- Research article
- Open Access
Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique
BMC Evolutionary Biology volume 13, Article number: 196 (2013)
Intraspecific genetic variation of African fauna has been significantly affected by pronounced climatic fluctuations in Plio-Pleistocene, but, with the exception of large mammals, very limited empirical data on diversity of natural populations are available for savanna-dwelling animals. Nothobranchius furzeri is an annual fish from south-eastern Africa, inhabiting discrete temporary savannah pools outside main river alluvia. Their dispersal is limited and population processes affecting its genetic structure are likely a combination of those affecting terrestrial and aquatic taxa. N. furzeri is a model taxon in ageing research and several populations of known geographical origin are used in laboratory studies. Here, we analysed the genetic structure, diversity, historical demography and temporal patterns of divergence in natural populations of N. furzeri across its entire distribution range.
Genetic structure and historical demography of N. furzeri were analysed using a combination of mitochondrial (partial cytochrome b sequences, 687 bp) and nuclear (13 microsatellites) markers in 693 fish from 36 populations. Genetic markers consistently demonstrated strong population structuring and suggested two main genetic groups associated with river basins. The split was dated to the Pliocene (>2 Mya). The northern group inhabits savannah pools across the basin of the intermittent river Chefu in south-western Mozambique and eastern Zimbabwe. The southern group (from southernmost Mozambique) is subdivided, with the River Limpopo forming a barrier (maximum divergence time 1 Mya). A strong habitat fragmentation (isolated temporary pools) is reflected in significant genetic structuring even between adjacent pools, with a major influence of genetic drift and significant isolation-by-distance. Analysis of historical demography revealed that the expansion of both groups is ongoing, supported by frequent founder effects in marginal parts of the range and evidence of secondary contact between Chefu and Limpopo populations.
We demonstrated: (1) ancient (pre-Pleistocene) divergence between the two main N. furzeri lineages, their recent secondary contact and lack of reproductive isolation; (2) important genetic structuring attributed to the fragmented nature of their environment and isolation-by-distance, suggesting that dispersal is limited, occurs over short distances and is not directly associated with river routes; (3) an apparent role of the River Limpopo as a barrier to dispersal and gene flow.
Phylogeography and historical demography play crucial roles in explaining species distributions and are instrumental in revealing episodes of fragmentation and subsequent expansions connected to environmental and climatic fluctuations [1, 2]. Most phylogeographic research has been focused on the Northern Hemisphere, often linked to the Pleistocene glacial cycles. Our knowledge of biogeographical patterns and processes in the Southern Hemisphere is relatively poor , and it is especially marked in non-forest biomes. In sub-Saharan Africa studies on large mammals living in savannah have identified large-scale phylogeographic patterns and provided a basic understanding of the major biogeographic regions at a continental scale , though studies with small-scale resolution are still rare [5–8].
In Africa, Pleistocene climatic fluctuations have resulted in more arid conditions during glacial periods, as large masses of water were deposited in continental glaciers, alternating with more mesic conditions during interglacials (termed pluvials in the tropics). Thus African ecosystems have undergone a series of aridification events, accompanied by spreading savannah habitats, since the Pliocene, with relatively wetter periods between 2.7-2.5 Mya, 1.9-1.7 Mya and 1.1-0.9 Mya . Further aridification is documented in the late Pleistocene and, notably, the last interpluvial (130–114 kya) was coincident with a series of extreme droughts in eastern Africa . Studies on large savannah-dwelling mammals demonstrated their less pronounced phylogeographic structure and high level of genetic variation in Southern Africa, suggesting a long-standing persistence of large savannahs in this part of the continent (reviewed in ). However, research from temperate areas in the northern latitudes led to understanding that large and highly mobile animals are not ideal taxa to specify the location and character of past refugia (e.g. ). On the contrary, species with very low dispersal ability and strong dependence on particular habitat have provided much more detailed picture of phylogeographical patterns and processes, for example the identification of unexpected cryptic refugia (e.g. ) or “refugia within refugia” (e.g. [8, 13]).
Killifishes (Cyprinodontiformes) are an ancient fish clade with a predominantly Gondwanan distribution and high species richness in Africa . African killifish (family Nothobranchiidae) include several clades that show radiations in specific habitat conditions, such as small rain forest streams, margins of large rivers or brackish zones . A notable clade, comprising the genus Nothobranchius (59 species; ) from East Africa and related monotypic genera Pronothobranchius and Fundulosoma from West Africa, has adapted to a unique environment of temporary savannah pools . The pools inhabited by Nothobranchius are completely separated from river systems and typically not inhabited by other fishes except lungfish (Protopterus spp.). The pools are strictly seasonal and filled with rainwater at the beginning of the rainy season. Pools desiccate during the dry season, with the duration of pools depending on local climate and ranging from 3–11 months [15, 17, 18]. In some years, a major flooding may connect several pools via flooded savannah and may also connect pools with intermittent streams, though such events appear to be rare. Importantly, specific soil conditions (the presence of alluvial vertisols) are necessary for the occurrence of Nothobranchius [17, 19], limiting dispersal and occurrence of Nothobranchius across the savannah to specific habitat patches. Therefore, population processes affecting genetic structure of Nothobranchius are predicted to be a combination of processes that are unique to Nothobranchius and these affecting terrestrial and aquatic taxa.
All Nothobranchius species are annual. Fish hatch soon after a pool fills with water [15, 20], grow rapidly, achieve sexual maturity within a few weeks , and reproduce daily thereafter . Adult lifespan is limited by habitat desiccation, with the following generation surviving in the form of diapaused embryos in desiccation-resistant egg envelopes encased in dry mud . Populations of Nothobranchius seldom co-occur with other teleost fish species . Dispersal by Nothobranchius may be limited to occasional large-scale floods in years with unusually high rainfall, which may transport live fish among savannah pools. Alternatively, eggs encased in mud may be carried attached to the bodies of large herbivores, as reported for aquatic invertebrates and macrophytes , or possibly by waterbirds.
Here we use Nothobranchius furzeri Jubb to investigate phylogeographic patterns and demographic processes in this group of annual, savannah-adapted fish. Its rapid development, short lifespan and age-dependent deterioration of physiological functions have made it a valuable vertebrate model organism in ageing research . In contrast to that, little is known about natural populations . Nothobranchius furzeri is distributed in southern Mozambique , with a single locality known in Zimbabwe (Sazale Pan at Gona Re Zhou National Park, Chefu basin, close to the Mozambican border) . Within its geographical range this species is restricted to pools associated with vertisol soils, while it is absent from pools associated with sandy and lateritic soils [17, 19]. Previous research on the phylogenetic relationships of Mozambican Nothobranchius included samples from several N. furzeri populations . The mitochondrial marker COI (cytochrome oxidase I) suggested high spatial structuring within the range of all species investigated, but the three nuclear sequences used in the study (GHITM, Cx32.2, PNP) had insufficient power to resolve intraspecific relationships.
