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Table 2 PCR primers and methods for six gene loci in Bathymodiolus mussels

From: A hybrid zone between Bathymodiolusmussel lineages from eastern Pacific hydrothermal vents

Locus

Product

Primers

Ref.1

Methods

Length

COI

Cytochrome-c-oxidase subunit-I

COIG/H

[37]

2

570

SAHH

S-adenosylhomocysteine hydrolase

BatSAHHF/370R

[25]3

Fast PCR

~400

ANT

Adenine nucleotide (ADP/ATP) translocase

AntF/AntR1 and AntF/AntR2

[38]

TD and fast PCR

496

Cat

Catchin

CatF/Cat2R

4

Fast PCR5

~500

Col-1

Collagen type XIV

Col160F/Col619R

6

Fast PCR

~430

EF1α

Elongation Factor 1α

BatEf1αF/BatEf1αR

[25]

Fast PCR

540

  1. 1 References in Literature Cited.
  2. 2 PCR program: 95°C/10 min; 35 x [94°C/1 min, 55°C/1 min; 72°C/2 min], extension at 72°C/7 min.
  3. 3 New to this study: Based on [25], SAHH370R, 5'-CGYTCGAACTCGCAAYGTYAGTGG-3'.
  4. 4 New to this study: CatF, 5'-GAGYGTCTTTCMAAGATCATCTCC-3'/Cat2R, 5'-CATTTYCTGATGTTWCGCTGGAT-3'.
  5. 5 Touchdown and Fast PCR: Amplitaq Gold Fast PCR Master Mix, UP (Life Technologies Corp., Carlsbad, CA) and the protocol for the taq supplied by manufacturer of Veriti thermal cycler with an annealing temperature at 50°C (Life Technologies Corp., Carlsbad, CA).
  6. 6 New to this study: Based on [25], Col160F, 5'-GGTTCACGAYCGGAWGTTCCCC-3', Col619R, 5'-TCCCAGCCTTTTCTCGCCCA-3'.