Ancient origin of animal U-box ubiquitin ligases
© Marín; licensee BioMed Central Ltd. 2010
Received: 24 June 2010
Accepted: 27 October 2010
Published: 27 October 2010
The patterns of emergence and diversification of the families of ubiquitin ligases provide insights about the evolution of the eukaryotic ubiquitination system. U-box ubiquitin ligases (UULs) are proteins characterized by containing a peculiar protein domain known as U box. In this study, the origin of the animal UUL genes is described.
Phylogenetic and structural data indicate that six of the seven main UUL-encoding genes found in humans (UBE4A, UBE4B, UIP5, PRP19, CHIP and CYC4) were already present in the ancestor of all current metazoans and the seventh (WDSUB1) is found in placozoans, cnidarians and bilaterians. The fact that only 4 - 5 genes orthologous to the human ones are present in the choanoflagellate Monosiga brevicollis suggests that several animal-specific cooptions of the U box to generate new genes occurred. Significantly, Monosiga contains five additional UUL genes that are not present in animals. One of them is also present in distantly-related protozoans. Along animal evolution, losses of UUL-encoding genes are rare, except in nematodes, which lack three of them. These general patterns are highly congruent with those found for other two families (RBR, HECT) of ubiquitin ligases.
Finding that the patterns of emergence, diversification and loss of three unrelated families of ubiquitin ligases (RBR, HECT and U-box) are parallel indicates that there are underlying, linage-specific evolutionary forces shaping the complexity of the animal ubiquitin system.
In eukaryotes, protein ubiquitination is a key biochemical mechanism involved in multiple cellular processes, which range from its main role in the control of protein quality and protein levels, as part of the ubiquitin-proteasome system, to the regulation of gene expression [1–3]. Understanding the groups of proteins that are either involved in ubiquitinating or in regulating the ubiquitination system is therefore highly significant. Among these proteins, ubiquitin ligases (also known as E3s) are particularly interesting. In all eukaryotes, E3s are numerous, structurally diverse and, most relevant from a functional point of view, they provide specificity to the ubiquitination process . In the last years, the problem of characterizing how the groups of ubiquitin ligases have emerged has attracted a significant degree of attention. In particular, my group has contributed to the determination of the evolutionary history and patterns of diversification of two classes of stand-alone ubiquitin ligases: 1) the RBR family, a particular kind of ring finger-containing proteins characterized by having a RING1 - IBR - RING2 domain signature [4–8]; and, 2) the HECT family, which includes all proteins with HECT domains . In humans, these families respectively have 15 and 28 members [5, 9]. We also analyzed the proteins of the cullin family, which are essential units of ubiquitin ligase complexes. This family includes 8 different human proteins .
In some cases, the origin of these E3s can be traced back to early eukaryotic evolution. For example, three of the twelve subfamilies of the RBR family (called Ariadne, ARA54 and Helicase) [4, 7, 8] or the three main types of cullin proteins  are present in both animals and plants. On the contrary, many other proteins have been found to be of more recent origin, e. g. they are animal-specific. When the analyses are focused on the metazoan lineage, a particular pattern has been found in both the RBR and the HECT families, consisting in two distinct processes: 1) an early diversification, in such a way that animals as different as a placozoan, an anemone and a human have basically the same repertoire, and often a similar number, of ubiquitin ligases of a given family; 2) losses of a substantial number of ubiquitin ligases in a few animal lineages, especially in nematodes and urochordates, and to a lesser extent in insects [7, 9].
To further determine the generality of this pattern, I decided to undertake the study of another important type of E3 proteins, the U-box ubiquitin ligases (UULs). UULs were discovered more than ten years ago . They are characterized by having a protein domain, the U box, which is structurally related to the RING finger, typical of many other ubiquitin ligases [12, 13]. Evidence for several of these proteins to be biochemically acting as ubiquitin ligases, both initiating and elongating ubiquitin chains, was soon obtained [11, 14, 15]. There are at least seven UUL-encoding genes in humans, namely the two very similar UBE4A and UBE4B genes (sometimes respectively called UFD2b and UFD2a), CHIP (also known as STUB1), UIP5 (a. k. a. UBOX5), PRP19 (a. k. a. PRPF19, SNEV), CYC4 (a. k. a. PPIL2, Cyp-60) and WDSUB1 [11, 12, 14]. Recently, an eighth gene, ACT1 (a. k. a. TRAF3IP2) has been described as encoding a ubiquitin ligase that may contain a very divergent U box .
Functional information for the roles of the products of these genes is variable, from almost non-existent to extensive. The best-known protein is Chip, which acts both as a cochaperone, together with chaperones such as Hsc70, Hsp70 and Hsp90 [17, 18] and as a ubiquitin ligase, alone or as part of complexes that include other E3s, such as the RBR ubiquitin ligase parkin [15, 19, 20]. Recessive mutations in the parkin gene are a well-known cause of familial Parkinson disease, and therefore it is of significant interest that Chip is acting also on the products of several other familial Parkinson disease genes, such as SNCA (which encodes α-synuclein; [21, 22]) and LRRK2 [23, 24]. The involvement of Chip in interacting and/or ubiquitinating other proteins involved in neurodegenerative diseases, such as Tau and APP (both implicated in Alzheimer disease; [25–28]), Malin (another ubiquitin ligase, involved in Lafora disease; ) and, finally, ataxin-1 and ataxin-3 (associated respectively to spinocerebellar ataxia types 1 and 3; [30, 31]) indicates general roles in the nervous system. Consistent with a highly pleiotropic phenotype, CHIP null mutant mice show shortened life span, accelerated aging and anomalous oxidative stress and protein quality levels .