Here, we provide the first detailed study of the phylogeographic patterns and demographic processes in N. furzeri, covering the entire distribution range of the species using a combination of mitochondrial gene CYTB (cytochrome b) and 13 nuclear microsatellite markers. Specifically, we tested following hypotheses: (1) N. furzeri is composed of three reproductively isolated clades (sensu ), with no secondary contact among them. (2) The main divergence was influenced by Plio-Pleistocene climate changes with allopatric diversification due to savannah fragmentation during pluvials. (3) The populations of savannah-dwelling Nothobranchius fishes have expanded during last interpluvial, together with their preferred habitat. (4) Genetic diversity will be lower and genetic structure higher on the range periphery, where populations are exposed to more frequent extinction-recolonization processes associated with strong genetic drift.
Fish were collected during 8 field trips between 2008 and 2012, visiting 336 individual savannah pools across southern and central Mozambique, with the aim of sampling pools across the entire savannah habitat in that region. From a total of 163 pools populated by Nothobranchius spp., 73 pools were inhabited by N. furzeri, all of them south of the River Save (Figure 1). Fish were collected using dip and Seine nets. Species identification took place in the field, and at most sites a small fin clip from the caudal fin was taken and stored in 96% ethanol. Only adult fish, clearly identified to species, were sampled. Each population sample consisted of fish collected the same day. Different populations (within and across geographical regions), however, were collected across study years (Table 1). There was no major flood between 2008 and 2012 that would cause a dispersal of fish or eggs across populations. A subsample of captured fish was stored in 5% formaldehyde or 80% ethanol for further reference and phenotype analyses while most fish were released back into the pool. Two GRZ (type locality) specimens were obtained from the breeding stock in Leibniz Institute for Age Research, Jena, Germany. For genetic analysis, N. furzeri populations from 36 pools (= localities) were selected which cover the entire known species range (Table 1, Figure 1). Adjacent savannah pools were generally omitted, except for two regions where analyses focussed on small-scale structuring. All fieldwork complied with legal regulations of Mozambique (collection permit 154/II/2009/DARPPE and sample export permit 049MP00518-A/09 of the Mozambican Ministry of Fisheries).
Genotyping of microsatellites and mtDNA
In total, 673 individuals collected at 31 localities (Table 1) were genotyped at 13 microsatellite loci in four multiplex PCR sets. PCR products were separated on the ABI Prism® 3130 Genetic Analyzer (Applied Biosystems) and analysed using GeneMapper® v. 3.7 (Applied Biosystems) (see Additional file 1 for more details). Partial mitochondrial gene CYTB was amplified using primers FW40 (GCA AAT GAC TCC CTA ATT GAC C) and REV1019 (CCT CCA ATT CAT GTT AGG GTG) designed on the basis of the mtDNA sequence of N. furzeri (GenBank Accession NC_011814). PCR products were sequenced directly using BigDye Terminators v. 3.1 chemistry (Applied Biosystems) (see Additional file 1 for more details).
Analysis of population genetic structure in the distribution range of N. furzeri
Network analysis of mtDNA variation
Sequence variation in CYTB (687 bp fragment in 149 individuals from 36 populations; Table 1) was visualised as a haplotype network using the median-joining algorithm in Network 4.610 . All sequences were geo-referenced and the distribution of the main genetic groups was plotted onto map using PanMap software (http://www.pangaea.de/software/PanMap).
FST and STRUCTURE - microsatellite data
We estimated the proportion of null alleles (NA) at each locus and population in FREENA . Linkage disequilibrium among 13 polymorphic loci was examined in each population with exact tests using Markov chain methods in GENEPOP 3.4 . Corrections for multiple testing were performed using the false discovery rate (FDR) approach. Genetic differentiation between sampling sites was quantified by computing pairwise estimators of FST according to  and their significance was tested by 1000 permutations in GENETIX 4.03 . For all analyses based on allele frequencies in populations (like FST), two genotyped individuals from captive population GRZ were removed.
An individual-based Bayesian clustering procedure, implemented in STRUCTURE 2.3.3 , was used for individual-based assignments to distinct genetic clusters. The Bayesian model assumes K (unknown) populations with different allele frequencies at a set of independent loci. The program was run with 20 independent simulations for each of K from 1 to 15, each of 106 iterations, following a burn-in period of 105 iterations. In all simulations an admixture ancestry model and correlated allele frequency model (with λ = 1) was used. The likelihood of K; i.e. Ln Pr(X|K), was used to infer the best number of real populations in the datasets using the method of Evanno et al. . We also forced the assignments of individuals to clusters beyond the number considered to maximize the posterior probability of the data. This approach was used to reconstruct the hierarchical relationships among populations, as well as to distinguish between historical processes that are likely to shape the structure (e.g. ). The results of 20 replicate runs for each value of K were combined using the Greedy algorithm of CLUMPP 1.1.1  and summary outputs for each value of K were displayed graphically using DISTRUCT v. 1.1 .
Spatial genetics - microsatellite data
An individual-based spatial approach of Bayesian clustering was used to analyze the spatial structure of the genetic data. We employed the methods implemented in BAPS version 4.1 . BAPS estimates the hidden population substructure by clustering individuals into genetically distinguishable groups based on allele frequencies and linkage disequilibrium. A major advantage compared to most other methods is that the number of populations is treated as an unknown parameter that can be estimated from the dataset. We performed 20 independent runs examining the spatial clustering of groups of individuals (collected at the same locality). Based on the initial results, we tested specific hypotheses by repeating the analyses for a fixed number of populations (i.e. fixed-K spatial clustering of groups of individuals).
Isolation by distance was analysed by regressing pairwise estimates of FST/(1- FST) against ln-distance between sample sites . Mantel tests were used to test the correlation between matrices of genetic differentiation and Euclidean distances between sampling sites by 1000 permutations in GENEPOP. This test was performed for the complete dataset (excluding only two individuals from captive population GRZ) and separately for the three groups of populations defined by preliminary analyses of mtDNA and microsatellite data (i.e. Chefu, LimpN, LimpS; Pop406 was excluded, because it concerns the secondary contact of two differentiated groups - see below; see Table 1 for assignment of populations to particular groups).
To test particular hypotheses of the genetic connectivity among populations, we used the maximum likelihood approach to estimate the effective population numbers and immigration rates as implemented in Migrate v. 3.5.1 . We performed the Likelihood ratio test (LRT) to test the fit of a priori defined scenarios of the gene flow against the full model, assuming n-island migration model. In particular, we considered two migration models. First, we assumed that migration only occurs between neighbouring populations along the axis following the course of the main rivers (stepping-stone migration model). Second, we assumed that migration only occurs downstream, i.e. from a higher altitude sites to the lower altitude sites (mediated by floods).
