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Table 1 Description of the 90 microarray CEL files used (accession number GEO NCBI database GSE33779 )

From: Suboptimal evolutionary novel environments promote singular altered gravity responses of transcriptome during Drosophilametamorphosis

Simulation facility

Temperature constraints (ΔT)

Oxygen (↓O2) constraints

g level

Name of CEL file replicates

RPM (From early to late pupae just before imagoes hatching)

No

No (open)

Sim μg^

80A

80B

80C

1g

81A

81B

81C

Yes

No (open)

Sim μg^

70A

70B

70C

1g

71A

71B

71C

Yes

Yes (close)

Sim μg^

70E

70F

70G

70H

1g

71E

71F

71G

71H

Magnetic levitator (From early to late pupae just before imagoes hatching)

No

Yes (close)

0g*

60A

60B

60C

1g*

61A

61B

61C

2g*

62A

62B

62C

1g

6cA

6cB

6cC

1g (open)

6oA

6oB

6oC

Yes

Yes (close)

0g*

50A

50B

50C

1g*

51A

51B

51C

2g*

52A

52B

52C

1g

5cA

5cB

5cC

1g (open)

5oA

5oB

5oC

Hypergravity centrifuge (6g/12g) (From early to late pupae just before imagoes hatching)

No

No (open)

12g^

Q4A

Q4B

---

6g^

---

Q3H#

Q3I#

1g^

Q0A

Q0H#

Q0I#

1g

Q1A

Q1H#

Q1I#

Yes

No (open)

12g^

R4A

R4B

R4C

6g^

---

R3H#

R3I#

1g^

R0A

R0H#

R0I#

1g

R1A

R1H#

R1I#

No

Yes (close)

12g^

Q9A#

Q9B#

Q9C#

6g^

---

Q8H#

Q8I#

1g^

QCA

QCB

QCC

1g

Q6A

Q6B

Q6C

Yes

Yes (close)

12g^

---

Q9E#

Q9F#

6g^

---

R8H#

R8I#

1g^

RCA

RCB

---

1g

R6A

R6B

---

  1. Comparison experiments were performed using different environmental constraints in three devices. ΔT constraints mean 3.5 days of cold step (12°C) then 4 days at 22°C following the GENE experiment temperature profile [1]. ↓O2 means oxygen amount limited by the container (closed). * indicates the presence of a high magnetic field; ^ indicates the presence of mechanical/rotational disturbances; # indicates that replicates have been performed in a parallel second experiment with the same design. The name assigned to any of CEL file replicates is provided for clarity when revisiting the dataset.