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Table 3 Programs used to estimate genetic parameters and conduct statistical tests and procedures

From: A hybrid zone between Bathymodiolusmussel lineages from eastern Pacific hydrothermal vents

Program

Version

Parameters1, tests and embedded procedures

References

Arlequin

3.5.1.3

Diversity indices (H, k, h, π, F ST , φ ST )

[40]

  

Fu’s Fs test

[41]

  

Linkage disequilibrium (D, D’, R2)

[42]

  

IBD: Pairwise Φ ST , F ST and

[43]

  

Mantel test

[44]

Beagle

 

Recombination test

[45]

Beast

1.7.2

Estimation of divergence time

[46]

CNDm

 

Cytonuclear disequilibrium (D, D’)

[47]

CodonCode Aligner

3.7.1.1

Proofreading sequences

CodonCode Corp., Dedham, MA

Genepop

4.0.10

Multilocus data management

[48]

  

HWE exact tests

[49]

IMa2

8.26.11

Demographic parameters (τ, θA, θ1, θ2, m1, m2)

[50]

Geneious

5.6.2

Sequence editing

[51]

Network Publisher

1.3.0.0

Illustrating gene networks

[52]

NewHybrids

1

Assignment of putative hybrids

[53]

Phase

2.1.1

Decomposing heterozygotes, recombination test

[54, 55]

Structure

2.3.3

Assignment test (K)

[56, 57]

  1. 1 Parameters: H, number of haplotypes; k, number of polymorphic sites; h, haplotype diversity; π, nucleotide diversity per site; F ST , standardized molecular variance among populations; φ ST , standardized molecular variance among populations; D, nuclear linkage disequilibrium (LD); D’, standardized LD; R2, correlation measure of LD; estimators of nuclear linkage disequilibrium; τ, time to most recent common ancestor; θA, θ1, θ2, sizes of ancestral and descendant populations; m1, m2, immigration into descendant populations; K, number of genotypic clusters.