Due to high number of sampling sites in the Chefu region, which would make the model computationally too complex, we performed the Migrate analysis only in the LimpS and LimpN clades (Table 1, Figure 1). We were forced to further omit several sites due to their admixture (Pop406; see below) and position outside the main river axis, given that they impeded the definition of migration scenarios and increased the number of degrees of freedom of the model. Hence, the migration matrix was only evaluated for populations 207, 120, 119 and 326 in the LimpS clade and 13, 8, 2, 1, 50 and 55 in the LimpN clade; the rank representing upstream to downstream linear transects. Migrate was initially run with 10 short and 4 long chains of default settings. Subsequently, the results were used as starting values for extended runs with 10 short chains of 1000 sampled genealogies with 100 increment and 4 long chains of chains of 10000 recorded steps and 100 increment value and a mixing scheme with temperatures ranging from boiling to cool (106, 3, 1.5 and 1) and swapping interval of 1. We monitored if independent runs provided consistent results and in the final runs, defined migration scenarios were used as alternative hypotheses for the LRTs.
Intrapopulation genetic variation and historical demography
Genetic variation and bottleneck - microsatellites
Deviations from Hardy-Weinberg equilibrium (HWE) were tested for each locus and population using the Markov chain method in GENEPOP. The number of alleles (A), observed (HO) and expected (HE, non-biased estimate) heterozygosities were calculated in GENETIX. Allelic richness (AR) corrected for sample size by the rarefaction method (i.e. estimated for a minimum sample size of 11 diploid individuals) were calculated for each population in FSTAT 126.96.36.199 .
Excess heterozygosity with respect to that expected at mutation-drift equilibrium for the number of alleles present may indicate a genetic bottleneck . We estimated the deviation of gene diversity from mutation-drift equilibrium at the local scale, using BOTTLENECK 1.2.02 . We used a generalized mutation model in which the change in the number of repeat units forms a geometric random variable, with a variance of the geometric distribution fixed to 0.36, which is likely to be the most appropriate value for microsatellites . This estimate was simulated using 1000 permutations in BOTTLENECK by choosing the two-phase mutation model (TPM), with a variance for TPM equal to 0.36 and a proportion of the stepwise mutation model in TPM equal to 0.7. The significance of deviation from the expected heterozygosity under mutation-drift equilibrium was determined with a one-tailed Wilcoxon signed rank test  and the results were corrected for multiple tests using the FDR approach (see above).
Historical demography based on mtDNA
Demographic analyses assume more or less homogeneous populations. Based on preliminary analyses of microsatellites in STRUCTURE and BAPS as well as the haplotype network of mtDNA and distribution of haplotypes, we defined three genetic groups (= populations; see Table 1 for assignment of individuals into the groups). For analysis of historical demography, we used the reduced dataset of 132 longer mtDNA sequences (815 bp) from individuals collected at 32 localities (Table 1). We also excluded 6 individuals from Pop406 (secondary contact of two differentiated lineages - see below) and a sequence from GRZ (laboratory population). Diversity estimates, i.e. number of polymorphic sites (Np), number of haplotypes (Nh), haplotype diversity (Hd), nucleotide diversity (Pi, expressed as percentages, i.e. 0.001 = 0.1%), the average number of nucleotide differences (k) and Watterson’s estimate of θ (θ = 4Ne*μ) were calculated using DnaSP v. 5.10.01 .
Demographic histories of the three groups were evaluated using several approaches. We first estimated the neutrality indices Tajima’s D and Fu’s Fs and Ramos-Onsins and Rozas’s R2 statistic  in DnaSP. They are sensitive to population expansion, contraction and structure and return significantly negative (D, Fs) or small (R2) values in the case of recent population expansion, while population decline and/or structure tend to return positive or high values.
Second, we used the DnaSP and ARLEQUIN 3.11  to reconstruct the histogram of pairwise differences in each clade (mismatch distribution; MD) to test for non-random distribution of coalescence events in the sample. ARLEQUIN was used to test the fit of our datasets to the expectations of the sudden demographic expansion model using the sum of squared deviations between the observed and expected mismatch from 1,000 parametric bootstrap replicates. Under the assumption of the (sudden) demographic expansion model the MD also permits estimation of the time of onset of population expansion τ (τ = 2 t* μ; t = time in years, μ = mutation rate per locus of 815 bp). Parameter τ was estimated with DnaSP using the moment method of Rogers  assuming the infinite sites model (IFM) as well as with ARLEQUIN using Schneider & Excoffier’s method , which relaxes the IFM assumption. Confidence intervals were obtained by a parametric bootstrap approach based on 1000 replicates performed in ARLEQUIN.
Third, we reconstructed demographic history of the main lineages backward in time using the coalescent-based Bayesian skyline plot (BSP)  in BEAST, which infers past population size changes from sequence data . Analyses were run twice for each lineage using the HKY model of sequence evolution, which was an appropriate model within each lineage selected with the jModelTest  under the AIC criterion. The MCMC simulations were run with 50 million iterations with a sampling increment of 300 and results were checked for convergence and stationarity of different runs in Tracer 1.4.1 and combined in LogCombiner 1.4.8 module.
Dating of divergence
To estimate the divergence times of the major phylogroups we reconstructed the time-calibrated tree in BEAST using the dominant (putatively ancestral) haplotypes from each clade and single sequence of closely related species N. kadleci, N. orthonotus and N. rachovii. The tree was rooted with Aphyosemion herzogi (GenBank: EU885235.1). An appropriate model of sequence evolution (TrN + I; I = 0.63) was selected with the jModelTest  and implemented in BEAST with Jeffreys prior for substitution rates. Divergence times were estimated under a Yule branching process with a constant speciation rate as a tree prior and assuming an uncorrelated lognormal relaxed molecular clock . A normally distributed prior with a mean of 0.007 and standard deviation of 0.0010 was used for the mean mutation rate, since it covered the plausible range of 0.70–0.86% substitutions per site per My, as discussed in . The search started with a UPGMA tree and two independent runs of 3×107 generations were conducted with sampling every 1000th generation. The results were checked for convergence and stationarity using Tracer and combined in LogCombiner after a burn-in of the first 10% of generations. Finally, the molecular clock tree was summarised using the TreeAnnotator 1.4.8 module, using the medians as node heights. Given the lack of an internal calibration point, divergence time estimates are likely overestimates of the real time due to the tendency of τ to artificially antedate recent events .
Genetic diversity of local populations
The mean frequency of microsatellite null alleles per population was less than 5% for all but one locus. However, even for this locus (Nfu_0140_FLI) the mean frequency of null alleles was low (5.25 ± 5.24%) and we did not consider null alleles as a problem in subsequent population genetic analyses (but see further for their possible effect in tests of the HWE). The microsatellite loci used in our study can be considered to be unlinked. From 1841 results of genotypic linkage disequilibrium tests (i.e. with adequate data), only 50 were significant at p < 0.05. Most pairs of loci were significantly linked only in one or two populations. Only two pairs showed significant linkage in four (out of 30) populations (Nfu_0023_FLI - Nfu_0038_FLI and Nfu_0010_FLI - Nfu_0041_FLI), which is consistent with their position in the same linkage group (Additional file 1), i.e. exhibiting an inter-marker distance of 7 and 5 cM in N. furzeri GRZ strain .
