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Table 3 Primers used for PCR amplification and sequencing

From: Haplowebs as a graphical tool for delimiting species: a revival of Doyle's "field for recombination" approach and its application to the coral genus Pocillopora in Clipperton

Marker

Primer name

Purpose

Sequence

Reference

ITS2 (nuclear)

ITSc2-5

PCR + sequencing

5'-AGCCAGCTGCGATAAGTAGTG-3'

[42]

ITS2 (nuclear)

R28S1

PCR + sequencing

5'-GCTGCAATCCCAAACAACCC-3'

[42]

ATPSβ (nuclear)

ATPSβf5

PCR

5'-CCAAGGGTGGNAARATHGGT-3'

this article

ATPSβ (nuclear)

ATPSβr2

PCR + sequencing

5'-GGTTCGTTCATCTGACCATACAC-3'

[26]

ATPSβ (nuclear)

ATPSβf2

sequencing

5'-TGAAAGACAAGAGCTCCAAGGTA-3'

[26]

ATPSβ (nuclear)

ATPSβf4

sequencing

5'-GAGCTGGTGTTGGAAAGACTGT-3'

this article

ORF (mitochondrial)

FATP6.1

PCR + sequencing

5'-TTTGGGSATTCGTTTAGCAG-3'

[26]

ORF (mitochondrial)

RORF

PCR + sequencing

5'-SCCAATATGTTAAACASCATGTCA-3'

[26]

ORF (mitochondrial)

FORF

sequencing

5'-GTGCGCCAGCATTCTATTG-3'

this article

ORF (mitochondrial)

RORF2

sequencing

5'-TAGAATGCTGGCGCACATAA-3'

this article

CR (mitochondrial)

FNAD5.2deg

PCR + sequencing

5'-GCCYAGRGGTGTTGTTCAAT-3'

[26]

CR (mitochondrial)

RCOI3deg

PCR + sequencing

5'-CGCAGAAAGCTCBARTCGTA-3'

[54]

CR (mitochondrial)

FNC1

sequencing

5'-GGGGTGAGATGAAGAGGTGA-3'

this article

CR (mitochondrial)

RNC1

sequencing

5'-CGGGTGCCACTATGTTTTCT-3'

this article