- Research article
- Open Access
Polyphyletic origin of the genus Physarum(Physarales, Myxomycetes) revealed by nuclear rDNA mini-chromosome analysis and group I intron synapomorphy
BMC Evolutionary Biology volume 12, Article number: 166 (2012)
Physarales represents the largest taxonomic order among the plasmodial slime molds (myxomycetes). Physarales is of particular interest since the two best-studied myxomycete species, Physarum polycephalum and Didymium iridis, belong to this order and are currently subjected to whole genome and transcriptome analyses. Here we report molecular phylogeny based on ribosomal DNA (rDNA) sequences that includes 57 Physarales isolates.
The Physarales nuclear rDNA sequences were found to be loaded with 222 autocatalytic group I introns, which may complicate correct alignments and subsequent phylogenetic tree constructions. Phylogenetic analysis of rDNA sequences depleted of introns confirmed monophyly of the Physarales families Didymiaceae and Physaraceae. Whereas good correlation was noted between phylogeny and taxonomy among the Didymiaceae isolates, significant deviations were seen in Physaraceae. The largest genus, Physarum, was found to be polyphyletic consisting of at least three well supported clades. A synapomorphy, located at the highly conserved G-binding site of L2449 group I intron ribozymes further supported the Physarum clades.
Our results provide molecular relationship of Physarales genera, species, and isolates. This information is important in further interpretations of comparative genomics nd transcriptomics. In addition, the result supports a polyphyletic origin of the genus Physarum and calls for a reevaluation of current taxonomy.
Myxomycetes (plasmodial slime molds) are eukaryotic microorganisms that according to Olive  represent one of four main groups of slime molds (Mycetozoans). The myxomycetes consist of about 850 assigned species classified into five orders (Physarales, Stemonitales, Trichiales, Liceales and Echinosteliales) . A typical myxomycete species has a complex sexual life cycle that consists of two vegetative stages; a haploid unicellular stage (amoeba/ flagellate) and a diploid syncytium stage (plasmodium), as well as several dormant and developmental stages [3–5]. Myxomycetes are commonly found in nature on decaying plant materials where they feed on a variety of bacteria and unicellular eukaryotes as well as dissolved plant nutrients [5–8]. Identification of species is mainly based on morphological characters including fruiting body structures and sporocarp colors, and lime deposition [6, 9]. More recently, nuclear DNA sequence markers have contributed in resolving relationships among and within taxonomic groups. The ribosomal DNA (rDNA) spacers , obligatory group I introns , ribosomal RNA (rRNA) genes [11–16], and the elongation factor-1α (EF-1α) gene  and have all been applied.
The order Physarales is of special interest since it contains the two best-studied myxomycete species at the biochemical and molecular levels (Physarum polycephalum and Didymium iridis) [6, 18]. One unusual feature among the Physarales is the linear multicopy nature of the nuclear rDNA minichromosome, which ranges in size from about 20 kb to 80 kb among species [19–22]. Whereas the rDNA minichromosome in D. iridis is only 21 kb and contains a single pre-rRNA transcription unit, a 60-kb palindromic rDNA with two transcription units has been characterized in P. polycephalum. A hallmark of Physarales rDNA minichromosomes is the presence of multiple group I introns within the rRNA coding regions. All isolates investigated to date contain at least two group I introns in the large subunit (LSU) rRNA gene, and high intron loads are exemplified in isolates of Fuligo septica and Diderma niveum which contain 12 and 21 intron insertions, respectively [11, 22–25], our unpublished results. Group I introns code for ribozymes (catalytic RNAs) that perform self-splicing by a common molecular mechanism based on a series of transesterification reactions . A group I ribozyme is organized into a well-defined and highly conserved RNA core structure that consists of three helical stacks referred to as the catalytic domain (P3, P7-P9), the substrate domain (P1, P2, P10), and the scaffold domain (P4-P6) .