Most populations (25 out of 30) showed deviance from HWE, when calculated over all loci. In most cases deviations from HWE were caused by null alleles present at an increased frequency at some loci and populations. The mean frequency of null alleles for 21 locus-population tests significant after FDR correction (from 13 × 30 = 390 locus-population pairs) was 13.1 ± 5.45% (N = 21), while for the whole dataset the mean frequency of null alleles was only 2.43 ± 4.14% (N = 390). In most populations, the HW disequilibrium was caused by just one or two loci. The only exceptions were peripheral populations Pop124 and Pop323, which showed a deficit of heterozygotes at four loci, suggesting an increased level of inbreeding (Figure 2).
All measures of intrapopulation genetic variation (HO, HE, AR; Table 2) were strongly correlated (all R > 0.8, p < 0.05) and we describe only the distribution of AR. The range of AR was 4.85-13.50 (rarefaction estimate for the lowest sample size N = 11). The most variable populations were usually located in the centres of the distribution of the three main genetic groups. The lowest estimates of AR were encountered in sites at the periphery of their distribution (Figure 2, Table 2). These local populations (e.g. Pop13, Pop323, and Pop422) are likely to represent recent founder effects, supported by the results of Bottleneck tests, where the only population with a significant heterozygosity excess (after FDR correction) was Pop13, indicating recent decrease in effective population size (Table 2). The diversity of mtDNA at these localities was also low with the only haplotype at Pop13 (n = 3) and two haplotypes differing by 1 bp substitution at Pop323 (n = 7) and Pop422 (n = 3).
Spatial genetic structure over the entire distribution range of the species
Median-joining network analyses of 149 CYTB sequences (687 bp) revealed 49 different haplotypes from two main genetic groups, likely associated with discrete river basins (Figures 1 and 3). The first, northern group was found to inhabit savannah pools across the basin of the intermittent River Chefu. Within this haplogroup, three haplotypes (h3, h4, h20) formed a separate lineage, distributed in the easternmost region of the N. furzeri distribution (Figure 3). The second, southern group was clearly subdivided into northern (LimpN) and southern (LimpS) subgroups, with the River Limpopo representing possible barrier. The LimpS group contains pools located within the basins of the Limpopo and Incomati rivers. Population 406 likely represents an example of secondary contact between the Chefu and LimpN mtDNA haplogroups. From six individuals, two had haplotype h24, which is frequent in LimpN populations. However, four individuals expressed private haplotypes for this population, h16 and h17, which belong to the Chefu haplogroup (Figure 3). Sequencing a shorter fragment (481 bp) of CYTB in nine additional individuals (not included in any other analyses) revealed the same proportion of Chefu and LimpN haplotypes; six individuals possessed Chefu haplotypes and three individuals the LimpN haplotypes.
Based on microsatellite data, N. furzeri in the study area is genetically structured (mean pairwise FST = 0.077 ± 0.0451). Only five of 420 pairwise FST were not significant (Additional file 2) and these five pairs were all geographically close to each other in the centres of their respective haplogroup ranges. On the other hand, the three closest populations (Pop53, Pop55, and Pop121 - all of them belonging to LimpN, with a distance from each other only 1.3-2.7 km) were significantly different, with FST values from 0.015 to 0.045. There were even extremely high pairwise FST between neighbouring populations (e.g. Pop215 and Pop323, 48 km distance within the Chefu group: FST = 0.210) suggesting important barriers to gene flow over short distances and/or strong effect of genetic drift.
The most supported division in Bayesian clustering in STRUCTURE was for K = 2, but suitable models according to ΔK are also represented by K = 5, 7, 9, and 13 (Additional file 3). In the model for K = 2, the clustering of populations was congruent with the two main mtDNA haplogroups (i.e. Chefu vs. Limpopo) and, similarly, for K = 3 the populations around the River Limpopo roughly diverged to LimpS and LimpN (Additional file 3). Further increases in the number of presumed clusters led to separation of other populations, most often in peripheral parts of the distribution of the three main genetic groups (see Figure 2 for the situation in K = 7, where the most distant populations in specific groups were assigned to separate clusters). Population 406, with individuals belonging to the two mtDNA haplogroups (Chefu and LimpN), is genetically homogenous on nuclear microsatellites (Additional file 3) suggesting that there is no reproductive isolation between the two mtDNA lineages.
A similar pattern of genetic structure was observed in the spatial analysis in BAPS (Additional file 3). The best model identified 13 genetic populations, which are almost identical to those in STRUCTURE for K = 13 (Additional file 3). The main divergence is between northern (Chefu) and southern (Limpopo) populations (in model K = 2). Further increase in the number of clusters led to separation of peripheral populations in all three main groups and separation of left and right banks of the River Limpopo (LimpN and LimpS) (Additional file 3).
The genetic distance between populations was correlated with their geographic distance, when all populations were analysed together (Mantel test, 1000 permutations, p < 0.001). When populations were separated into the three main genetic groups, the IBD pattern remained significant only in the Chefu populations (p = 0.001), despite the same trend observed in both LimpN (p = 0.062) and LimpS (p = 0.085). This pattern was strongly influenced by the genetic distinctiveness of peripheral populations in all three groups, mainly populations 323, 13, and 124 in Chefu, LimpN, and LimpS, respectively (Additional file 2).
The analysis of genetic connectivity for LimpS and LimpN clades in Migrate is summarized in Table 3. All alternative hypotheses always provided significantly lower fit than the full model (LRT tests; p < 0.001). This demonstrates that genetic connectivity in both clades strongly deviate from a simple stepping-stone migration model and from a model assuming strictly downstream migration. A further support comes from the fact that the full models indicated that all localities received non-negligible immigration rates from non-neighbouring sites and that several localities in both clades received considerably higher immigration rates from sites located at lower altitude than from any of the upstream sites (Table 3).
