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Table 5 Competition among different site-directed mutants of bacteriophage Qβ in the presence of AZC

From: Evolution at increased error rate leads to the coexistence of multiple adaptive pathways in an RNA virus

Competitor viruses

Viruses present in the final population1

 

Transfer number 5

Transfer number 20

wt + QβA1746U

A1746U

ND2

wt + QβA2982G

A2982G

ND

wt + QβU3582C

U3582C

ND

A1746U + QβA2982G

A1746U

ND

A1746U + QβU3582C

A1746U

ND

A2982G + QβU3582C

A2982G +

A2982G +

U3582C

U3582C

A1746U + QβA1746U + A2982G

A1746U + A2982G

ND

A1746U + QβA1746U + U3582C

A1746U + U3582C

ND

A1746U + A2982G + QβA1746U + U3582C

A1746U + A2982G +

A1746U + A2982G + QβA1746U + U3582C

A1746U + U3582C

A1746U + QβA1746U + A2982G + QβA1746U + U3582C

(QβA1746U)3 +

(QβA1746U)3 +

A1746U + A2982G +

A1746U + A2982G +QβA1746U + U3582C

 

A1746U + U3582C

 
  1. 1Competitions were carried out as described in Methods. The viruses present in the populations obtained at the transfer indicated were identified through the determination of the consensus sequences at the nucleotide positions that distinguish the competitor viruses.
  2. 2ND means not determined.
  3. 3In this case, the determination of the consensus sequence do not allow distinguishing whether QβA1746U is present in the populations analyzed or not. We show this virus in brackets to indicate this uncertainty.