Much less is known of the functions of the rest of UULs. PRP19 encodes a splicing factor required for the activation of the spliceosome in both Saccharomyces cerevisiae  and mammals . It is part of an evolutionary conserved protein complex known as Nineteen/NTC in yeasts and Prp19/CDC5L in mammalian species [34, 35] Although is has been determined to have ubiquitin ligase activity , the precise roles linked to that activity are unknown. Yeast, Drosophila and mouse Prp19 null mutants are lethal [36–38]. Two other UUL-encoding genes, UBE4A and UBE4B, are also related to a yeast gene, Ufd2. This non-essential gene was first characterized as encoding a protein able to elongate polyubiquitin chains (E4 protein) . Evidence for ubiquitin ligase (E3) activity for the mouse Ube4a (Ufd2b) and Ube4b (Ufd2a) proteins was soon obtained . As described above for Chip, the Ube4a and Ube4b mouse proteins have been shown to interact with chaperones, such as, respectively, DnaJc7 and VCP/p97 [25, 39]. The substrates of Ube4a or Ube4b in mammals remain largely unknown, although some preliminary information has been already obtained (see e. g. [40, 41]). In the nematode Caenorhabditis elegans, the products of the orthologs of UBE4B and CHIP, called respectively Ufd-2 and Chn-1, form a complex with the chaperone Cdc-48 (homologous to mammalian VCP/p97) and ubiquitinate another chaperone, called Unc-45, which is required for correct muscle formation [42, 43]. It has been suggested that the same biochemical process occur in humans, in which VCP/p97 gain-of-function mutations lead to a syndrome leading to myopathy, bone anomalies and, often, dementia [43, 44]. Presence of human Ube4a protein has been described in multiple tissues while mouse Ube4b protein was detected in cardiac muscle during development and mostly in the nervous system in adults [39, 45, 46]. Whether the cardiac muscle phenotype and the results described above for worm and mammalian muscles are related is unknown. Consistent with those data, mice harboring loss-of-function Ube4b mutations in homozygosis die before birth, while heterozygotes show multiple nervous system anomalies that worsen with age . Overexpression of mouse UBE4B also leads to neurodegeneration in parallel to the generation of ubiquitin inclusions . CYC4, originally called Cyp-60, was first characterized as encoding a divergent cyclophilin, which was only much later shown to be also a UUL [14, 48]. Some cyclophilins work as cochaperones, and in fact Cyc4 proteins has been shown to interact with the chaperone Hsp90 . The biochemical functions of Cyc4 are still poorly understood. None of the two potential biological effects described so far for Cyc4 have been related to the protein acting as a UUL [49, 50]. In C. elegans, the ortholog of CYC4, called mog-6, is involved in the switch from spermatogenesis to oogenesis in hermaphrodite worms . The last UUL for which there is some information is Uip5, which has been shown also to interact with the VCP/p97 protein, mentioned above . The roles of WDSUB1 are totally unknown.
Research in UUL proteins and UUL-encoding genes in animals would benefit by having them included in an evolutionary framework, which provides three different types of data. First, the patterns of presence/absence of the genes in different species, useful not only to understand the evolution of the genes, but also to determine the best animal models and eventually to better understand the phenotypes found in mutants (e. g. potential redundancies among closely related proteins). Second, the speed of the evolution of a same gene in different species, which may suggest either differences in their evolutionary constraints or new functional roles. Finally, the patterns of evolution of the structures of the proteins, which, if changes are present, may lead also to the generation of novel functional hypothesis, testable in the laboratory. Here, I describe the evolutionary history of all UUL-encoding genes in animals, using when needed external data, most especially from the genome of the closest animal relative sequenced so far, the choanoflagellate Monosiga brevicollis. I show that these genes follow the same evolutionary pattern as other E3s: diversification in early animal history followed by simplification in particular lineages. In most groups, these UUL-encoding genes are very conservative, but, as described for other ubiquitin ligase families (see above), nematode species turn out once again to be exceptional, given that they have lost three UUL-encoding genes. The functional implications of these results are discussed.
To generate comprehensive databases of UULs, TblastN searches were performed using the sequences of the human U-box proteins as queries against the nr, htgs, gss, est and wgs databases of the National Center for Biotechnology Information (NCBI) . The sequences obtained were aligned using Clustal X 2.07  to determine the regions of highest similarity within each gene (see below). All subsequent searches were focused on these conserved regions, the rest of the sequences were discarded. To obtain the final, exhaustive databases, TblastN searches against the same databases described above, using as queries the conserved regions of orthologous genes of several divergent species, were performed until no additional sequences were found. In that way, all the UUL sequences that either belonged to animal species or to the choanoflagellate Monosiga brevicollis present in the databases (circa April 2010) were selected. Monosiga can be used as a convenient outgroup to establish the genes that were already present when animals emerged, given that choanoflagellates are considered the closest living relatives of animals . From this dataset, only complete or almost complete sequences, which could be aligned along the whole length or at least a large fraction of the conserved regions, were kept for the phylogenetic analyses. However, additional, specific TblastN searches were performed to characterize whether fragments of UULs in basal animal species (such as placozoans or cnidarians) or from lophotrochozoan invertebrates (for which sequence data is limited) existed which could have been missed before. Also, nematode-specific searches were performed at the Nembase4 web page [55, 56] to confirm the conclusions of loss of UUL-encoding genes in these organisms. Once all the gene-specific databases were finished, each group of orthologous sequences was aligned using again Clustal X 2.07 and the alignments were manually corrected with GeneDoc 2.7 .
While this paper was being revised, the final draft genome sequence of the sponge Amphimedon queenslandica was reported . Given the interest in including sequences of this species, several of them unavailable in my original searches, I performed additional TblastN searches against the same NCBI databases indicated above. These searches were finished in September 2010. Seven genes or gene fragments were detected in A. queenslandica that were incorporated to generate final alignments.
From those alignments, phylograms were obtained using three different methods of phylogenetic reconstruction. First, Neighbor-joining (NJ) trees were characterized using MEGA 4 . Second, Maximum-parsimony (MP) trees were obtained using PAUP* 4.0, beta 10 version . Finally, maximum-likelihood (ML) trees were obtained using PHYML 3.0 . The parameters used were in general the same detailed in . However, two minor changes were made to refine the analyses: 1) for MP, the maximum number of tied trees was increased from 20 to 100 and the tree-bisection-reconnection algorithm, which is more exhaustive and precise than the subtree pruning-regrafting method used in , was chosen; 2) For ML, the improved Le and Gascuel matrix of amino acidic substitutions  was used instead of the older Blosum62 matrix. In all the analyses, 1000 bootstrap replicates were performed to establish the reliability of the NJ and MP trees. For ML, which is much more computer intensive, 100 bootstrap replicates were obtained. Finally, structural analyses of UUL proteins were performed with InterProScan .