Historical demography based on mtDNA
Comparison of mtDNA variation among the three main genetic groups and the results of neutrality tests are shown in Table 4. All populations significantly fitted the model of sudden growth according to SSD analysis in ARLEQUIN and showed a unimodal pattern (Figure 4a). Bayesian skyline plots indicated moderate (LimpN, LimpS) to intensive (Chefu) population growth in all three genetic groups (Figure 4b). This finding is consistent with neutrality tests that showed a non-significant signal for population growth in the LimpS and LimpN clades (where only the R2 values were significant), while all tests indicated highly significant deviations from neutrality in the Chefu clade (Table 4). The τ values from DnaSP suggested more recent population expansions compared to estimates by ARLEQUIN, which conforms to a previously reported tendency of the moment method to underestimate the true values . Nonetheless the DnaSP estimates were always included within the 90% CI of ARLEQUIN (Table 4). ARLEQUIN analysis was congruent with the BSP analysis and indicated a more recent population expansion in the Chefu group (mean estimates 70–118 kya according to the method used) compared to two Limpopo groups (112–255 kya) (Table 4). This timing is comparable to that suggested by the BSP analysis (Figure 4b).
Divergence times among dominant haplotypes in clades
Assuming 0.007 substitutions per site per Mya, the clade LimpS diverged from LimpN approximately 0.99 Mya (0.02-8.39), while the Chefu clade diverged from the Limpopo around 3.64 Mya (0.43-19.95). The age of the whole F clade sensu (i.e. N. furzeri + sister species N. kadleci) was estimated at around 6.72 Mya (1.58 – 31.99). Note that the estimated divergence time likely represents the upper limits of the actual timing .
We demonstrated a deep and ancient division of N. furzeri populations into two major clades, likely associated with river basins (northern: Chefu and southern: Limpopo + Incomati). Finer subdivision of the southern clade is facilitated by the River Limpopo, which apparently serves as a barrier to gene flow between the two subclades. Within clades, high genetic structuring of populations inhabiting individual pools likely stems from limited dispersal between pools and frequent founder effects and bottlenecks. Significant isolation by distance within main genetic groups suggests that dispersal occurs in small steps. Genetic connectivity analysis further demonstrated frequent migration to the higher altitude, discounting hypothesis that dispersal is strictly related to major floods associated with river routes. Demographic analyses revealed ongoing expansion of the main clades from their putative refugia.
Historical processes and evidence for two savannah refugia
The existence of two major clades of N. furzeri was unambiguously confirmed by both mitochondrial and nuclear markers. This division suggests a historical split of N. furzeri populations between two refugia. Based on current genetic diversity at microsatellite markers and haplotype distribution, the first refugium was probably located in the central Chefu basin. The second refugium is hypothesised to have been located in the left bank of the Limpopo, in the region between the confluences of the Limpopo with the Rivers Olifantes and Chefu (Figure 2). The estimated divergence time of 3.6 Mya places the divergence event within a period during the Pliocene characterised by a relatively warm and humid climate . Such climatic conditions may have resulted in fragmentation of savannah biome into discrete units separated by widely distributed forests and forested areas may have played an important role in splitting the range of N. furzeri into two refugia. Populations of N. furzeri may have expanded during continuous aridification of the region at the end of Pliocene and during cold and dry periods of Pleistocene, when the open savanna conditions had become more prevalent . In one of these dry periods, the fish from the southern refugium probably colonized the right bank of the River Limpopo, including the pools belonging to Incomati River basin. Interestingly, there are no apparent morphological differences between the isolated clades, except for the relative proportion of male colour morphs , though a rigorous comparative morphological study is lacking.
The River Chefu (sometimes referred to as the River Changane in its lower course) is at present a relatively small river, with a large portion of the river bed dry for much of the year, including an extensive period of the rainy season (notably in its upper and middle reaches). The area forms a large pan confined by hills in the south-west and the River Save basin in the north (Figure 1). To the east the catchment is separated from the Indian Ocean by a series of parallel ridges, effectively forming an enclosed basin and indicating a potential existence of a large lake in mesic periods (Figure 1). While pedological and palynological data are lacking, the existence of grassland (rather than woodland) savannah, flat topography, sandy soils and numerous temporary lakes in that area lead us to speculate that an extensive region of the south-eastern part of the Chefu basin may have been continuously flooded during wetter climatic periods and formed an effective barrier to N. furzeri dispersal.
The distribution of genetic variation suggests further splitting of southern lineage at an estimated time of 1 Mya. Dating of this event corresponds with the last wet period in the African climate record (dated as 1.1-0.9 Mya), followed by a further aridification in late Pleistocene . Well developed riverine forests along Limpopo River may have functioned as an efficient barrier to gene flow for savannah-dwelling animals, as documented for rodents of the genus Aethomys. Thus, unusually for a fish, a large river (the Limpopo) has formed a barrier to gene flow between populations. Rivers, including their margins and active alluvium, represent an inhospitable environment for Nothobranchius and dispersal across the main channel may be an extremely rare event. An analogous pattern has been described for riverine cichlid fish from the lower Congo basin, where powerful rapids in the main river form a strong barrier to dispersal between shallow-water cichlids species, effectively isolating populations on opposite banks of the river .
We identified a single population with secondary contact between the Chefu and Limpopo groups (Figure 1), where mtDNA haplotypes from both haplogroups were unambiguously identified, while nuclear markers revealed no intrapopulation genetic structure (Additional file 3), suggesting a lack of reproductive isolation between the two mtDNA clades. Interestingly, our transect sampling at the other potential contact zone between the two major groups identified a region where group-specific populations occur only 11 km apart (Pop325 and Pop13; Figure 1), with no evidence of secondary contact despite intensive sampling in that region and no clear geographical or geological barrier. Whether this region is only an arbitrary location where distribution of the two clades is bordering or whether it represents putative suture zone (sensu) needs to be confirmed by studies of other taxa. Suture zones, where clades from separate refugia meet, are congruent among taxa in Europe and North America [2, 62] and have also recently been confirmed for savannah-dwelling mammals in Africa, separating eastern and southern savannah regions . It is notable that despite a putative Pliocene divergence (3.6 Mya), there is no reproductive barrier between the two clades, as indicated by microsatellite data for Pop406. In contrast, similarly old divergence events have led to speciation in several taxa in the Northern Hemisphere , murid rodents of Sudanian savannah in western Africa (e.g. [63, 64]), and intralacustrine radiations of cichlid fishes in African lakes ).
Demographic analysis: contemporary processes
Our data indicate that individual N. furzeri populations are subject to strong fluctuations in size, extinctions, and recolonizations, suggesting a strong metapopulation structure over an evolutionary time scale. These conclusions are based on evidence for recent genetic bottlenecks, low allelic richness and Hardy-Weinberg disequilibria, particularly in populations at the periphery of the range. Strong geographical structuring, with significant FST values, even between adjacent populations that are separated by 1.5 km of flat woodland savannah habitat, suggests that dispersal between pools is a rare event on an ecological time scale.