Here we report the presence of 222 rDNA group I introns and molecular phylogeny of 57 Physarales isolates (2 families, 10 genera, 31 defined species) based on combined data sets of nuclear small subunit (SSU) and LSU rRNA gene sequences. The analysis supports a polyphyletic origin of the Physarum genus. Phylogenetic analysis of Physarales is of particular importance since two representative species are currently under whole genome and transcriptome analyses. Whereas P. polycephalum is sequenced at The Genome Institute – Washington University, our laboratory at University of Tromsø investigates D. iridis by deep sequencing technologies. Resolving relationships of genera or species within and between the two Physarales families (Physaraceae and Didymiaceae) are crucial in interpretations of comparative genome and transcriptome data.
Results and discussion
High load of group I introns in myxomycete rDNA
A characteristic feature of myxomycete rRNA gene sequences is the high load of autocatalytic group I introns [11, 12, 24, 25, 27, 28]. These introns have to be correctly identified by structural characterizations and removed from coding sequences prior to phylogenetic analysis. Myxomycete group I introns have been found at 23 different, but highly conserved, insertion sites , and several of the introns are diverse in sequence and highly complex in organization due to internal protein coding genes, large arrays of direct repeat motifs, and even additional catalytic RNA domains [11, 29].
The rDNA sequences included in this study (near complete SSU rRNA gene and partial LSU rRNA gene; Figure 1) were examined for the presence of group I introns. We observed a massive intron contents (231 group I introns in 81 isolates; Additional file 1: Table S1, Additional file 2: Table S2, and Additional file 3: Table S3). Myxomycetes belonging to the order Physarales (Physaraceae and Didymiaceae families) harbored the majority of intron insertions (222 introns). Here, 55 and 167 introns were recognized in 21 and 36 isolates of Physaraceae and Didymiaceae, respectively (Additional file 3: Table S2 and Additional file 4: Table S3). Interestingly, all Physarales isolates investigated contained two obligatory group I introns at positions L1949 and L2449 in the LSU rRNA gene. Introns at these sites have been found to be strictly vertically inherited , have probably gained an essential biological role in benefit for the host cell , and possess highly complex structure and organization features at the RNA and DNA levels (see below).
Relationships among the mycetozoan rDNA
According to Olive  the mycetozoans have been divided into four main groups of slime molds; the myxomycetes, dictyostelids, protostelids and acrasids. As an initial approach to investigate the relationships among the mycetozoans groups we performed molecular phylogeny based on unambiguously aligned SSU rRNA genes sequences (1028 nt positions) from 26 defined species representing the four mycetozoan groups as well as various amoebozoans (Table 1). These sequences were either retrieved directly from the sequence database (http://www.ncbi.nlm.nih.gov) or generated in our laboratory by PCR amplification using specific primers followed by Sanger sequence determination (see Methods).
Some significant findings are noted from the un-rooted maximum likelihood (ML) tree (Additional file 1: Figure S1). The dictyostelids and myxomycetes were both found to represent monophyletic groups supported by high bootstrap values for ML, maximum parsimony (MP), neighbour joining (NJ), and Bayesian inference (BAY) analyses. Furthermore, each of the five main myxomycete orders (Physarales, Stemonitales, Trichiales, Liceales and Echinosteliales) represented distinct groups within the plasmodial slime molds. These findings corroborate earlier studies on the phylogeny of slime molds [13–16, 30, 31]. Finally, Soliformovum irregulare (protostelid) and Acrasis rosea (acrasid) appeared both distantly related from each other and from the dictyostelids and myxomycetes. From this analysis we conclude that the apparent phenotypic similarity between the mycetozoan groups  is not reflected in the genetic relationship.
Relationships within the Didymiaceae
Data presented in Additional file 1: Figure S1 and previous studies [13–16] supported a monophyletic origin of the order Physarales among the myxomycetes. To gain deeper insights in the relationships among families, genera, species and isolates within the order Physarales we performed molecular phylogeny based on two different data sets of nuclear rRNA gene sequences (Figure 1). The first data set represents an alignment of 2522 nt positions consisting of a near complete SSU rRNA gene in combination with a segment of the LSU rRNA gene (SSU/LSU data set; Additional file 5: Figure S2). The second data set consists of the LSU rRNA gene segment only (765 bp LSU data set; Additional file 6: Figure S3 and Additional file 7: Figure S4).
