Table 4 Primers used in cv-lysozyme 3 cDNA cloning, and semi-quantitative RT-PCR

Lyso3-3'

3'-RACE

-

5'- CTT ACG GAA GCG ATG CAC CCT GCA CGA AC-3'

Lyso3-5'

5'-RACE

-

5'- CCG GTA GAA AAT GAG CCG GTG TGG GAG GC-3'

Lyso3-F

RT-PCR

Forward

5'- GAG TTT AAA CTG AAC TTA AG-3'

Lyso3-R

RT-PCR

Reverse

5'- CGT GAA CTT ATG TTT TAT TG-3'

28S-F

RT-PCR

Forward

5'- GTT GAC GCA ATG TGA TTT CTG C-3'

28S-R

RT-PCR

Reverse

5'-TAG ATG ACG AGG CAT TTG GCT A-3'