Fluorescence in situ hybridization of 5S rDNA sequences to metaphasic chromosomes of Asian and African cichlids. The 5S rDNA sequences were detected with FITC (green), the chromosomes were counterstained with DAPI, and the images were converted to grayscale. (a) Etroplus maculatus, (b) Oreochromis niloticus, (c) Tilapia mariae, (d) T. mamfe, (e) Hemichromis bimaculatus, (f) Gephyrochromis moorii, (g) Astatotilapia latifasciata, (h) Melanochromis auratus, (i) Pseudotropheus tropheus. The arrowheads indicate the 5S rDNA-bearing chromosomes. Bar = 5 μm.