Chip: Chip proteins are characterized by containing 3 N-terminal tetratricopeptide repeats (TPRs), involved in protein-protein interactions, and a C-terminal U-box (Figure 1). The gene Chip is ancient, being detectable in plants, fungi and multiple protozoan species ( and unpublished observations). Figure 2 shows the tree with the complete/almost complete Chip sequences detected in animal species. In all species for which genomic data is extensive, a single Chip gene is found. Figure 2 includes just two lophotrochozoan species, namely the platyhelminthes Schistosoma japonicum and S. mansoni. However, fragments of sequences with high similarity with Chip were detected in the genomes of many other lophotrochozoans, as molluscs (Aplysia, Euprymna and Crassostrea genera) and annelids (Capitella, Tubifex, Hirudo). Exceptionally, the gene was not found in the choanoflagellate M. brevicollis. In the alignments, it was particularly noticeable the high level of sequence divergence in the genes of Caenorhabditis nematode species. This is obvious in Figure 2: notice the "normal" position of the closely related nematode Brugia malayi, while Caenorhabditis sequences are separated from the rest of the tree and have much longer branches. Given that Drosophila and Caenorhabditis are both ecdysozoans, their divergence from the lineage that gave rise to humans occurred at the same time. Therefore, one would expect to find similar degrees of divergence when the human proteins are compared to either the fly or nematode proteins. However, for Chip that expectation is not fulfilled. Along the conserved region of 279 amino acids analyzed, the resemblance between human and Drosophila Chip proteins (identity 56%, similarity 76%) is much higher than the one found in the comparison human Chip vs. nematode Chip (identity: 38%, similarity: 58%).
Prp19: its origin also predates the emergence of animals, given that it is detectable in many other organisms, such as yeasts, where it was discovered  and plants [65, 66]. The PRP19 protein is characterized by having an N-terminal U box, followed by a specific region of homology  and 7 C-terminal WD40 repeats, also typical protein-protein interaction domains (Figure 1). As shown in Figure 3, single Prp19 genes are found in all animal species analyzed and in Monosiga brevicollis. Additional partial sequences in molluscs (Lottia, Aplysia) and annelids (Platynereis, Capitella), were also detected. As described above for Chip, nematode Prp19 protein sequences (this time including also the one in Brugia) appear as very divergent in the tree (Figure 3). Again, the resemblance between human and Drosophila melanogaster proteins along the conserved region examined for Prp19 (504 amino acids) was substantially higher (65% identity, 81% similarity) than the similitude between human and Caenorhabditis elegans (51% identity; 69% similarity).
CYC4: CYC4 proteins are characterized by having an N-terminal U box and a cyclophilin (a. k. a. Pro_isomerase) domain near to their C terminus (Figure 1). This type of proteins has been described in fungi  and plants , so their origin is also ancient. Figure 6 summarizes the phylogenetic analyses for the sequences of all CYC4 proteins detected in animals. Again, a single gene is found in all species, from sponges to humans. It is also present in M. brevicollis. Additional fragments of similar sequences were detected in molluscs (Aplysia, Lottia), annelids (Alvinella, Helobdella) and platyhelminthes (Schmidtea). Notably, once again nematode sequences appear in the tree as highly divergent (Figure 6). This time however, the human/D. melanogaster and human/C. elegans comparisons do not show a large discrepancy (Hs/Dm: identity 55%, similarity 76%; Hs/Ce: identity 51%, similarity 67%. Length of conserved sequence: 452 amino acids).
UIP5: The structure of the protein encoded by this gene (Figure 1) is peculiar in two ways. First, the U box is found in all the rest of UULs very close to their N- or C-terminal ends, but here is found in the middle of the protein. Also, UIP5 contains a second domain typical of ubiquitin ligases, the RING finger. This last feature could be interpreted as the U box being dispensable for UIP5 acting as a ubiquitin ligase. However, this is not true. The presence of the U box, but not of the RING finger, is required for the E3 activity of the protein . Also, the U box of UIP5 has been shown to mediate interactions with the ubiquitin-conjugating (E2) enzymes . The evolution of this gene has never been studied before. I have not detected UIP5 outside the animals, even Monosiga lacks one. It is very unlikely that this is due to distant orthologs being very divergent and thus difficult to detect, given that the C-terminal sequences of UIP5 used to screen the databases are very characteristic, long (336 amino acids, including the U box) and well conserved within animals. Therefore, Figure 7 is a good summary of all the organisms in which UIP5 exists. Most animals, including sponges, contain a UIP5 gene, but, interestingly, nematodes such as Caenorhabditis and Brugia have lost it. In lophotrochozoans, UIP5 genes have been detected in molluscs (Aplysia, Lottia), but not in annelids or platyhelminthes. A potential duplicate is observed in the tetraploid amphibian Xenopus laevis (Figure 7).
