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Table 1 Properties of eastern oyster lysozymes.

From: A new lysozyme from the eastern oyster, Crassostrea virginica, and a possible evolutionary pathway for i-type lysozymes in bivalves from host defense to digestion

Characteristic

cv-lysozyme-3

cv-lysozyme-1*

cv-lysozyme-2**

Lysozyme activity

   

   Optimal pH

7.5-8.5

5.5-6.5

5.5-6.5

   Optimal ionic strength (I)

0.005-0.01

0.18-0.20

0.005-0.01

Antibacterial activity (MIC)

   

   E. coli

12.5 μg/mL

1.56 μg/mL

50 μg/mL

   V. vulnificus

ND***

12.5 μg/mL

100 μg/mL

Molecular properties

   

   MW (Dalton)b

17782.3

17861.0

12984.6

   Theoretical pI

6.84 (7.12)

8.95

6.33

Amino acid sequence

   

   Total amino acid number

168

164

117

   Arginine residue number

5

19

5

Protease cutting sites

   

   Trypsin

9

18

9

   Thermolysin

32

28

20

   Pepsin (pH > 2.0)

19

12

5

Major sites of gene expression

Digestive glands

Labial palps, mantle

Digestive glands

Tissues with low mRNA level

Mantle, labial palps, gill, style sac-midgut, hemocytes

Gill, style sac-midgut, digestive gland, gonad

Style sac-midgut

  1. *[56, 58]; ** [57]; *** Not detected at 100 μg/mL
  2. The biochemical and molecular characteristics of three mature proteins with lysozyme activities identified from the eastern oyster, Crassostrea virginica, were compared.