Figure 5

Sequence conservation among the classes of tCWCH2 sequence motifs. Amino acid sequences were aligned and consensus sequences were generated (see Methods). Mizf ZF1-2, Mizf ZF3-4, and Zap1/ZafA ZF1-2 and ZF3-4 are indicated separately. The PDOC00028 C2H2 consensus is shown at the bottom as a general C2H2 consensus sequence (indicated by the tandem repeat of the consensus sequence). The short insertion sequences listed below were initially located at the sites indicated by the colored arrowheads in the alignments described above, but were separated to allow more comprehensive analyses. These sequences represent the longer linker sequences and the insertion of extra sequences described in the Results section. To achieve this analysis we omitted the four sequences AAWT01013938 (Schmidtea mediterranea Aebp2), CAG05504 (Tetraodon nigroviridis Gli), XP_001602003 (Nasonia vitripennis Gli), and XP_785526 (Strongylocentrotus purpuratus Gli) because these sequences contain exceptionally divergent sequences that disrupt the alignments.