Study | Ts: Tv | Ts:Tv freq | 1 bp deletions | Del:BPS freq | 1 bp insertions | Other |
|---|
CDEII | 46: 68 | 0.68 | 4 | 0.047 | 0 | 18a |
Genome-wide (WT) | 12: 19 | 0.63 | 1 | 0.032 | 0 | 1 |
URA3 Lang (WT) | 46: 121 * | 0.38 | 22 | 0.017 | 3 | 15 |
CAN1 Lang (WT) | 65: 85 | 0.76 | 56 | 0.034 | 8 | 13 |
URA3 Hicks (WT) | 19: 27 | 0.70 | 5 | 0.014 | 1 | 9 |
URA3 Hicks (GC) | 32: 24 * | 1.33 | 32 | 0.074 | 1 | 14 |
- The mutation spectrum of CDEII is compared to the wild type spontaneous mutation (WT) inferred from various studies (Genome-wide, [15]; Lang, [23]; Hicks, [13]), and the mutation spectrum expected under gene conversion (GC, [13]). "Other" indicates indels that are -> 1 bp long, and in the case of CDEII also includes indels occurring in homopolymer runs that were 5 bp or longer (N = 14), other mutations described as "Other" for the WT and GC datasets are described in [13]. The deletion: base pair subsitution frequency (Del:BPS) corrects for the difference in target length at which deletions could be observed compared to the target length for base pair substitutions ([23], e.g. for URA3, indel target size = 804 bp, and BPS target size = 104 bp). For CDEII the number of deletions (N = 4) was compared to the number of base pair substitutions (N = 84) outside homopolymer runs. * Significantly different from CDEII (Fisher's exact test, P < 0.05). Poisson tests showed that none of the Del:BPS frequencies were significantly different from that of CDEII.
