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Table 1 List of primers used to isolate and characterize SCD1 and SCD5 genes in S. canicula and P. senegalus, and expression primers in S. canicula and G. aculeatus.

From: The evolutionary history of the stearoyl-CoA desaturase gene family in vertebrates

Designation

Sequence

Use

SCD5F1deg

5'CACCCCTTCACCTGGHTNTGGGCNTA3'

Isolation of PCR fragment

SCD5R2deg

5' CCAGGCCCAGCCAGMACATRAARTC 3'

Isolation of PCR fragment

SCD5R1deg

5' CGCTCCTTCCGGGCYTSDATCAT 3'

Isolation of PCR fragment

SCD5F2

5'TGTCACGTGGTTGGTGAACAGTGCT3'

Isolation of PCR fragment

SCD5_3Race

5'TCCAGCTGAACCCAACAACATGCTTTA3'

RACE PCR

SCD5_5Race

5' GTTTCCGAAGAAACAGCCAGCCAAT 3'

RACE PCR

SCD5Fexp

5'CCAATTCCATGGCTTTTCAG 3'

gene expression

SCD5Rexp

5'ACACAACAACAGGGTCAGCA3'

gene expression

SCD1F

5'TCGCCAACACCATGGCNTTYCA 3'

Isolation of PCR fragment

SCD1R

5'GAGTAGTCGTAGGGGAAGGTGTGRTGRTARTT 3'

Isolation of PCR fragment

SCD1_3Race

5'GTTGATGTGCTTCATCATCCCCACAGT 3'

RACE PCR

SCD1_5Race

5'ACGAGTTCCAGAGAGTTTCACCCCAGA 3'

RACE PCR

SCD1FSc

5'CTTGCTTAGTGCCCTTGGAG3'

gene expression

SCD1RSc

5' AACGTGCGAGAAGAAAAAGC3'

gene expression

GaSCD1aF

5'GGCCCTATGACAAGAGCATC3'

gene expression

GaSCD1aR

5'CGTAGTCAAAGGGGAACGTG3'

gene expression

GaSCD1bF

5'GATGCTCAACGCCACCTG3'

gene expression

GaSCD1bR

5'AAGGGGAATGTGTGGTGGTA3'

gene expression

SCD1Ffull

5' GGTGTATGCCCGTTCCTTT 3'

ORF PCR

SCD1Rfull

5' TGCGTCAGTGACTCGACAGT 3'

ORF PCR

SCD5Ffull

5' TGGTGAATATGGAGAATCAGGA 3'

ORF PCR

SCD5Rfull

5' TGGACTTTGAGATTTTCTTCGTG 3'

ORF PCR

ScActinf1

5' AGTTGGATGGGTCAGAAAGAC 3'

normalisation

ScActinR1

5' ACGCTCAGTCAGGATCTTCATC 3'

normalisation

GaActinF

5' TCCCTGGAGAAGAGCTACGA 3'

normalisation

GaActinR

5' GTGTTGGCGTACAGGTCCTT 3'

normalisation