A representative ML tree based on the SSU/LSU data set is presented in Figure 2. The ML tree includes 17 isolates (4 genera, 8 defined species) of the Didymiaceae family, and supported by NJ/MP/ML > 64% and BAY posterior probability > 0.85 (Table 2). The monophyly of the four genera (Didymium, Lepidoderma, Mucilago and Diderma) was supported by NJ/MP/ML > 93% and BAY posterior probability of 1.0 (Figure 2). Furthermore, phylogenetic analysis was performed on the LSU data set (Table 2) generated from 36 Didymiaceae isolates (4 genera, 17 defined species). A representative ML tree is presented in Figure 3A. This analysis is in general agreement with that of the SSU/LSU data set, but with less statistical support at basal nodes of the Didymium and Diderma genera. However, the Lepidoderma genus was strongly supported in both data sets (Figure 2 and Figure 3A). These findings corroborate more recent studies of Didymiaceae relationships [11, 12], and we conclude that rDNA-based phylogeny is well consistent with the current Didymiaceae taxonomy [2, 32].
Relationships within Physaraceae
Figure 2 presents molecular phylogeny of 15 isolates (4 genera, 13 defined species) of the family Physaraceae based on the SSU/LSU data set. These isolates were separated from those of Didymiaceae with strong statistical supports represented by NJ/MP/ML bootstraps > 96% and BAY posterior probabilities of 1.0 (Figure 2). Furthermore, an extended analysis including 21 Physaraceae isolates (6 genera; 16 defined species; Table 3) based on the LSU data set is presented in Figure 3B. A different clustering pattern was seen among the Physaraceae genera compared to that of Didymiaceae. The ten investigated Physarum species were found to intersperse among isolates belonging to genera Badhamia, Craterium, Fuligo, Leocarpus and Physarella (Figures 2 and 3B). Three main clades (Clade I to III) with strong statistical support (SSU/LSU data set: NJ/MP/ML bootstraps > 84%, BAY posterior probabilities of 1; LSU data set: NJ/MP/ML bootstraps > 96%, BAY posterior probabilities > 0.94) were found to harbor both Physarum and Badhamia isolates (Figure 3B).
Recent taxonomy updates recognize at least 80 and 20 species of Physarum and Badhamia, respectively [2, 32]. Myxomycete taxonomy has traditionally been based on morphological characteristics of sporocarps and fruiting bodies. However, it has been debated for decades what characters that unambiguously distinguish Physarum and Badhamia genera species [33, 34], and consequently some Physarum species in the current taxonomy has previously been assigned to the Badhamia genus and vice versa . A similar relationship based on rRNA gene sequence phylogeny involving one Badhamia and two Physarum isolates was recently noted, but not further commented . Our findings of a polyphyletic origin of the Physarum genus, with at least three phylogenetic clades interspersed with Badhamia isolates, strongly suggest a reevaluation of the current taxonomy to also include molecular data.
Intron synapomorphy supports Physarumclades
A different approach to validate the Physarum clades is structural analysis of the obligatory group I introns present in the LSU rRNA gene of all investigated Physarales isolates (Additional file 3: Table S2 and Additional file 4: Table S3). Both L1949 and L2449 introns have strict vertical inheritance pattern within the Physarales with potential as genetic markers [11, 24]. We analyzed structural features of L2449 introns at the RNA level in more detail, and representative secondary structure diagrams of the catalytic core are presented in Figure 4A-C. Whereas the approximately 120-nt catalytic core was found conserved in sequence and structure, large extensions of various lengths were observed in all peripheral paired segment regions (P1, P2, P5, P6, P8 and P9). An extreme case was noted in the L2449 intron (2483 nt) in P. didermoides (Figure 4C). Here, direct repeat motifs were found both within the 503 nt P2 extension and the 1268 nt P9 extension (Figure 4D), and exemplifies the complex structural nature of myxomycete group I introns.
