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Figure 2 | BMC Evolutionary Biology

Figure 2

From: Multiple long-term, experimentally-evolved populations of Escherichia coliacquire dependence upon citrate as an iron chelator for optimal growth on glucose

Figure 2

Dependence of growth on glucose is due to citrate itself rather than the coincidental addition of sodium. The ratio and error for growth parameters was calculated by fitting a log-linear model with the strain:media interaction terms and block as the dependent variables. Both the relative (A) growth rate and (B) yield on glucose for the three lineages that were strongly stimulated by the addition of sodium citrate (black circles) could be achieved by simply adding citric acid (white diamonds). Values represent the mean and 95% confidence intervals for the ratio of growth of that strain on glucose with the supplement to growth without.

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