WDSUB1: the protein encoded by this gene is characterized by containing 7 WD40 repeats and a SAM_2 domain in addition of the U box (Figure 1). SAM domains, as WD40 repeats, are involved in protein-protein interactions. The gene has not been analyzed from an evolutionary point of view in previous studies. I found that bona fide WDSUB1 genes are restricted to animals. Figure 8 contains an alignment that has been obtained including in most cases the most C-terminal WD40 repeat (which is highly conserved), the SAM domain and the U box (as schematized in Figure 1). However, there are three exceptions. First, Monosiga brevicollis contains a gene clearly related to WDSUB1 in sequence (e. g. it has a very similar U box) but not in structure, given that it can be deduced that it encodes a protein with 2 SAM domains but without WD40 repeats (red dot in Figure 8). Second, I found a sequence of the sponge Amphimedon that encodes for a protein with 6 WD40 repeats and a U box, very similar to the one in WDSUB1 proteins, but lacks SAM domains (white dot). Finally, a few sequences having just the SAM and U box domains, but lacking the WD40 domain, have been also detected (black dots in Figure 8). Curiously, two of them come from the genomes of species of two other sponges (Oscarella, Leucetta). Thus, WDSUB1-related genes appeared before the emergence of animals, but bona fide WDSUB1 genes, encoding proteins with the characteristic WD40 - SAM - U box signature, seem to have originated more recently, after the ancestors of sponges diverged from the ancestor of the rest of animals. Notably, WDSUB1 genes are again absent in nematodes (Caenorhabditis, Brugia) and also in the fruit flies of the Drosophila genus, although they are present in some other insect species (Figure 8). They have neither been found in any lophotrochozoan species for which genomic data are available. Therefore, of all the UUL-encoding genes, WDSUB1 is clearly the one most prone to be lost. Finally, a duplication has been detected in some fish species (see also Figure 8).
Discussion and Conclusions
The evolutionary analysis of the available UUL sequences makes clear the patterns of emergence of the genes that encode these proteins. Four main results have been obtained. The first result is that animal UUL-encoding genes are ancient. Seven of the genes today found in our species appeared before the emergence of animals (Chip, PRP19, CYC4, which are present in many other eukaryotic groups) or in early animal evolution (UIP5, a bona fine WDSUB1 gene and the duplicates UBE4A and UBE4B. These last three genes actually derive from related genes already present before animals arose; Figure 10). The only exception is Act1, which encodes a protein that may contain a highly divergent U-box and is mammalian-specific. The second result is that the UUL-encoding genes are, as a whole, highly conserved in animals. The only exception is WDSUB1, which has been lost independently several times (Figure 10). The third main result is that nematodes have lost three UUL-encoding genes, something that has not occurred in any other lineage. In addition, the remnant nematode genes often have highly divergent sequences, which make them appear in abnormal positions in the phylogenetic trees (see Figures 2, 3, 5 and 6). This high level of divergence has been confirmed by comparing the level of similarity between orthologous Homo and Caenorhabditis proteins with the level found in the corresponding Homo/Drosophila comparisons. The expectation is that both levels are identical but, in all cases, the nematode proteins are more divergent, and often much more so (see Results). The final result concerns the unexpectedly large number of UUL-encoding genes found in choanoflagellates but not in animals, a total of five (Figure 9). One of them, Mb_UBOXa, turns to be an ancient gene that was lost in early animal evolution.
It is notable how these patterns are often coincidental with those found for RBR and HECT ubiquitin ligase-encoding genes [7, 9]. First, most genes of those two families are also either very ancient, being present in most/all eukaryotes, or, alternatively, arose in early animal evolution. In all cases, animals as simple as a placozoan or a cnidarian have sets of genes that are largely the same found in humans. In addition, the structural diversity of RBR and HECT proteins has remained basically the same since animals emerged. A second main result is that losses of RBR and HECT genes in particular lineages, and most especially in nematodes, are also observed. If we put together the results for the three E3 families (RBR, HECT and U-box), it is found that, out of 38 genes present before the cnidarian/bilaterian split, 20 have been lost in Caenorhabditis elegans, while only 2 are not present in the anemone Nematostella vectensis or in our own species. This result points to an extreme, general streamlining of the ubiquitin system in nematodes. Urochordates, such as Ciona intestinalis, are a second group in which both RBR and HECT E3s have been abundantly lost (8 out of 31 genes are missing). However, all UUL-encoding genes are present in Ciona (Figure 10), so this pattern is not as general as the one detected in nematodes. Finally, four HECT choanoflagellate-specific genes have been also described , suggesting that choanoflagellates have often independently increased their number of ubiquitin ligases. As indicated in the Introduction, several UULs have roles in the mammalian nervous system. However, given their presence in sponges or placozoans, which lack that system, it can be safely concluded that these roles must have been acquired relatively recently respect to the origin of the UUL-encoding genes. However, the fact that both the sponge Amphimedon and the placozoan Trichoplax have many genes involved in typical neuronal functions [58, 74] suggests that many conditions required for the generation of a complex nervous system were already present in the last common ancestor of all animals. In this sense, the early origin of UULs parallels the emergence of other types of proteins which are critical in the function of complex animals, yet appear much earlier in evolutionary history (reviewed by [75–77]).
In conclusion, the analysis of the U-box ubiquitin ligases in animals confirms and extends our previous findings about the general patterns of diversification that explain the current diversity of E3 proteins in animals. An additional result that may have experimental interest is the detection of a general simplification of the ubiquitin ligase set of proteins in a model species such as C. elegans. This may facilitate a fast determination in the nematode of the functions of these proteins and perhaps of the ubiquitination system as a whole.
