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Table 2 Acetylation activity measured in tissue homogenates from heads of B. lanceolatum

From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

   blAANAT activity
(nmol/h/μg of total protein)
Time of day pH Endogenous substrate Exogenous + endogenous
substrates
Exogenous + endogenous
substrates
+ CoA-T
Midday 8.5 0.26 ± 0.15 0.68 ± 0.27 0.004 ± 0.002
Midnight   0.08 ± 0.08 0.05 ± 0.05 0.001 ± 0.001
Midday 9.5 0.29 ± 0.08 0.47 ± 0.17 0.004 ± 0.001
Midnight   Not detectable Not detectable Not detectable
  1. Comparison of samples collected during day and night. The exogenous substrate is 10 mM PEA; 1 mM CoA-T has been used for inhibition. Data are given as the mean ± S.E.M. (N = 3 to 6).