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Table 3 List of primers and reference genes (names, function and amplicon size) under investigation. The primers for the gene of interest (COI) are also included.

From: Insights into the role of differential gene expression on the ecological adaptation of the snail Littorina saxatilis

Gene

Forward primer

Reverse Primer

Function

Product size (bp)

18S

5'-GGTTTTCGGAACACGAGGTA-3'

5'-TGGCATCGTTTATGGTCAGA-3'

Small ribosomal subunit

200

α Tubulin

5'-CCATACCCTTCACCGACGTA-3'

5'-AGGTGGGCATCAACTACCAG-3'

Structural constituent of cytoskeleton

190

Histone H3.3

5'-AGAGTGCTCCCTCAACTGGA-3'

5'-GTCCTCAAAGAGACCCACCA-3'

To mark active chromatin (nucleosome structure)

194

Elongation Factor 1α

5'-GCCCTTGAACCACTTCATGT-3'

5'-ATCATCGGCGTCAACAAGAT-3'

Translational elongation

195

Elongation Factor 2

5'-ACGCATGTTCTCCTCACACA-3'

5'-CGCTACCTGGTGGACAACTT-3'

Translational elongation

198

Calmodulin

5'-CACCGTTCGTTTCATCCATA-3'

5'-GTTCTGTCCCAGCGACCTC-3'

Calcium-binding protein

182

COI

5'-GGGGGAGGAGACCCTATTCT-3'

5'-ATGGTGGGCCCATACAATAA-3'

Energy production (electron transport chain)

204