- Kerscher O, Felberbaum R, Hochstrasser M: Modification of proteins by ubiquitin and ubiquitin-like proteins. Annu Rev Cell Dev Biol. 2006, 22: 159-180. 10.1146/annurev.cellbio.22.010605.093503.View ArticlePubMedGoogle Scholar
- Mukhopadhyay D, Riezman H: Proteasome-independent functions of ubiquitin in endocytosis and signaling. Science. 2007, 315: 201-215. 10.1126/science.1127085.View ArticlePubMedGoogle Scholar
- Schwartz AL, Ciechanover A: Targeting proteins for destruction by the ubiquitin system: implications for human pathobiology. Annu Rev Pharmacol Toxicol. 2009, 49: 73-96. 10.1146/annurev.pharmtox.051208.165340.View ArticlePubMedGoogle Scholar
- Marín I, Ferrús A: Comparative genomics of the RBR family, including the Parkinson's disease-related gene parkin and the genes of the ariadne subfamily. Mol Biol Evol. 2002, 19: 2039-2050.View ArticlePubMedGoogle Scholar
- Marín I, Lucas JI, Gradilla AC, Ferrús A: Parkin and relatives: the RBR family of ubiquitin ligases. Physiol Genomics. 2004, 17: 253-263. 10.1152/physiolgenomics.00226.2003.View ArticlePubMedGoogle Scholar
- Lucas JI, Arnau V, Marín I: Comparative genomics and protein domain graph analyses link ubiquitination and RNA metabolism. J Mol Biol. 2006, 357: 9-17. 10.1016/j.jmb.2005.12.068.View ArticlePubMedGoogle Scholar
- Marín I: RBR ubiquitin ligases: Diversification and streamlining in animal lineages. J Mol Evol. 2009, 69: 54-64. 10.1007/s00239-009-9252-3.View ArticlePubMedGoogle Scholar
- Marín I: Diversification and specialization of plant RBR ubiquitin ligases. PLoS ONE. 2010Google Scholar
- Marín I: Animal HECT ubiquitin ligases: evolution and functional implications. BMC Evol Biol. 2010, 10: 56-10.1186/1471-2148-10-56.PubMed CentralView ArticlePubMedGoogle Scholar
- Marín I: Diversification of the cullin family. BMC Evol Biol. 2009, 9: 267-10.1186/1471-2148-9-267.PubMed CentralView ArticlePubMedGoogle Scholar
- Koegl M, Hoppe T, Schlenker S, Ulrich HD, Mayer TU, Jentsch S: A novel ubiquitination factor, E4, is involved in multiubiquitin chain assembly. Cell. 1999, 96: 635-644. 10.1016/S0092-8674(00)80574-7.View ArticlePubMedGoogle Scholar
- Aravind L, Koonin EV: The U box is a modified RING finger - a common domain in ubiquitination. Curr Biol. 2000, 10: R132-134. 10.1016/S0960-9822(00)00398-5.View ArticlePubMedGoogle Scholar
- Ohi MD, Vander Kooi CW, Rosenberg JA, Chazin WJ, Gould KL: Structural insights into the U-box, a domain associated with multi-ubiquitination. Nat Struct Biol. 2003, 10: 250-255. 10.1038/nsb906.View ArticlePubMedGoogle Scholar
- Hatakeyama S, Yada M, Matsumoto M, Ishida N, Nakayama KI: U box proteins as a new family of ubiquitin-protein ligases. J Biol Chem. 2001, 276: 33111-33120. 10.1074/jbc.M102755200.View ArticlePubMedGoogle Scholar
- Jiang J, Ballinger CA, Wu Y, Dai Q, Cyr DM, Höhfeld J, Patterson C: CHIP is a U-box-dependent E3 ubiquitin ligase: identification of Hsc70 as a target for ubiquitylation. J Biol Chem. 2001, 276: 42938-42944. 10.1074/jbc.M101968200.View ArticlePubMedGoogle Scholar
- Liu C, Qian W, Qian Y, Giltiay NV, Lu Y, Swaidani S, Misra S, Deng L, Chen ZJ, Li X: Act1, a U-box E3 ubiquitin ligase for IL-17 signaling. Sci Signal. 2009, 2: ra63-10.1126/scisignal.2000382.PubMed CentralPubMedGoogle Scholar
- Ballinger CA, Connell P, Wu Y, Hu Z, Thompson LJ, Yin LY, Patterson C: Identification of CHIP, a novel tetratricopeptide repeat-containing protein that interacts with heat shock proteins and negatively regulates chaperone functions. Mol Cell Biol. 1999, 19: 4535-4545.PubMed CentralView ArticlePubMedGoogle Scholar
- Connell P, Ballinger CA, Jiang J, Wu Y, Thompson LJ, Höhfeld J, Patterson C: The co-chaperone CHIP regulates protein triage decisions mediated by heat-shock proteins. Nat Cell Biol. 2001, 3: 93-96. 10.1038/35050618.View ArticlePubMedGoogle Scholar
- Murata S, Minami Y, Minami M, Chiba T, Tanaka K: CHIP is a chaperone-dependent E3 ligase that ubiquitylates unfolded protein. EMBO Rep. 2001, 2: 1133-1138. 10.1093/embo-reports/kve246.PubMed CentralView ArticlePubMedGoogle Scholar
- Imai Y, Soda M, Hatakeyama S, Akagi T, Hashikawa T, Nakayama KI, Takahashi R: CHIP is associated with Parkin, a gene responsible for familial Parkinson's disease, and enhances its ubiquitin ligase activity. Mol Cell. 2002, 10: 55-67. 10.1016/S1097-2765(02)00583-X.View ArticlePubMedGoogle Scholar
- Shin Y, Klucken J, Patterson C, Hyman BT, McLean PJ: The co-chaperone carboxyl terminus of Hsp70-interacting protein (CHIP) mediates alpha-synuclein degradation decisions between proteasomal and lysosomal pathways. J Biol Chem. 2005, 280: 23727-23734. 10.1074/jbc.M503326200.View ArticlePubMedGoogle Scholar
