Figure 2

Phylogeny of DXR-II and DLO related sequences. ML cladogram depicting the evolutionary relationships among 53 DXR-II and 77 related protein sequences. Three clades defining main families are indicated. Statistical support on relevant clades is indicated by values next to nodes (ML aLRT support values/BA posterior probabilities/NJ bootstrap values). Sequence names are colored according to taxonomical groups (see legend). Sequence names include the bacterial strain name, followed by two pairs of square brackets: the first pair encloses the classification of the given bacterial strain according to the distribution of enzymes of the i) MEP and MVA pathways, left side of the vertical bar (i.e. classes A, +MEP pathway enzymes –DXR; B, +MEP pathway enzymes + DXR; C, -MEP + MVA pathway enzymes -DXR; D, +MEP + MVA pathway enzymes + DXR; E, -MEP -MVA pathway enzymes -DXR) and ii) CP pathway, right side of the vertical bar (i.e. A, complete CP pathway; B, incomplete CP pathway –AK_HD). The second pair of brackets represents the INTERPRO protein functional domains found i.e. 1, NAD(P)-binding domain (IPR016040); 2, Aspartate/homoserine dehydrogenase, NAD-binding (IPR005106); 3, Oxidoreductase, N-terminal (IPR000683); 4, Dihydrodipicolinate reductase, N-terminal (IPR000846); 5, Quinate/shikimate 5-dehydrogenase/glutamyl-tRNA reductase (IPR006151); 6, SAF domain (IPR013974). Asterisks indicate sequences for which DXR-II activity was previously tested through complementation assays [23] and Additional file 5.