The most surprising structural feature, however, was found directly at the guanosine binding site (G-binding site) within the P7 segment. The G-binding site is at the catalytic center of a group I ribozyme, is highly conserved among all group I introns, and contains a universally conserved G:C pair adjacent to a bulged nucleotide (C or A). The G-binding site follows strong sequence co-variation rules, and if the bulged nucleotide is a C the first pair of the P7 segment is always an A:U or a U:A pair . Figure 4A presents structures of Clade I introns (P. pusillum and B. melanospora) which have a U:A pair followed by a bulged C. Clade II introns (P. polycephalum and B. utricularis) have a slightly different G-binding site of an A:U pair followed by a bulged C (Figure 4B). A third G-binding site variant was noted in the Clade III introns (P. compressum and P. didermoides; Figure 4C). Similar to Clade II introns, the Clade III introns have a A:U as the first P7 pair, but followed by a bulged U. The latter feature is highly unusual among group I introns and to our knowledge the Physarum Clade III L2449 introns are the only examples among the more than 22.000 group I introns available in the sequence data base [36, 37]. The fact that G-binding site variants appears to correlate with sequence-based phylogeny suggests that the intron structure character represents a synapomorphic character that further supports a complex origin of the Physarum genus.
Partial and complete rRNA gene sequences have been obtained from a large number of myxomycete isolates and have proven to represent reliable phylogenetic markers. However, myxomycete rRNA gene sequencing is challenged by an extreme high load of group I intron elements, and we report in our data sets 222 introns interrupting coding sequences in Physarales isolates. Several of the introns have complex organisations and were thus difficult to identify at sequence level. Introns are typically located in highly conserved parts of the rRNA genes that directly interfere with the design of universal primers and may result in sampling biases in analysis approaches. We observed congruence between phylogeny and current taxonomy of the Didymiaceae isolates, but a different pattern was observed among the family Physaraceae and includes a polyphyletic origin of the genus Physarum. Three well supported clades containing a mixture of Physarum and Badhamia isolates were noted, suggesting reevaluation of the current taxonomy into new genera. These observations were further supported by an unusual synapomorphic character at the G-binding site of the L2449 group I introns.
Myxomycetes isolates and culturing
All new isolates reported were collected as sporocarps. Their geographical origin, mode of classification, name of the collector, and if a cell line was obtained, are listed in Additional file 8: Table S4. Cultivation of amoebae was essentially performed as previously described [12, 38].
DNA extraction and sequencing
Total DNA was extracted from growing amoebae as previously described  or directly from sporocarps without culturing (summarized in Additional file 8: Table S4). Here, DNA extraction was performed using Berlin Technologies instrument (PreCelly’s 24 tube # VK05). Two to five sporocarp heads were homogenized in 250 μl lysis buffer (4 M Guanidine thiocyanate, 50 mM Tris–HCl (pH 7.5), 10 mM EDTA, 2% SDS, 1% β-mercaptoethanol) at 5000 rpm in 30 seconds for 1 to 4 cycles. The lysed spores were treated twice with phenol-chloroform, and DNA was extracted by ethanol precipitation and re-suspended in TE buffer. The SSU and LSU rRNA gene segments were PCR-amplified from total DNA extracts in several overlapping fragments using multiple sets of specific oligonucleotides (contact SDJ for details). DNA sequences were performed on both strands using automatic sequencing (Big Dye terminator chemistry, Applied Biosystems, Foster City, CA, USA).
Three data sets based on nuclear rRNA sequences were used in the molecular phylogeny. The SSU dataset (1028 nucleotide positions), the combined SSU/LSU data set (2522 nucleotide positions), and the LSU data set (765 nucleotide positions) were manually aligned in BioEdit v.22.214.171.124  based on the secondary structure models of P. polycephalum and D. iridis[22, 40]. Phylogenetic trees were built with the methods of NJ applying the Jukes-Cantor nucleotide substitution model based on the number of nucleotide substitution per site , and MP using heuristic search with close-neighbor-interchange (CNI) level 3 and generation of 10 random initial trees, in MEGA version 4.0.2 ., as well as ML using PhyML interface  and nucleotide substitution models selected by jModelTest 0.1 package . The following substitution models were used in ML: TPM2uf + I + G (SSU data set), GTR + I + G (SSU/LSU dataset), TNef + I (LSU dataset; Didymiaceae) and GTR + I + G (LSU dataset; Physaraceae). The reliabilities of tree branching points for NJ, MP and ML trees were evaluated by bootstrap analyses (2000 replications). BAY analyses were performed to reconstruct phylogenetic trees from all datasets using MrBayes, version 3.1.2 [45, 46] with two independent runs and the Metropolis-coupled Markov chain Monte Carlo (MCMCMC) method. Here the evolutionary model GTR + I + G was used for all datasets. A total of 1,000,000 generations were run with sampling every 100 generations. For each dataset the standard deviation of split frequencies was below 0.015 at the end of the run. Twenty-five % of initial trees were discarded and a consensus tree with posterior probabilities was generated from the remaining 15,000 trees.