- Tetzlaff JE, Putcha P, Outeiro TF, Ivanov A, Berezovska O, Hyman BT, McLean PJ: CHIP targets toxic alpha-Synuclein oligomers for degradation. J Biol Chem. 2008, 283: 17962-17968. 10.1074/jbc.M802283200.PubMed CentralView ArticlePubMedGoogle Scholar
- Ding X, Goldberg MS: Regulation of LRRK2 stability by the E3 ubiquitin ligase CHIP. PLoS ONE. 2009, 4: e5949-10.1371/journal.pone.0005949.PubMed CentralView ArticlePubMedGoogle Scholar
- Ko HS, Bailey R, Smith WW, Liu Z, Shin JH, Lee YI, Zhang YJ, Jiang H, Ross CA, Moore DJ, Patterson C, Petrucelli L, Dawson TM, Dawson VL: CHIP regulates leucine-rich repeat kinase-2 ubiquitination, degradation, and toxicity. Proc Natl Acad Sci USA. 2009, 106: 2897-2902. 10.1073/pnas.0810123106.PubMed CentralView ArticlePubMedGoogle Scholar
- Hatakeyama S, Matsumoto M, Yada M, Nakayama KI: Interaction of U-box-type ubiquitin-protein ligases (E3s) with molecular chaperones. Genes Cells. 2004, 9: 533-548. 10.1111/j.1356-9597.2004.00742.x.View ArticlePubMedGoogle Scholar
- Petrucelli L, Dickson D, Kehoe K, Taylor J, Snyder H, Grover A, De Lucia M, McGowan E, Lewis J, Prihar G, Kim J, Dillmann WH, Browne SE, Hall A, Voellmy R, Tsuboi Y, Dawson TM, Wolozin B, Hardy J, Hutton M: CHIP and Hsp70 regulate tau ubiquitination, degradation and aggregation. Hum Mol Genet. 2004, 13: 703-714. 10.1093/hmg/ddh083.View ArticlePubMedGoogle Scholar
- Shimura H, Schwartz D, Gygi SP, Kosik KS: CHIP-Hsc70 complex ubiquitinates phosphorylated tau and enhances cell survival. J Biol Chem. 2004, 279: 4869-4876. 10.1074/jbc.M305838200.View ArticlePubMedGoogle Scholar
- Kumar P, Ambasta RK, Veereshwarayya V, Rosen KM, Kosik KS, Band H, Mestril R, Patterson C, Querfurth HW: CHIP and HSPs interact with beta-APP in a proteasome-dependent manner and influence Abeta metabolism. Hum Mol Genet. 2007, 16: 848-864. 10.1093/hmg/ddm030.View ArticlePubMedGoogle Scholar
- Rao SN, Sharma J, Maity R, Jana NR: Co-chaperone CHIP stabilizes aggregate-prone malin, a ubiquitin ligase mutated in Lafora disease. J Biol Chem. 2010, 285: 1404-1413. 10.1074/jbc.M109.006312.PubMed CentralView ArticlePubMedGoogle Scholar
- Choi JY, Ryu JH, Kim HS, Park SG, Bae KH, Kang S, Myung PK, Cho S, Park BC, Lee do H: Co-chaperone CHIP promotes aggregation of ataxin-1. Mol Cell Neurosci. 2007, 34: 69-79. 10.1016/j.mcn.2006.10.002.View ArticlePubMedGoogle Scholar
- Williams AJ, Knutson TM, Colomer Gould VF, Paulson HL: In vivo suppression of polyglutamine neurotoxicity by C-terminus of Hsp70-interacting protein (CHIP) supports an aggregation model of pathogenesis. Neurobiol Dis. 2009, 33: 342-353. 10.1016/j.nbd.2008.10.016.PubMed CentralView ArticlePubMedGoogle Scholar
- Min JN, Whaley RA, Sharpless NE, Lockyer P, Portbury AL, Patterson C: CHIP deficiency decreases longevity, with accelerated aging phenotypes accompanied by altered protein quality control. Mol Cell Biol. 2008, 28: 4018-4025. 10.1128/MCB.00296-08.PubMed CentralView ArticlePubMedGoogle Scholar
- Vijayraghavan U, Company M, Abelson J: Isolation and characterization of pre-mRNA splicing mutants of Saccharomyces cerevisiae. Genes Dev. 1989, 3: 1206-1216. 10.1101/gad.3.8.1206.View ArticlePubMedGoogle Scholar
- Ajuh P, Kuster B, Panov K, Zomerdijk JC, Mann M, Lamond AI: Functional analysis of the human CDC5L complex and identification of its components by mass spectrometry. EMBO J. 2000, 19: 6569-6581. 10.1093/emboj/19.23.6569.PubMed CentralView ArticlePubMedGoogle Scholar
- Tarn WY, Hsu CH, Huang KT, Chen HR, Kao HY, Lee KR, Cheng SC: Functional association of essential splicing factor(s) with PRP19 in a protein complex. EMBO J. 1994, 13: 2421-2431.PubMed CentralPubMedGoogle Scholar
- Cheng SC, Tarn WY, Tsao TY, Abelson J: PRP19: a novel spliceosomal component. Mol Cell Biol. 1993, 13: 1876-1882.PubMed CentralView ArticlePubMedGoogle Scholar
- Fortschegger K, Wagner B, Voglauer R, Katinger H, Sibilia M, Grillari J: Early embryonic lethality of mice lacking the essential protein SNEV. Mol Cell Biol. 2007, 27: 3123-3130. 10.1128/MCB.01188-06.PubMed CentralView ArticlePubMedGoogle Scholar
- Spradling AC, Stern D, Beaton A, Rhem EJ, Laverty T, Mozden N, Misra S, Rubin GM: The Berkeley Drosophila Genome Project gene disruption project: Single P-element insertions mutating 25% of vital Drosophila genes. Genetics. 1999, 153: 135-177.PubMed CentralPubMedGoogle Scholar
- Kaneko C, Hatakeyama S, Matsumoto M, Yada M, Nakayama K, Nakayama KI: Characterization of the mouse gene for the U-box-type ubiquitin ligase UFD2a. Biochem Biophys Res Commun. 2003, 300: 297-304. 10.1016/S0006-291X(02)02834-6.View ArticlePubMedGoogle Scholar
- Okumura F, Hatakeyama S, Matsumoto M, Kamura T, Nakayama KI: Functional regulation of FEZ1 by the U-box-type ubiquitin ligase E4B contributes to neuritogenesis. J Biol Chem. 2004, 279: 53533-53543. 10.1074/jbc.M402916200.View ArticlePubMedGoogle Scholar
