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Table 2 Characteristics of microsatellite loci used to assess individual heterozygosity in southern dunlins (n = 76 individuals)

From: Trapped in the extinction vortex? Strong genetic effects in a declining vertebrate population

Locus*

No. of alleles

Allele size range (bp)

HO

HE

Calp2

8

127-147

0.684

0.711

Calp4

5

118-128

0.173†

0.451

Calp5

4

112-118

0.500

0.590

Ruff1

9

175-215

0.868

0.842

Ruff6

7

123-147

0.763

0.779

Ruff9

6

180-200

0.763

0.791

Ruff10

6

252-280

0.421†

0.649

PGT83‡

8

155-171

0.697

0.754

4A11

2

143-145

0.167

0.359

  1. For each locus, the name, the source species from which the locus was originally isolated and its reference are presented. Amplification results are shown as number of alleles, range of allele sizes in base pairs (bp), observed (HO) and expected heterozygosity (HE).
  2. *Locus name (source species, reference): Calp2-5 (Calidris alpina, [54]); Ruff1-10 (Philomachus pugnax, [55]); PGT83 (Calidris canutus; D.M. Buehler, A.J. Baker, unpublished data; GenBank Accession number AY198173); 4A11 (Haematopus ostralegus, [56]).
  3. †Significant deviation from Hardy-Weinberg Equilibrium (χ2 = 26.7, df = 1, p < 0.001, and χ2 = 9.5, df = 1, p < 0.01, respectively).
  4. ‡Primer sequence (5'-3'); F: AATCCGTTTCTGGGGACTGGG, R: TGCCTAATGCTGACTCACACC (A. Pauliny, unpublished data).
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BMC Ecology and Evolution

ISSN: 2730-7182

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