Olive LS, Stoianovitch C: The Mycetozoans. 1975, New York: Academic Press
Poulain M, Meyer M, Bozonnet J: Les Myxomycetes. 2011
Dee J: Genes and development in Physarum. Trends Genet. 1987, 3: 208-213.
Einvik C, Elde M, Johansen S: Group I twintrons: genetic elements in myxomycete and schizopyrenid amoeboflagellate ribosomal DNAs. J Biotechnol. 1998, 64: 63-74. 10.1016/S0168-1656(98)00104-7.
Stephenson SL, Fiore-Donno AM, Schnittler M: Myxomycetes in soil. Soil Biol Biochem. 2011, 43: 2237-2242. 10.1016/j.soilbio.2011.07.007.
Stephenson SL, Stempen H: Myxomycetes: a handbook of slime molds. 1994, Portland, Oregon: Timber
Bonner JT: Brainless behavior: a myxomycete chooses a balanced diet. Proc Natl Acad Sci USA. 2010, 107: 5267-5268. 10.1073/pnas.1000861107.
Ko TW, Stephenson SL, Hyde KD, Carlos R, Lumyong S: Patterns of occurrence of myxomycetes on lianas. Fungal Ecol. 2010, 3: 302-310. 10.1016/j.funeco.2009.11.005.
Takahashi K, Hada Y: Geographical distribution of myxomycetes on coniferous deadwood in relation to air temperature in Japan. Mycoscience. 2010, 51: 281-290. 10.1007/s10267-010-0044-9.
Martin MP, Lado C, Johansen S: Primers are designed for amplification and direct sequencing of ITS region of rDNA from myxomycetes. Mycologia. 2003, 95: 474-479. 10.2307/3761889.
Wikmark OG, Haugen P, Haugli K, Johansen SD: Obligatory group I introns with unusual features at positions 1949 and 2449 in nuclear LSU rDNA of Didymiaceae myxomycetes. Mol Phylogenet Evol. 2007, 43: 596-604. 10.1016/j.ympev.2006.11.004.
Wikmark OG, Haugen P, Lundblad EW, Haugli K, Johansen SD: The molecular evolution and structural organization of group I introns at position 1389 in nuclear small subunit rDNA of myxomycetes. J Eukaryot Microbiol. 2007, 54: 49-56. 10.1111/j.1550-7408.2006.00145.x.
Fiore-Donno AM, Berney C, Pawlowski J, Baldauf SL: Higher-order phylogeny of plasmodial slime molds (Myxogastria) based on elongation factor 1-A and small subunit rRNA gene sequences. J Eukaryot Microbiol. 2005, 52: 201-210. 10.1111/j.1550-7408.2005.00032.x.
Fiore-Donno AM, Meyer M, Baldauf SL, Pawlowski J: Evolution of dark-spored Myxomycetes (slime-molds): molecules versus morphology. Mol Phylogenet Evol. 2008, 46: 878-889. 10.1016/j.ympev.2007.12.011.
Fiore-Donno AM, Nikolaev SI, Nelson M, Pawlowski J, Cavalier-Smith T, Baldauf SL: Deep phylogeny and evolution of slime moulds (mycetozoa). Protist. 2010, 161: 55-70. 10.1016/j.protis.2009.05.002.