- Hosoda M, Ozaki T, Miyazaki K, Hayashi S, Furuya K, Watanabe K, Nakagawa T, Hanamoto T, Todo S, Nakagawara A: UFD2a mediates the proteasomal turnover of p73 without promoting p73 ubiquitination. Oncogene. 2005, 24: 7156-7169. 10.1038/sj.onc.1208872.View ArticlePubMedGoogle Scholar
- Hoppe T, Cassata G, Barral JM, Springer W, Hutagalung AH, Epstein HF, Baumeister R: Regulation of the myosin-directed chaperone UNC-45 by a novel E3/E4-multiubiquitylation complex in C. elegans. Cell. 2004, 118: 337-349. 10.1016/j.cell.2004.07.014.View ArticlePubMedGoogle Scholar
- Janiesch PC, Kim J, Mouysset J, Barikbin R, Lochmüller H, Cassata G, Krause S, Hoppe T: The ubiquitin-selective chaperone CDC-48/p97 links myosin assembly to human myopathy. Nat Cell Biol. 2007, 9: 379-390. 10.1038/ncb1554.View ArticlePubMedGoogle Scholar
- Watts GD, Wymer J, Kovach MJ, Mehta SG, Mumm S, Darvish D, Pestronk A, Whyte MP, Kimonis VE: Inclusion body myopathy associated with Paget disease of bone and frontotemporal dementia is caused by mutant valosin-containing protein. Nat Genet. 2004, 36: 377-381. 10.1038/ng1332.View ArticlePubMedGoogle Scholar
- Contino G, Amati F, Pucci S, Pontieri E, Pichiorri F, Novelli A, Botta A, Mango R, Nardone AM, Sangiuolo FC, Citro G, Spagnoli LG, Novelli G: Expression analysis of the gene encoding for the U-box-type ubiquitin ligase UBE4A in human tissues. Gene. 2004, 328: 69-74. 10.1016/j.gene.2003.11.017.View ArticlePubMedGoogle Scholar
- Kaneko-Oshikawa C, Nakagawa T, Yamada M, Yoshikawa H, Matsumoto M, Yada M, Hatakeyama S, Nakayama K, Nakayama KI: Mammalian E4 is required for cardiac development and maintenance of the nervous system. Mol Cell Biol. 2005, 25: 10953-10964. 10.1128/MCB.25.24.10953-10964.2005.PubMed CentralView ArticlePubMedGoogle Scholar
- Susaki E, Kaneko-Oshikawa C, Miyata K, Tabata M, Yamada T, Oike Y, Katagiri H, Nakayama KI: Increased E4 activity in mice leads to ubiquitin-containing aggregates and degeneration of hypothalamic neurons resulting in obesity. J Biol Chem. 2010, 285: 15538-15547. 10.1074/jbc.M110.105841.PubMed CentralView ArticlePubMedGoogle Scholar
- Wang BB, Hayenga KJ, Payan DG, Fisher JM: Identification of a nuclear-specific cyclophilin which interacts with the proteinase inhibitor eglin c. Biochem J. 1996, 314: 313-319.PubMed CentralView ArticlePubMedGoogle Scholar
- Pushkarsky T, Yurchenko V, Vanpouille C, Brichacek B, Vaisman I, Hatakeyama S, Nakayama KI, Sherry B, Bukrinsky MI: Cell surface expression of CD147/EMMPRIN is regulated by cyclophilin 60. J Biol Chem. 2005, 280: 27866-27871. 10.1074/jbc.M503770200.View ArticlePubMedGoogle Scholar
- Espeseth AS, Huang Q, Gates A, Xu M, Yu Y, Simon AJ, Shi XP, Zhang X, Hodor P, Stone DJ, Burchard J, Cavet G, Bartz S, Linsley P, Ray WJ, Hazuda D: A genome wide analysis of ubiquitin ligases in APP processing identifies a novel regulator of BACE1 mRNA levels. Mol Cell Neurosci. 2006, 33: 227-235. 10.1016/j.mcn.2006.07.003.View ArticlePubMedGoogle Scholar
- Belfiore M, Pugnale P, Saudan Z, Puoti A: Roles of the C. elegans cyclophilin-like protein MOG-6 in MEP-1 binding and germline fates. Development. 2004, 131: 2935-2945. 10.1242/dev.01154.View ArticlePubMedGoogle Scholar
- NCBI. [http://0-www.ncbi.nlm.nih.gov.brum.beds.ac.uk/]
- Larkin MA, Blackshields G, Brown NP, Chenna R, McGettigan PA, McWilliam H, Valentin F, Wallace IM, Wilm A, Lopez R, Thompson JD, Gibson TJ, Higgins DG: Clustal W and Clustal X version 2.0. Bioinformatics. 2007, 23: 2947-2948. 10.1093/bioinformatics/btm404.View ArticlePubMedGoogle Scholar
- King N, Westbrook MJ, Young SL, Kuo A, Abedin M, Chapman J, Fairclough S, Hellsten U, Isogai Y, Letunic I, Marr M, Pincus D, Putnam N, Rokas A, Wright KJ, Zuzow R, Dirks W, Good M, Goodstein D, Lemons D, Li W, Lyons JB, Morris A, Nichols S, Richter DJ, Salamov A, Sequencing JG, Bork P, Lim WA, Manning G, Miller WT, McGinnis W, Shapiro H, Tjian R, Grigoriev IV, Rokhsar D: The genome of the choanoflagellate Monosiga brevicollis and the origin of metazoans. Nature. 2008, 451: 783-788. 10.1038/nature06617.PubMed CentralView ArticlePubMedGoogle Scholar
- Nembase 4. [http://www.nematodes.org/nembase4/]
- Wasmuth J, Schmid R, Hedley A, Blaxter M: On the extent and origins of genic novelty in the phylum Nematoda. PLoS Negl Trop Dis. 2008, 2: e258-10.1371/journal.pntd.0000258.PubMed CentralView ArticlePubMedGoogle Scholar
- Nicholas KB, Nicholas HB: GeneDoc: a tool for editing and annotating multiple sequence alignments. 1997, Distributed by the authorGoogle Scholar