Dispersal of N. furzeri populations across savannah is slow and likely to be accomplished through longer-term geological events at the larger scale. Propagules of a large number of aquatic invertebrates and macrophytes have been reported to be effectively dispersed by large herbivores  or water birds, though a hypothesis for the animal-mediated dispersal of Nothobranchius eggs encased in mud over longer distances appears unlikely given the limited contact between the clades and populations. Similarly, dispersal aided by waterbirds would result in weaker geographical structuring. On the other hand, genetic connectivity analysis clearly demonstrated that dispersal is not unidirectional and propagules (eggs or adults) from the lower altitude sites sometimes colonize higher altitude pools. This may indicate animal-mediated dispersal on short distances. At present, these ambiguous outcomes make Nothobranchius dispersal mode elusive, but a further insight may be provided by ongoing analysis of sympatric congeners.
The Chefu group, with strong isolation by distance, a star-like haplotype network, and decreasing genetic diversity from the centre to the periphery of its range has all the attributes of an expanding population, supported by analysis of its historical demography. This expansion is dated to begin approximately 160–250 kya, corresponding to a very dry phase in the African climate (penultimate glacial/interpluvial), and predating a series of extreme droughts reported from eastern Africa , which resulted in the disappearance of several large lakes such as Lake Victoria . Weaker, but comparable, signals of an expansion indicate a similar pattern for the two Limpopo groups.
Consequences of genetic structure for ageing research
Nothobranchius furzeri represents an emerging model for ageing research and different captive strains of N. furzeri of different geographic origin show large differences in captive lifespan and senescence rates . Our study confirms that populations of N. furzeri are strongly structured and suggests that two large groups represent ancient clades. This finding contrasts with other fish genomic models (three-spined stickleback, cichlid lake radioations) [65, 68] which are genetically less diversified despite their greater morphological variability. The current range of N. furzeri spans a strong cline in environmental conditions. More arid conditions are found in the Chefu river basin where savannah pools are filled with water for a shorter period, while relatively more mesic conditions are found in the southern part of its range [18, 67]. We demonstrated that both main N. furzeri clades have ranges encompassing a substantial variation in environmental conditions, making comparative studies possible at two levels of divergence. As genome sequencing of N. furzeri is underway  and genomic regions controlling simple traits have been mapped by linkage analysis , an intriguing possibility includes comparative genomics aiming at identifying genetic variation associated with different lifespans [56, 68].
Availability of supporting data
Sequences of CYTB of 149 individuals of N. furzeri were submitted to GenBank (Accession numbers KC777039-KC777187), and microsatellite dataset was deposited to the database DRYAD (doi:10.5061/dryad.30sq1).
In this study we provide the first detailed analysis of the phylogeographic patterns and demographic processes in a short-living fish N. furzeri, a newly emerging model in aging research. By using the comprehensive sampling covering the entire distribution range of the species and by a combination of mitochondrial and nuclear genetic markers we demonstrated: (1) ancient (pre-Pleistocene) divergence between the two main N. furzeri lineages, their recent secondary contact and lack of reproductive isolation; (2) important genetic structuring attributed to the fragmented nature of their environment and isolation-by distance, suggesting that dispersal is limited, occurs over short distances and is not directly associated with river routes; (3) an apparent role of the River Limpopo as a barrier to dispersal and gene flow.
This is a part of master thesis of VB, supervised by JB and MR. They are all interested in evolutionary processes influencing African biodiversity using fish and mammals as model animal groups. MP and RB are working in MR’s lab on the factors affecting life histories of Nothobranchius fishes, the research of AC and KR is focused on aging processes using killifishes as one of the models.
Dynesius M, Jansson R: Evolutionary consequences of changes in species’ geographical distributions driven by Milankovitch climate oscillations. P Natl Acad Sci USA. 2000, 97: 9115-9120.
Hewitt G: The genetic legacy of the quaternary ice ages. Nature. 2000, 405: 907-913.
Beheregaray LB: Twenty years of phylogeography: the state of the field and the challenges for the southern hemisphere. Mol Ecol. 2008, 17: 3754-3774.
Lorenzen ED, Heller R, Siegismund HR: Comparative phylogeography of African savannah ungulates. Mol Ecol. 2012, 21: 3656-3670.
Smit HA, Robinson TJ, Van Vuuren BJ: Coalescence methods reveal the impact of vicariance on the spatial genetic structure of Elephantulus edwardii (Afrotheria, macroscelidea). Mol Ecol. 2007, 16: 2680-2692.
Russo IRM, Chimimba CT, Bloomer P: Bioregion heterogeneity correlates with extensive mitochondrial DNA diversity in the namaqua rock mouse, Micaelamys namaquensis (Rodentia: Muridae) from southern Africa - evidence for a species complex. BMC Evol Biol. 2010, 10: article 307
Willows-Munro S, Matthee CA: Linking lineage diversification to climate and habitat heterogeneity: phylogeography of the southern African shrew Myosorex varius. J Biogeogr. 2011, 38: 1976-1991.
Barlow A, Baker K, Hendry CR, Peppin L, Phelps T, Tolley KA, Wüster CE, Wüster W: Phylogeography of the widespread African puff adder (Bitis arietans) reveals multiple Pleistocene refugia in southern Africa. Mol Ecol. 2013, 22: 1134-1157.
Trauth MH, Maslin MA, Deino A, Strecker MR: Late cenozoic moisture history of east Africa. Science. 2005, 309: 2051-2053.
Cohen AS, Stone JR, Beuning KRM, Park LE, Reinthal PN, Dettman D, Scholz CA, Johnson TC, King JW, Talbot MR, Brown ET, Ivory SJ: Ecological consequences of early late Pleistocene megadroughts in tropical Africa. P Natl Acad Sci USA. 2007, 104: 16422-16427.
Nittinger F, Gamauf A, Pinsker W, Wink M, Haring E: Phylogeography and population structure of the saker falcon (Falco cherrug) and the influence of hybridization: mitochondrial and microsatellite data. Mol Ecol. 2007, 16: 1497-1517.
Kotlík P, Deffontaine V, Mascheretti S, Zima J, Michaux JR, Searle JB: A northern glacial refugium for bank voles (Clethrionomys glareolus). P Natl Acad Sci USA. 2006, 103: 14860-14864.
Gómez A, Lunt DH: Refugia within refugia: patterns of phylogeographic concordance in the Iberian Peninsula. Phylogeography of southern European refugia. Edited by: Weiss S, Ferrand N. 2007, Netherlands: Springer, 155-188.
Murphy WJ, Collier GE: A molecular phylogeny for aplocheiloid fishes (Atherinomorpha, Cyprinodontiformes): the role of vicariance and the origins of annualism. Mol Biol Evol. 1997, 14: 790-799.
Wildekamp RH: A world of killies: atlas of the oviparous cyprinodontiform fishes of the world, vol. 4. 2004, Elyria, OH: American Killifish Association
Froese R, Pauly D: FishBase. [http://www.fishbase.org, version (08/2012)]
Watters BR: The ecology and distribution of Nothobranchius fishes. Journal of the American Killifish Association. 2009, 42: 37-76.
