Figure 6From: Retrotransposition and mutation events yield Rap1 GTPases with differential signalling capacityAnalysis of activation of mRap1A-retro1, -retro2 and hRap1B-retro. Cos7 cells were transfected with empty vector, 0.1, 0.5 or 2 μg Rap1A (Rap1A), 0.1, 0.5 or 2 μg Rap1 retrogenes or 0.1, 0.5 or 2 μg Rap1A-V12. After 48 hours cells were lysed, and Rap1-GTP was isolated using GST-RalGDS-RBD and detected by using Western blotting with an anti-HA antibody, indicated as PD: pull down. The expression of HA-Rap1 was determined by Westernblotting using anti-HA as a probe, indicated as TL: total lysate.Back to article page