Fiore-Donno AM, Kamono A, Chao EE, Fukui M, Cavalier-Smith T: Invalidation of Hyperamoeba by transferring its species to other genera of Myxogastria. J Eukaryot Microbiol. 2010, 57: 189-196. 10.1111/j.1550-7408.2009.00466.x.
Hoppe T, Kutschera U: In the shadow of Darwin: Anton de Bary's origin of myxomycetology and a molecular phylogeny of the plasmodial slime molds. Theory Biosci. 2010, 129: 15-23. 10.1007/s12064-009-0079-7.
Aldrich HC, Daniel JW: Cell biology of Physarum and Didymium. 1982, New York: Academic
Vogt VM, Braun R: Structure of Ribosomal DNA in Physarum polycephalum. J Mol Biol. 1976, 106: 567-587. 10.1016/0022-2836(76)90252-7.
Ferris PJ: Structure and inheritance of Physarumribosomal DNA.PhD thesis. 1984, Ithaca, NY: Cornell University
Silliker ME, Collins OR: Non-mendelian inheritance of mitochondrial DNA and ribosomal DNA in the myxomycete, Didymium iridis. Mol Gen Genet. 1988, 213: 370-378. 10.1007/BF00339605.
Johansen S, Johansen T, Haugli F: Extrachromosomal ribosomal DNA of Didymium iridis: sequence analysis of the large subunit ribosomal RNA gene and sub-telomeric region. Curr Genet. 1992, 22: 305-312. 10.1007/BF00317926.
Vader A, Naess J, Haugli K, Haugli F, Johansen S: Nucleolar introns from Physarum flavicomum contain insertion elements that may explain how mobile group I introns gained their open reading frames. Nucleic Acids Res. 1994, 22: 4553-4559. 10.1093/nar/22.22.4553.
Lundblad EW, Einvik C, Rønning S, Haugli K, Johansen S: Twelve Group I introns in the same pre-rRNA transcript of the myxomycete Fuligo septica: RNA processing and evolution. Mol Biol Evol. 2004, 21: 1283-1293. 10.1093/molbev/msh126.
Nielsen H, Johansen SD: Group I introns: Moving in new directions. RNA Biol. 2009, 6: 375-383. 10.4161/rna.6.4.9334.
Vicens Q, Cech TR: Atomic level architecture of group I introns revealed. Trends Biochem Sci. 2006, 31: 41-51. 10.1016/j.tibs.2005.11.008.
Johansen S, Johansen T, Haugli F: Structure and evolution of myxomycete nuclear group I introns: a model for horizontal transfer by intron homing. Curr Genet. 1992, 22: 297-304. 10.1007/BF00317925.
Haugen P, Reeb V, Lutzoni F, Bhattacharya D: The evolution of homing endonuclease genes and group I introns in nuclear rDNA. Mol Biol Evol. 2004, 21: 129-140.
Johansen SD, Haugen P, Nielsen H: Expression of protein-coding genes embedded in ribosomal DNA. Biol Chem. 2007, 388: 679-686.
Pawlowski J, Burki F: Untangling the phylogeny of amoeboid protists. J Eukaryot Microbiol. 2009, 56: 16-25. 10.1111/j.1550-7408.2008.00379.x.
Lahr DJ, Grant J, Nguyen T, Lin JH, Katz LA: Comprehensive phylogenetic reconstruction of amoebozoa based on concatenated analyses of SSU-rDNA and actin genes. PLoS One. 2011, 6: e22780-10.1371/journal.pone.0022780.
Neubert H, Nowotny W, Baumann K: Die Myxomyceten. 1995, Gomaringen: Karlheinz Baumann Verlag
Alexopoulos CJ: Morphology, taxonomy, and phylogeny. Cell biology of Physarum and Didymium. Edited by: Aldrich HC, Daniel JW. 1982, New York: Academic Press, 3-21.
Clark J, Haskins EF, Stephenson SL: Biosystematics of the myxomycete Badhamia gracilis. Mycologia. 2003, 95: 104-108. 10.2307/3761967.
Michel F, Westhof E: Modelling of the three-dimensional architecture of group I catalytic introns based on comparative sequence analysis. J Mol Biol. 1990, 216: 585-610. 10.1016/0022-2836(90)90386-Z.
