Duplications in the NLRP2/7 locus and an NLRP2/7 paralog retrogene insertion event presage the emergence of POP2. A. Phylogenic analysis of POP2-like PYD protein sequences. Colored boxes represent groups of closely related protein sequences based on either grouping with the annotated human sequence (orthologs; black, NLRP7; blue, NLRP2; yellow CLRX/NOD24; or white, POP2) or groups between those identified by homology with their likely orthologs (purple, NLRP2/7; green, NLRP2-like; and beige, POP2-like). Numbers indicate bootstrap values (1000 replicates). B. Phylogenic analysis (maximum likelihood method) of MUSCLE aligned cDNA and predicted full-length NLRP2/7-related nucleotide sequences. The highest log likelihood tree (-14072.9433) is shown (1947 positions). Solid bars indicate grouping of likely orthologs. Numbers indicate bootstrap values. (Note: Macaque ChrX has good homology to CLRX.1 in its Pyrin domain (Figures 2A and 4A) but the full-length Macaque ChrX DNA sequence groups with Dog-XM_541420.2 (bootstrap value 35) and the alignment of the full-length DNA sequence is poor relative to the other genes). C. Graphic representation of NLRP2/7-related genes present in the species examined. Colors correspond with those in A. Marmoset NLRP2/NOD24 (beige) corresponds to contig 6464-5.