Terzibasi Tozzini E, Dorn A, Ng’oma E, Polačik M, Blažek R, Reichwald K, Petzold A, Watters BR, Reichard M, Cellerino A: Parallel evolution of senescence in annual fishes in response to extrinsic mortality. BMC Evol Biol. 2013, 13: article 77
Reichard M, Polačik M, Sedláček O: Distribution, colour polymorphism and habitat use of the African killifish, Nothobranchius furzeri, the vertebrate with the shortest lifespan. J Fish Biol. 2009, 74: 198-212.
Polačik M, Donner MT, Reichard M: Age structure of annual Nothobranchius fishes in Mozambique: is there a hatching synchrony?. J Fish Biol. 2011, 78: 796-809.
Valdesalici S, Cellerino A: Extremely short lifespan in the annual fish Nothobranchius furzeri. P Roy Soc B-Biol Sci. 2003, 270: 189-191.
Polačik M, Reichard M: Indirect fitness benefits are not related to male dominance in a killifish. Behav Ecol Sociobiol. 2009, 63: 1427-1435.
Reichard M: Nothobranchius kadleci (Cyprinodontiformes: Nothobranchiidae), a new species of annual killifish from central Mozambique. Zootaxa. 2010, 2332: 49-60.
Vanschoenwinkel B, Waterkeyn A, Nhiwatiwa T, Pinceel T, Spooren E, Geerts A, Clegg B, Brendonck L: Passive external transport of freshwater invertebrates by elephant and other mud-wallowing mammals in an African savannah habitat. Freshwater Biol. 2011, 56: 1606-1619.
Genade T, Benedetti M, Terzibasi E, Roncaglia P, Valenzano DR, Cattaneo A, Cellerino A: Annual fishes of the genus Nothobranchius as a model system for aging research. Aging Cell. 2005, 4: 223-233.
Jubb RA: A new Nothobranchius (Pisces, Cyprinodontidae) from Southeastern Rhodesia. Journal of the American Killifish Association. 1971, 8: 12-19.
Dorn A, Ng’oma E, Janko K, Reichwald K, Polačik M, Platzer M, Cellerino A, Reichard M: Phylogeny, genetic variability and colour polymorphism of an emerging animal model: the short-lived annual Nothobranchius fishes from southern Mozambique. Mol Phyl Evol. 2011, 61: 739-749.
Bandelt HJ, Forster P, Röhl A: Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol. 1999, 16: 37-48.
Chapuis MP, Estoup A: Microsatellite null alleles and estimation of population differentiation. Mol Biol Evol. 2007, 24: 621-631.
Raymond M, Rousset F: GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered. 1995, 86: 248-249.
Weir BS, Cockerham CC: Estimating F-statistics for the analysis of population structure. Evolution. 1984, 38: 1358-1370.
Belkhir K, Borsa P, Chikhi L, Raufaste N, Bonhomme F: GENETIX 4.05, Logiciel sous Windows TM pour la génétique des populations. 1996–2004, Montpellier (France): Laboratoire Génome, Populations, Interactions, CNRS UMR 5000, Université de Montpellier II
Hubisz MJ, Falush D, Stephens M, Pritchard JK: Inferring weak population structure with the assistance of sample group information. Mol Ecol Res. 2009, 9: 1322-1332.
Evanno G, Regnaut S, Goudet J: Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol. 2005, 14: 2611-2620.
Flanders J, Jones G, Benda P, Dietz C, Zhang SY, Li G, Sharifi M, Rossiter SJ: Phylogeography of the greater horseshoe bat, Rhinolophus ferrumequinum: contrasting results from mitochondrial and microsatellite data. Mol Ecol. 2009, 18: 306-318.
Jakobsson M, Rosenberg NA: CLUMPP: a cluster matching and permutation program for dealing with label switching and multimodality in analysis of population structure. Bioinformatics. 2007, 23: 1801-1806.
Rosenberg NA: Distruct: a program for the graphical display of population structure. Mol Ecol Notes. 2004, 4: 137-138.
Corander J, Marttinen P, Sirén J, Tang J: Enhanced Bayesian modeling in BAPS software for learning genetic structures of populations. BMC Bioinformatics. 2008, 9: article 539
Rousset F: Genetic differentiation and estimation of gene flow from F-statistics under isolation by distance. Genetics. 1997, 145: 1219-1228.
Beerli P, Felsenstein J: Maximum-likelihood estimation of migration rates and effective population numbers in two populations using a coalescent approach. Genetics. 1999, 1999 (152): 763-773.
Goudet J: FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). [http://www2.unil.ch/popgen/softwares/fstat.htm]
Cornuet JM, Luikart G: Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data. Genetics. 1996, 144: 2001-2014.
Piry S, Luikart G, Cornuet JM: BOTTLENECK: a computer program for detecting recent reductions in the effective population size using allele frequency data. J Hered. 1999, 90: 502-503.
Estoup A, Wilson IJ, Sullivan C, Cornuet JM, Moritz C: Inferring population history from microsatellite and enzyme data in serially introduced cane toads, Bufo marinus. Genetics. 2001, 159: 1671-1687.
Luikart G, Sherwin WB, Steele BM, Allendofr FW: Usefulness of molecular markers for detecting population bottlenecks via monitoring genetic change. Mol Ecol. 1998, 7: 963-974.
Librado P, Rozas J: DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics. 2009, 25: 1451-1452.
Ramos-Onsins SE, Rozas J: Statistical properties of new neutrality tests against population growth. Mol Biol Evol. 2002, 19: 2092-2100.
Excoffier L, Laval G, Schneider S: Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinform Online. 2005, 1: 47-50.
Rogers A: Genetic evidence for a Pleistocene population explosion. Evolution. 1995, 49: 608-615.
Schneider S, Excoffier L: Estimation of past demographic parameters from the distribution of pairwise differences when the mutation rates vary among sites: application to human mitochondrial DNA. Genetics. 1999, 152: 1079-1089.
Drummond AJ, Rambaut A, Shapiro B, Pybus OG: Bayesian coalescent inference of past population dynamics from molecular sequences. Mol Biol Evol. 2005, 22: 1185-1192.
Ho SYW, Shapiro B: Skyline-plot methods for estimating demographic history from nucleotide sequences. Mol Ecol Res. 2011, 11: 423-434.
Posada D: JModelTest: phylogenetic model averaging. Mol Biol Evol. 2008, 25: 1253-1256.
Drummond AJ, Ho SYW, Phillips MJ, Rambaut A: Relaxed phylogenetics and dating with confidence. PLoS Biol. 2006, 4: 699-710.
Ho SYW, Phillips MJ, Cooper A, Drummond AJ: Time dependency of molecular rate estimates and systematic overestimation of recent divergence times. Mol Biol Evol. 2005, 22: 1561-1568.