Zhou Y, Lu C, Wu Q-J, Wang Y, Sun Z-T, Deng J-C, Zhang Y: GISSD: group I intron sequence and structure database. Nucleic Acids Res. 2008, 36: D31-D37. 10.1093/nar/gkn052.
Gardner PP, Daub J, Tate JG, Moore BL, Osuch IH, Griffiths-Jones S, Finn RD, Nawrocki EP, Kolbe DL, Eddy SR, Bateman A: Rfam: Wikipedia, clans and the “decimal” release. Nucleic Acids Res. 2011, 39: D131-D145. 10.1093/nar/gkr609.
Johansen S, Elde M, Vader A, Haugen P, Haugli K, Haugli F: In vivo mobility of a group I twintron in nuclear ribosomal DNA of the myxomycete Didymium iridis. Mol Microbiol. 1997, 24: 737-745. 10.1046/j.1365-2958.1997.3921743.x.
Hall TA: BioEdit: a user friendly sequence alignment editor and analysis program for windows 95/98/NT. Nucl Acids Symp Ser. 1999, 41: 95-98.
Johansen T, Johansen S, Haugli FB: Nucleotide sequence of Physarum polycephalum small subunit ribosomal RNA as inferred from the gene sequence: secondary structure and evolutionary implications. Curr Genet. 1988, 14: 265-273. 10.1007/BF00376747.
Nei M, Kumar S: Molecular evolution and phylogenetics. 2000, New York: Oxford University Press
Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.
Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704. 10.1080/10635150390235520.
Posada D: jModelTest: phylogenetic model averaging. Mol Biol Evol. 2008, 25: 1253-1256. 10.1093/molbev/msn083.
Huelsenbeck JP, Ronquist F: MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics. 2001, 17: 754-755. 10.1093/bioinformatics/17.8.754.
Ronquist F, Huelsenbeck JP: MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics. 2003, 19: 1572-1574. 10.1093/bioinformatics/btg180.
We thank Dr. J. Clark (University of Kentucky, USA), Iolanda and Giovanni Manavella (Italy), Dr. G. Shipley (University of Texas, USA), Marianne Meyer (France) and Dr. P. Haugen (University of Tromsø, Norway) for providing various myxomycete sporocarps and cell-lines. Dr. Finn Haugli is acknowledged for comments on the manuscript. This work was supported by grants to SDJ from the Norwegian Research Council and the University of Tromsø.
The authors declare that they have no competing interests.
SCRN participated in rDNA sequencing and performed the phylogenetic analysis. KH collected natural isolates, performed culturing and DNA extraction, and participated in rDNA sequencing. DHC participated in the initial design of this study and in the phylogenetic analysis. EFH participated in the initial design of this study and in discussions of results. SDJ directed the research, performed intron analysis, and wrote the manuscript in collaboration with all authors. All authors read and approved the final manuscript.
Electronic supplementary material
Additional file 1: Figure S1. ML phylogenetic tree of Mycetozoa isolates and associated amoebozoa based on the SSU data set. The analysis was based on the alignment of 1028 nucleotide positions from 26 isolates (Table 1). The evolution model is TPM2uf + I + G. Tree reconstructions using NJ, MP, ML, or BAY resulted in essential identical tree topologies. Bootstrap values (> 50%) of 2000 replications are shown at the internal nodes (NJ/MP/ML). Node of high statistical support (bootstrap values of 100%) is indicated by red circle. BAY analysis consists of 1,000,000 generations with trees sampled every 100 generations. Branches with BAY posterior probabilities of 1.0 are represented as thick black lines. (EPS 419 KB)
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Nandipati, S.C., Haugli, K., Coucheron, D.H. et al. Polyphyletic origin of the genus Physarum(Physarales, Myxomycetes) revealed by nuclear rDNA mini-chromosome analysis and group I intron synapomorphy. BMC Evol Biol 12, 166 (2012) doi:10.1186/1471-2148-12-166
- Neighbour Join
- Slime Mold
- Physarum Polycephalum
- L2449 Group
- Physarales Isolate