Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

Table 2 Acetylation activity measured in tissue homogenates from heads of B. lanceolatum

		blAANAT activity (nmol/h/μg of total protein)
Time of day	pH	Endogenous substrate	Exogenous + endogenous substrates	Exogenous + endogenous substrates + CoA-T
Midday	8.5	0.26 ± 0.15	0.68 ± 0.27	0.004 ± 0.002
Midnight		0.08 ± 0.08	0.05 ± 0.05	0.001 ± 0.001
Midday	9.5	0.29 ± 0.08	0.47 ± 0.17	0.004 ± 0.001
Midnight		Not detectable	Not detectable	Not detectable

Comparison of samples collected during day and night. The exogenous substrate is 10 mM PEA; 1 mM CoA-T has been used for inhibition. Data are given as the mean ± S.E.M. (N = 3 to 6).

ISSN: 2730-7182