (ICH x FL)

31

Blue hole, Ichetucknee State Park, Columbia Co., FL

29.980

-82.758

2

32-33

FL

Hillsborough River (HIR x FL)

32

Hillsborough R. at Tampa, Hillsborough Co., FL

27.957

-82.465

11

22, 47

FL

River Styx (RIS x FL)

33

River Styx at CR 346, Alachua Co., FL

29.517

-82.222

2

‒

FL

Saint Johns River (SJR2 x )

34

Unnamed trib. of the St. Johns R., FL

‒

‒

3

22

FL

Newnan's Lake (NEWn x FL)

35

Newnan's Lake at southeast 16th Ave. boat ramp, Alachua Co., FL

29.637

-82.200

1

3

FL

Everglades (EVE x FL)

36

Everglades drainage, Dade Co., FL

25.552

-80.564

1

30

FL

Bahama Swamp (BAH x SC)

37

Bahama Swamp off Kato Bay Rd., Jasper Co., SC

32.356

-81.044

2

34

ACP

Edisto River (CRO x SC)

38

Edisto River, SC

33.085

-80.603

2

4, 46

ACP

Back River (BAC x SC)

39

Back R. at SSR 503, east of Goose Creek, edge of US Navy station, SC

32.967

-79.938

2

22

ACP

Cooper River trib. (WAD1 x SC)

40

Trib. to Cooper River at Wadboo, Berkeley Co., SC

33.201

-79.927

1

‒

ACP

Waccamaw River (WAR1 x SC)

41

Waccamaw River, SC

33.474

-79.188

1

‒

ACP

Trib. to Waccamaw River (WAR2 x SC)

42

Unnamed trib. to Waccamaw River, SC

33.506

-79.181

1

4

ACP

Lumber River (LUM x SC)

43

Lumber River, SC

33.661

-79.153

1

4

ACP

Cane Branch (CAN x SC)

44

Cane Branch on farm road at Francis Marion National Forest, Berkeley Co., SC

33.185

-79.527

1

21

ACP

  1. Site names (numbers, localities, geographical coordinates), sample sizes (N), regional groups (Figure 1), and cytb haplotypes are indicated. Abbreviations: AL Alabama, Ave avenue, Co County, Cr Creek, CR County Road, FL Florida, GA Georgia, Hwy highway, MS Mississippi, Par Parish, R River, Rd Road, SC South Carolina, Trib tributary.
  2. †x-variables in codes range 1-12, representing each individual sequenced for this study.