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Table 1 Properties of eastern oyster lysozymes.

From: A new lysozyme from the eastern oyster, Crassostrea virginica, and a possible evolutionary pathway for i-type lysozymes in bivalves from host defense to digestion

Characteristic cv-lysozyme-3 cv-lysozyme-1* cv-lysozyme-2**
Lysozyme activity    
   Optimal pH 7.5-8.5 5.5-6.5 5.5-6.5
   Optimal ionic strength (I) 0.005-0.01 0.18-0.20 0.005-0.01
Antibacterial activity (MIC)    
   E. coli 12.5 μg/mL 1.56 μg/mL 50 μg/mL
   V. vulnificus ND*** 12.5 μg/mL 100 μg/mL
Molecular properties    
   MW (Dalton)b 17782.3 17861.0 12984.6
   Theoretical pI 6.84 (7.12) 8.95 6.33
Amino acid sequence    
   Total amino acid number 168 164 117
   Arginine residue number 5 19 5
Protease cutting sites    
   Trypsin 9 18 9
   Thermolysin 32 28 20
   Pepsin (pH > 2.0) 19 12 5
Major sites of gene expression Digestive glands Labial palps, mantle Digestive glands
Tissues with low mRNA level Mantle, labial palps, gill, style sac-midgut, hemocytes Gill, style sac-midgut, digestive gland, gonad Style sac-midgut
  1. *[56, 58]; ** [57]; *** Not detected at 100 μg/mL
  2. The biochemical and molecular characteristics of three mature proteins with lysozyme activities identified from the eastern oyster, Crassostrea virginica, were compared.