Table 2 Oligonucleotide primers and sequences
Visual opsin genea, reference geneb, or plasmidc | Primer Name | Primer Sequence (5'→3') | Amplicon size (bp) and GenBank accession number | Primers designed against GenBank accession number or reference |
|---|---|---|---|---|
LWS A180 a | A180SpecExon2 | GGGTTTACAACGTCTCCACTC | (1017) | [8] |
| Â | A1803'UTR-DS | CAATGCAACTATGTTCATT | HQ260679 | DQ168659 |
LWS P180 a | pExon2 | GATGGGTTTACGATGTCGCAACGG | (1020) | [8] |
| Â | LWS2 IntRev | CAGTCCCGGCAGTAATAACAAAC | HQ260680 | Â |
LWS S180 a | a/sExon2 | GATGGGTTTACAACGTCTCCACAC | 1032 | [8] |
| Â | LWS1 IntRev | CATTTGCCATAAAGTTTCCGTTTATC | HQ260681 | Â |
LWS S180r a | S180rEx1-Ex2span-S | GCAATCATACAAGAGATCC | (1082) | [35] |
| Â | S180r3'UTR-S | ACACAAAACCTTCACTTTTAAGC | HQ260682 | Â |
RH1 a | RH1-F | ATGCATGGCTACTTTGTCC | (538) | DQ912024 |
| Â | RH1-S-R | GTGTGAAGATGTACCAGGCCACAC | HQ260686 | Â |
RH2-1 a | RH21-F | CATGGTGGACCCAATGATCTA | (827) | DQ234859 |
| Â | RH21-R | AGATAACAGGATTGTAAAGG | HQ391990 | Â |
RH2-2 a | RH22-F | CAGATCCATGGCAATTCAAA | (738) | DQ234858 |
| Â | RH22-R | TAAAGAAAATCCACGCAGCA | HQ260683 | Â |
SWS1 a | SWS1-F | TGCAGGCGCTCTTCATGG | (799) | DQ234861 |
| Â | SWS1-R | ATGAGCGGGTTGTAGAC | HQ260685 | Â |
SWS2B a | SWS2B-F | AGGGAGCCTGGGGCTTTTT | (803) | DQ234860 |
| Â | SWS2B-R | TGGAGGCTTTTGAGACACAG | HQ391991 | Â |
SWS2A a | SWS2A-F | GGCACTTCCATCAACACC | (480) | FJ711159 |
| Â | SWS2A-R | GAAGCAGAAAAGGAACATGACG | HQ260684 | Â |
COI b * | Cyt-c-F | GAGCCCTTAAATGGGAGACC | (445) | ES373762 |
| Â | Cyt-c-R | GCTGCGAAAGCTTCTCAAAT | HQ391989 | Â |
β-actin b | β-actin-F | GAAACCGGTTCCCTTAAAGC | (439) | EU143772.1 |
|  | β-actin-R | GGGGTGTTGAAGGTCTCAAA | HQ260687+ |  |
Myosin-HC b | Myosin-HC-F | ACACCAGCCTTCTGAACACC | (491) | ES371087 |
| Â | Myosin-HC-R | CTCGGCCTGTCTCTTGTAGG | HQ260688 | Â |
pGEM-T c | SP6 | TATTTAGGTGACACTATAG | MCS (141) + Insert | Promega® |
pGEM-T c | T7 | TAATACGACTCACTATAGGG | Â | Â |