Kirschner J, Weber D, Neuschl C, Franke A, Bottger M, Zielke L, Powalsky E, Groth M, Shagin D, Petzold A, Hartmann N, Englert C, Brockmann GA, Platzer M, Cellerino A, Reichwald K: Mapping of quantitative trait loci controlling lifespan in the short-lived fish Nothobranchius furzeri - a new vertebrate model for age research. Aging Cell. 2012, 11: 252-261.
deMenocal PB: African climate change and faunal evolution during the Pliocene-Pleistocene. Earth Plan Sc Lett. 2004, 220: 3-24.
Vrba ES: Habitat theory in relation to the evolution in African neogene biota and hominids. African biogeography, climate change, & human evolution. Edited by: Bromage TG, Schrenk F. 1999, New York, USA: Oxford University Press, 34-
Linzey AV, Kesner MH, Chimimba CT, Newbery C: Distribution of veld rat sibling species Aethomys chrysophilus and A. ineptus (Rodentia: Muridae) in southern Africa. Afr Zool. 2003, 38: 169-174.
Markert JA, Schelly RC, Stiassny MLJ: Genetic isolation and morphological divergence mediated by high-energy rapids in two cichlid genera from the lower Congo rapids. BMC Evol Biol. 2010, 10: article 149
Remington CL: Suture-zones of hybrid interaction between recently joined biotas. Evolutionary biology. Edited by: Dobzhansky T, Hecht MK, Steere WC. 1968, New York: Plenum Press, 321-428.
Avise JC: Phylogeography. 2000, Cambridge, MA: Harvard University Press
Colangelo P, Granjon L, Taylor PJ, Corti M: Evolutionary systematics in African gerbilline rodents of the genus Gerbilliscus: inference from mitochondrial genes. Mol Phyl Evol. 2007, 42: 797-806.
Bryja J, Granjon L, Dobigny G, Patzenhauerová H, Konečný A, Duplantier JM, Gauthier P, Colyn M, Durnez L, Lalis A, Nicolas V: Plio-pleistocene history of west African sudanian savannah and the phylogeography of the Praomys daltoni complex (Rodentia): the environment/geography/genetic interplay. Mol Ecol. 2010, 19: 4783-4799.
Seehausen O: African cichlid fish: a model system in adaptive radiation research. P Roy Soc B-Biol Sci. 2006, 273: 1987-1998.
Johnson TC, Scholz CA, Talbot MR, Kelts K, Ricketts RD, Ngobi G, Beuning K, Ssemmanda I, McGill JW: Late Pleistocene desiccation of lake Victoria and rapid evolution of cichlid fishes. Science. 1996, 273: 1091-1093.
Terzibasi E, Valenzano DR, Benedetti M, Roncaglia P, Cattaneo A, Domenici L, Cellerino A: Large differences in aging phenotype between strains of the short-lived annual fish Nothobranchius furzeri. PLoS One. 2008, 3: article e3866
Jones FC, Grabherr MG, Chan YF, Russell P, Mauceli E, Johnson J, Swofford R, Pirun M, Zody MC, White S, Birney E, Searle S, Schmutz J, Grimwood J, Dickson MC, Myers RM, Miller CT, Summers BR, Knecht AK, Brady SD, Zhang H, Pollen AA, Howes T, Amemiya C, Lander ES, Di Palma F, Lindblad-Toh K, Kingsley DM, Broad Institute Genome Sequencing Platform & Whole Genome Assembly Team: The genomic basis of adaptive evolution in threespine sticklebacks. Nature. 2012, 484: 55-61.
Reichwald K, Lauber C, Nanda I, Kirschner J, Hartmann N, Schories S, Gausmann U, Taudien S, Schilhabel MB, Szafranski K, Glöckner G, Schmid M, Cellerino A, Schartl M, Englert C, Platzer M: High tandem repeat content in the genome of the short-lived annual fish Nothobranchius furzeri: a new vertebrate model for aging research. Genome Biol. 2009, 10: article R16
Valenzano DR, Kirschner J, Kamber RA, Zhang E, Weber D, Cellerino A, Englert C, Platzer M, Reichwald K, Brunet A: Mapping loci associated with tail color and sex determination in the short-lived fish Nothobranchius furzeri. Genetics. 2009, 183: 1385-1395.
Financial support came from Czech Science Foundation (laboratory work 2011–2012: P505/12/G112, fieldwork 2008–2011: 206/09/0815). We thank Milan Vrtílek and Peter Vallo for help in the field, Eileen Powalsky and Jeanette Kirschner for testing microsatellite primers in wild populations, Radka Poláková for designing PCR multiplex sets, and Carl Smith for comments on the manuscript.
The authors declare that they have no competing interests.
MR and JB conceived and designed the study, MR, MP, RB, VB and JB collected samples, VB conducted genotyping, VB, JB and KJ analysed the data, AC and KR contributed primers and samples of the captive GRZ strain, and JB, MR and VB wrote the paper. All authors read and approve the final manuscript.
Electronic supplementary material
Additional file 2: Pairwise F ST and isolation by distance. (a) Matrix of pairwise FST values calculated in Genetix. Highlighted values indicate pairs with non-significant FST values (i.e. p > 0.01). (b) Correlation between Ln(distance) and linearized pairwise FST values (FST /(1- FST)) tested with Mantel tests (1000 permutations). Isolation by distance was calculated separately for the three population groups and for the whole dataset. Red points indicate pairwise FST including most isolated localities in all three main groups; i.e. Pop323 in Chefu, Pop13 in LimpN, and Pop124 in LimpS. (XLSX 48 KB)
Additional file 3: Additions to analysis of population genetic structure. (a) Evaluation of 20 runs in STRUCTURE for each number of presumable clusters from K = 2 to K = 15. (i) Likelihood of models in STRUCTURE for increasing number of populations (K); (ii) Estimation of the best K division according to Evanno et al. (2005). The most supported division is for K = 2, but suitable models are represented also by K = 5, 7, 9, and 13. (b) Assignment of individuals to particular populations using models for K = 2 to 15 in STRUCTURE (based on 13 microsatellite loci). Codes for localities correspond to Figures 1 and 2, the names on the left indicate the mtDNA haplogroup. Population 406 with mtDNA from two haplogroups (Limpopo N and Chefu) is marked with a red arrow. (c) Spatial clustering of populations constructed in the program BAPS based on 13 microsatellite loci. Identical colours represent populations with similar genotypic composition. The best model suggests clustering into 13 different populations, therefore indicating strong genetic differentiation among the study populations. We also used suboptimal models to show the hierarchical structure of the sampled populations. (PPTX 5 MB)
About this article
Cite this article
Bartáková, V., Reichard, M., Janko, K. et al. Strong population genetic structuring in an annual fish, Nothobranchius furzeri, suggests multiple savannah refugia in southern Mozambique. BMC Evol Biol 13, 196 (2013) doi:10.1186/1471-2148-13-196
- Temporary pool
- Population genetics
- Pleistocene climate changes
- Founder effect