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Table 1 Kinetic characteristics of GST-bfAANAT and GST-bfAANAT ' for selected amines.

From: Evolution of AANAT: expansion of the gene family in the cephalochordate amphioxus

  Substrate
  Phenylethylamine Tyramine Serotonin Tryptamine
bfAANAT α δ' α δ' α δ' α δ'
V max 17.8 ± 1.1 4.9 ± 3 2.06 ± 0.28 0.78 ± .06 1.60 ± 0.1 0.56 ± 0.06 4.7 ± 0.8 2.7 ± 0.25
K m 3 ± 0.6 45 ± 36 8.2 ± 2.70 0.80 ± 0.28 0.01 ± 0.01 <0.01 8.1 ± 3.6 2.5 ± 0.7
  Substrate
  Octopamine Propylamine Butylamine Putrescine
bfAANAT α δ' α δ' α δ' α δ'
V max 2.1 ± 0.2 0.80 ± 0.05 >10 0.73 ± 0.07 32.5 ± 4.1 0.7 ± 0.05 1.34 ± 0.32 5.11 ± 0.11
K m 6.8 ± 1.9 0.05 ± 0.01 >20 0.90 ± 0.37 47.5 ± 8.0 0.37 ± 0.23 5.2 ± 4.0 3.68 ± 0.22
  1. Activity was measured at pH 9.0. Values are calculated from data in Figure 7. V max values are nmol/h/pmol enzyme and are given as the mean ± S.E.M. (N = 3 to 6). For further details see Methods.