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Table 4 Primers used in cv-lysozyme 3 cDNA cloning, and semi-quantitative RT-PCR

From: A new lysozyme from the eastern oyster, Crassostrea virginica, and a possible evolutionary pathway for i-type lysozymes in bivalves from host defense to digestion

Primer Usage Direction Sequence
Lyso3-3' 3'-RACE - 5'- CTT ACG GAA GCG ATG CAC CCT GCA CGA AC-3'
Lyso3-5' 5'-RACE - 5'- CCG GTA GAA AAT GAG CCG GTG TGG GAG GC-3'
Lyso3-F RT-PCR Forward 5'- GAG TTT AAA CTG AAC TTA AG-3'
Lyso3-R RT-PCR Reverse 5'- CGT GAA CTT ATG TTT TAT TG-3'
28S-F RT-PCR Forward 5'- GTT GAC GCA ATG TGA TTT CTG C-3'
28S-R RT-PCR Reverse 5'-TAG ATG ACG AGG CAT TTG GCT A-3'