- Srivastava M, Simakov O, Chapman J, Fahey B, Gauthier ME, Mitros T, Richards GS, Conaco C, Dacre M, Hellsten U, Larroux C, Putnam NH, Stanke M, Adamska M, Darling A, Degnan SM, Oakley TH, Plachetzki DC, Zhai Y, Adamski M, Calcino A, Cummins SF, Goodstein DM, Harris C, Jackson DJ, Leys SP, Shu S, Woodcroft BJ: The Amphimedon queenslandica genome and the evolution of animal complexity. Nature. 2010, 466: 720-726. 10.1038/nature09201.PubMed CentralView ArticlePubMedGoogle Scholar
- Tamura K, Dudley J, Nei M, Kumar S: MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol. 2007, 24: 1596-1599. 10.1093/molbev/msm092.View ArticlePubMedGoogle Scholar
- Swofford DL: PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4. 2003, Sinauer Associates, Sunderland, MassachusettsGoogle Scholar
- Guindon S, Gascuel O: A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. Syst Biol. 2003, 52: 696-704. 10.1080/10635150390235520.View ArticlePubMedGoogle Scholar
- Le SQ, Gascuel O: An improved general amino acid replacement matrix. Mol Biol Evol. 2008, 25: 1307-20. 10.1093/molbev/msn067.View ArticlePubMedGoogle Scholar
- Zdobnov EM, Apweiler R: InterProScan - an integration platform for the signature-recognition methods in InterPro. Bioinformatics. 2001, 17: 847-848. 10.1093/bioinformatics/17.9.847.View ArticlePubMedGoogle Scholar
- Yan J, Wang J, Li Q, Hwang JR, Patterson C, Zhang H: AtCHIP, a U-box-containing E3 ubiquitin ligase, plays a critical role in temperature stress tolerance in Arabidopsis. Plant Physiol. 2003, 132: 861-869. 10.1104/pp.103.020800.PubMed CentralView ArticlePubMedGoogle Scholar
- Wiborg J, O'Shea C, Skriver K: Biochemical function of typical and variant Arabidopsis thaliana U-box E3 ubiquitin-protein ligases. Biochem J. 2008, 413: 447-457. 10.1042/BJ20071568.View ArticlePubMedGoogle Scholar
- Monaghan J, Xu F, Gao M, Zhao Q, Palma K, Long C, Chen S, Zhang Y, Li X: Two Prp19-like U-box proteins in the MOS4-associated complex play redundant roles in plant innate immunity. PLoS Pathog. 2009, 5: e1000526-10.1371/journal.ppat.1000526.PubMed CentralView ArticlePubMedGoogle Scholar
- Grillari J, Ajuh P, Stadler G, Löscher M, Voglauer R, Ernst W, Chusainow J, Eisenhaber F, Pokar M, Fortschegger K, Grey M, Lamond AI, Katinger H: SNEV is an evolutionarily conserved splicing factor whose oligomerization is necessary for spliceosome assembly. Nucleic Acids Res. 2005, 33: 6868-6883. 10.1093/nar/gki986.PubMed CentralView ArticlePubMedGoogle Scholar
- Finn RD, Mistry J, Tate J, Coggill P, Heger A, Pollington JE, Gavin OL, Gunasekaran P, Ceric G, Forslund K, Holm L, Sonnhammer EL, Eddy SR, Bateman A: The Pfam protein families database. Nucleic Acids Res. 2010, 38: D211-D222. 10.1093/nar/gkp985.PubMed CentralView ArticlePubMedGoogle Scholar
- Azevedo C, Santos-Rosa MJ, Shirasu K: The U-box protein family in plants. Trends Plant Sci. 2001, 6: 354-358. 10.1016/S1360-1385(01)01960-4.View ArticlePubMedGoogle Scholar
- Pemberton TJ, Kay JE: Identification and Comparative Analysis of the Peptidyl-Prolyl cis/trans Isomerase Repertoires of H. sapiens, D. melanogaster, C. elegans, S. cerevisiae and Sz. pombe. Comp Funct Genomics. 2005, 6: 277-300. 10.1002/cfg.482.PubMed CentralView ArticlePubMedGoogle Scholar
- Yee D, Goring DR: The diversity of plant U-box E3 ubiquitin ligases: from upstream activators to downstream target substrates. J Exp Bot. 2009, 60: 1109-1121. 10.1093/jxb/ern369.View ArticlePubMedGoogle Scholar
- Pringa E, Martinez-Noel G, Muller U, Harbers K: Interaction of the ring finger-related U-box motif of a nuclear dot protein with ubiquitin-conjugating enzymes. J Biol Chem. 2001, 276: 19617-19623. 10.1074/jbc.M100192200.View ArticlePubMedGoogle Scholar
- Whittaker CA, Hynes RO: Distribution and evolution of von Willebrand/integrin A - domains: widely dispersed domains with roles in cell adhesion and elsewhere. Mol Biol Cell. 2002, 13: 3369-3387. 10.1091/mbc.E02-05-0259.PubMed CentralView ArticlePubMedGoogle Scholar
- Srivastava M, Begovic E, Chapman J, Putnam NH, Hellsten U, Kawashima T, Kuo A, Mitros T, Salamov A, Carpenter ML, Signorovitch AY, Moreno MA, Kamm K, Grimwood J, Schmutz J, Shapiro H, Grigoriev IV, Buss LW, Schierwater B, Dellaporta SL, Rokhsar DS: The Trichoplax genome and the nature of placozoans. Nature. 2008, 454: 955-960. 10.1038/nature07191.View ArticlePubMedGoogle Scholar
- Degnan BM, Vervoort M, Larroux C, Richards GS: Early evolution of metazoan transcription factors. Curr Opin Genet Dev. 2009, 19: 591-599. 10.1016/j.gde.2009.09.008.View ArticlePubMedGoogle Scholar
- Erwin DH: Early origin of the bilaterian developmental toolkit. Philos Trans R Soc Lond B Biol Sci. 2009, 364: 2253-2261. 10.1098/rstb.2009.0038.PubMed CentralView ArticlePubMedGoogle Scholar
- Marshall CR, Valentine JW: The importance of preadapted genomes in the origin of the animal bodyplans and the Cambrian explosion. Evolution. 2010, 64: 1189-1201.PubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.