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Table 3 List of primers used in this study.

From: New insights into the evolution of subtilisin-like serine protease genes in Pezizomycotina

Primer Primer Sequence
NP 5-AATG(A/C)T(G/T)(A/T)(C/T)GAACGGCCT(C/T)A-3
NR 5-TTAAGC(G/A)(G/T)(A/T/C)(G/T)CC(G/A)TTGTAG-3
P246 5-AA(G/A)TA(C/T)AT(C/T)GTCGTC(C/T)(A/T)(C/G)AAG-3
1480TSP1 5-ATGAGAGATGCGGTCAAGGC-3
1480TSP2 5-CGACTTCAGGCGAGTTCAGAATC-3
1480TSP3 5-CAAAGCCGCCAGTGTATCCAG-3
22TSP1 5-TGAGAGATGCGGTCAAGAC-3
22TSP2 5-TATCCTGAAGAGTAGCAGCGTCG-3
22TSP3 5-GGCGTGGAGAGATGAAATGCG-3
  1. NP and NR were used to amplify the full-length encoding genes of subtilisin-like serine proteases from A. musiformis and A. yunnanensis. P246 and NR were used to amplify the 3' fragment of the subtilisin-like serine protease genes from M. coelobrochum, M. psychrophilum and D. shizishanna. 1480TSPN (N = 1, 2, 3) and 22TSPN (N = 1, 2, 3) were used to amplify the 5' fragment sequences from M. coelobrochum and D. shizishanna by using the DNA Walking Speedup™ PreMix Kit (Seegene, Korea), respectively